scholarly journals Fuwen Wei—Recipient of the 2021 Molecular Ecology Prize

2021 ◽  
Vol 31 (1) ◽  
pp. 31-36
Author(s):  
Yibo Hu ◽  
Louis Bernatchez
Keyword(s):  
Molecular Ecology

The ontogeny of molecular ecology

2006 ◽  
Vol 15 (10) ◽  
pp. 2687-2689 ◽  
Author(s):  
JOHN C. AVISE
Keyword(s):  
Molecular Ecology

scholarly journals Astrobiological Field Campaign to a Volcanosedimentary Mars Analogue Methane Producing Subsurface Protected Ecosystem: Imuruk Lake (Alaska)

2011 ◽  
Vol 2011 ◽  
pp. 1-8
Author(s):  
F. Gómez ◽  
O. Prieto-Ballesteros ◽  
D. Fernández-Remolar ◽  
J. A. Rodríguez-Manfredi ◽  
M. Fernández-Sampedro ◽  
...  
Keyword(s):  
Molecular Ecology ◽  
Point Of View ◽  
Land Bridge ◽  
High Hydrogen ◽  
Adverse Conditions ◽  
Life On Mars ◽  
Life Detection ◽  
National Preserve ◽  
Mars Exploration Rovers

Viking missions reported adverse conditions for life in Mars surface. High hydrogen signal obtained by Mars orbiters has increased the interest in subsurface prospection as putative protected Mars environment with life potential. Permafrost has attracted considerable interest from an astrobiological point of view due to the recently reported results from the Mars exploration rovers. Considerable studies have been developed on extreme ecosystems and permafrost in particular, to evaluate the possibility of life on Mars and to test specific automated life detection instruments for space missions. The biodiversity of permafrost located on the Bering Land Bridge National Preserve has been studied as an example of subsurface protected niche of astrobiological interest. Different conventional (enrichment and isolation) and molecular ecology techniques (cloning, fluorescence“in situ”probe hybridization, FISH) have been used for isolation and bacterial identification.

Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert

2015 ◽  
Vol 71 (1) ◽  
pp. 150-163 ◽  
Author(s):  
Jin Wang ◽  
Jing-Ting Bao ◽  
Xin-Rong Li ◽  
Yu-Bing Liu
Keyword(s):  
Molecular Ecology ◽  
Tengger Desert ◽  
Nifh Genes ◽  
The Tengger Desert

hyRAD RNA probes preparation and capture v2

2021 ◽  
Author(s):  
Tomasz Suchan ◽  
Ludovic Orlando
Keyword(s):  
Ancient Dna ◽  
Molecular Ecology ◽  
Rna Probes ◽  
Dna Capture

Supplemental Information for: Suchan, T., Kusliy, M.A., Khan, N., Chauvey, L., Tonasso-Calvière, L., Schiavinato, S., Southon, J., Keller, M., Kitagawa, K., Krause, J., Bessudnov, A.N., Bessudnov, A.A., Graphodatsky, A.S., Lamas, S.V., Wilczyński, J., Pospuła, S., Tunia, K., Nowak, M., Moskal-delHoyo, M., Tishkin, A.A., Pryor, A.J.E., Outram, A.K., Orlando, L. (2021) Performance and automation of ancient DNA capture with RNA hyRAD probes. Molecular Ecology Resources, doi: 10.1111/1755-0998.13518

scholarly journals Recent developments in nucleic acid based techniques for use in rumen manipulation

2009 ◽  
Vol 38 (spe) ◽  
pp. 341-351 ◽  
Author(s):  
Christopher McSweeney ◽  
Seungha Kang ◽  
Emma Gagen ◽  
Carl Davis ◽  
Mark Morrison ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nucleic Acid ◽  
Microbial Communities ◽  
Marker Gene ◽  
Molecular Ecology ◽  
Functional Gene ◽  
Rumen Microorganisms ◽  
Dna And Rna ◽  
Conventional Culture ◽  
Reference Strains

Nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation are now being employed regularly in ruminant nutrition studies. Conventional culture-based methods for enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) have been superseded and are now used mainly to obtain pure isolates of novel organisms and reference strains that are required for the development and validation of the nucleic acid approaches. These reference strains are also essential for physiological studies of the lifestyle of the organisms as well as sources of genomic DNA and RNA that can be analysed for functional gene activity. The foundation of the molecular ecology techniques is 16S/18S rDNA sequence analysis which has provided a phylogenetically based classification scheme for enumeration and identification of microbial community members. The use of this marker gene in assays involving the use of single nucleic acid probes or primer sets is rapidly evolving to high throughput approaches such as microarray analysis and new generation sequencing technologies. While these analyses are very informative for determining the composition of the microbial community and monitoring changes in population size, they can only infer function based on these observations. The focus of nucleic acid research is now shifting to the functional analysis of the ecosystem which involves the measurement of functional genes and their expression in the predominant or specific members of the rumen microbial community. Functional gene studies are less developed than 16S rDNA-based analysis of community structure. Also for gene expression studies there are inherent problems involved in extracting high quality RNA from digesta, and priming cDNA synthesis from bacterial mRNA. This paper reviews nucleic acid based molecular methods which have recently been developed for studying the structure and function of rumen microbial communities.

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