BrPARP1, a Poly (ADP-Ribose) Polymerase Gene, Is Involved in Root Development in Brassica rapa under Drought Stress

PARP proteins are highly conserved homologs among the eukaryotic poly (ADP-ribose) polymerases. After activation, ADP-ribose polymers are synthesized on a series of ribozymes that use NAD+ as a substrate. PARPs participate in the regulation of various important biological processes, such as plant growth, development, and stress response. In this study, we characterized the homologue of PARP1 in B. rapa using RNA interference (RNAi) to reveal the underlying mechanism responding to drought stress. Bioinformatics and expression pattern analyses demonstrated that two copy numbers of PARP1 genes (BrPARP1.A03 and BrPARP1.A05) in B. rapa following a whole-genome triplication (WGT) event were retained compared with Arabidopsis, but only BrPARP1.A03 was predominantly transcribed in plant roots. Silencing of BrPARP1 could markedly promote root growth and development, probably via regulating cell division, and the transgenic Brassica lines showed more tolerance under drought treatment, accompanied with substantial alterations including accumulated proline contents, significantly reduced malondialdehyde, and increased antioxidative enzyme activity. In addition, the findings showed that the expression of stress-responsive genes, as well as reactive oxygen species (ROS)-scavenging related genes, was largely reinforced in the transgenic lines under drought stress. In general, these results indicated that BrPARP1 likely responds to drought stress by regulating root growth and the expression of stress-related genes to cope with adverse conditions in B. rapa.

Advancing Endodormancy Release in Temperate Fruit Trees Using Agrochemical Treatments

Endodormancy in temperate fruit trees like Prunus is a protector state that allows the trees to survive in the adverse conditions of autumn and winter. During this process, plants accumulate chill hours. Flower buds require a certain number of chill hours to release from endodormancy, known as chilling requirements. This step is crucial for proper flowering and fruit set, since incomplete fulfillment of the chilling requirements produces asynchronous flowering, resulting in low quality flowers, and fruits. In recent decades, global warming has endangered this chill accumulation. Because of this fact, many agrochemicals have been used to promote endodormancy release. One of the first and most efficient agrochemicals used for this purpose was hydrogen cyanamide. The application of this agrochemical has been found to advance endodormancy release and synchronize flowering time, compressing the flowering period and increasing production in many species, including apple, grapevine, kiwi, and peach. However, some studies have pointed to the toxicity of this agrochemical. Therefore, other non-toxic agrochemicals have been used in recent years. Among them, Erger® + Activ Erger® and Syncron® + NitroActive® have been the most popular alternatives. These two treatments have been shown to efficiently advance endodormancy release in most of the species in which they have been applied. In addition, other less popular agrochemicals have also been applied, but their efficiency is still unclear. In recent years, several studies have focused on the biochemical and genetic variation produced by these treatments, and significant variations have been observed in reactive oxygen species, abscisic acid (ABA), and gibberellin (GA) levels and in the genes responsible for their biosynthesis. Given the importance of this topic, future studies should focus on the discovery and development of new environmentally friendly agrochemicals for improving the modulation of endodormancy release and look more deeply into the effects of these treatments in plants.

Assessment of Aquatic Reed Stands from Airborne Photogrammetric 3K Data

Aquatic reed beds provide important ecological functions, yet their monitoring by remote sensing methods remains challenging. In this study, we propose an approach of assessing aquatic reed stand status indicators based on data from the airborne photogrammetric 3K-system of the German Aerospace Center (DLR). By a Structure from Motion (SfM) approach, we computed stand surface models of aquatic reeds for each of the 14 areas of interest (AOI) investigated at Lake Chiemsee in Bavaria, Germany. Based on reed heights, we subsequently calculated the reed area, surface structure homogeneity and shape of the frontline. For verification, we compared 3K aquatic reed heights against reed stem metrics obtained from ground-based infield data collected at each AOI. The root mean square error (RMSE) for 1358 reference points from the 3K digital surface model and the field-measured data ranged between 39 cm and 104 cm depending on the AOI. Considering strong object movements due to wind and waves, superimposed by water surface effects such as sun glint altering 3K data, the results of the aquatic reed surface reconstruction were promising. Combining the parameter height, area, density and frontline shape, we finally calculated an indicator for status determination: the aquatic reed status index (aRSI), which is based on metrics, and thus is repeatable and transferable in space and time. The findings of our study illustrate that, even under the adverse conditions given by the environment of the aquatic reed, aerial photogrammetry can deliver appropriate results for deriving objective and reconstructable parameters for aquatic reed status (Phragmites australis) assessment.

Turning the Knobs: The Impact of Post-translational Modifications on Carbon Metabolism

Plants rely on the carbon fixed by photosynthesis into sugars to grow and reproduce. However, plants often face non-ideal conditions caused by biotic and abiotic stresses. These constraints impose challenges to managing sugars, the most valuable plant asset. Hence, the precise management of sugars is crucial to avoid starvation under adverse conditions and sustain growth. This review explores the role of post-translational modifications (PTMs) in the modulation of carbon metabolism. PTMs consist of chemical modifications of proteins that change protein properties, including protein-protein interaction preferences, enzymatic activity, stability, and subcellular localization. We provide a holistic view of how PTMs tune resource distribution among different physiological processes to optimize plant fitness.

Recognition of Human Face Regions under Adverse Conditions—Face Masks and Glasses—In Thermographic Sanitary Barriers through Learning Transfer from an Object Detector

The COVID-19 pandemic has detrimentally affected people’s lives and the economies of many countries, causing disruption in the health, education, transport, and other sectors. Several countries have implemented sanitary barriers at airports, bus and train stations, company gates, and other shared spaces to detect patients with viral symptoms in an effort to contain the spread of the disease. As fever is one of the most recurrent disease symptoms, the demand for devices that measure skin (body surface) temperature has increased. The thermal imaging camera, also known as a thermal imager, is one such device used to measure temperature. It employs a technology known as infrared thermography and is a noninvasive, fast, and objective tool. This study employed machine learning transfer using You Only Look Once (YOLO) to detect the hottest temperatures in the regions of interest (ROIs) of the human face in thermographic images, allowing the identification of a febrile state in humans. The algorithms detect areas of interest in the thermographic images, such as the eyes, forehead, and ears, before analyzing the temperatures in these regions. The developed software achieved excellent performance in detecting the established areas of interest, adequately indicating the maximum temperature within each region of interest, and correctly choosing the maximum temperature among them.

Organizational Resilience Capability and Capacity Building

The objective of this chapter is to analyze the implications of organizational resilience capability and capacity building and development processes and the posed challenges to its design and implementation. It is based on the conceptual and theoretical assumptions underpinning the capabilities of resilience that can be learned and designed by organizations to be implemented and applied to adverse conditions. These underlying assumptions affect the organizational resilience capabilities building. It is concluded that building and developing organizational resilience capabilities has increased the research agenda on the theoretical and conceptual literature and the notions, factors, elements, and challenges.

Strategic Adaptive Resilience Capacity

This chapter analyzes the adaptive resilience capacity as an organizational strategy. It is assumed that the development of organizational resilience capabilities can support the transformation and adaptation strategies aimed to enhance the socio ecosystem services. One of the organizational capabilities is organizational resilience assuming that adverse conditions have an impact on the organization which may remain vulnerable unless it learns new capabilities and actions, adapts to access changing resources, and creates iteratively new forms and opportunities with the available resources. It is concluded that a strategic adaptive capacity approach to organizational resilience supports the design and implementation of more flexible and progressive strategies to face any kind of environmental disturbances, crises, and shocks to become more competitive in the global marketplace environment.

Applications of Metagenomics for Unrevealing the Extended Horizons of Microbiota Prevalence from Soil to Human Health

Phylogenetic analysis of different ecosystems has shown that the number of microbial communities in a single sample exceeds their cultured counterparts. Microbes have been found throughout nature and can thrive in adverse conditions. Besides inhabiting diverse environments, they also play a key role in the maintenance of the ecosystem. Most of these microbes are either unculturable or difficult to culture with conventional culturing methods. Metagenomics is an emerging field of science that has been in the light for a decade and offers a potential way to assess microbial diversity. The development of metagenomics opens new ways to study genetic material directly from the environmental samples. DNA sequencing and synthesis technologies are making it possible to read and write entire genomes. The huge amount of data obtained from genome sequencing inevitably requires bioinformatics tools to handle and further process them for analysis. Advances in DNA sequencing and high-performance computing have brought about exemplar improvement in metagenomics, allowing in-depth study of the largely unexplored frontier of microbial life. This culture-independent method provides extensive information regarding the structure, composition, and function of the diverse assemblages of the environmental microbes. The current review presents an overview of the technical aspects of metagenomics along with its diverse applications.

Improvement of the Adaptive Properties of the National Financial Market to Respond the Globalization Risks

In the era of globalization the issues of ensuring economic and national security are still relevant for discussion. Financial markets, being one of the driving forces for the global economic development, are sensitive to changes in the socio-economic and political situations. In the rapidly changing world, the security of the national financial market can be ensured by increasing its adaptability, which is, in the general case, the market ability to adapt to stressful events, while working in adverse conditions. The article discusses the features of the national financial market in the context of financial globalization. Using the complex adaptive theory, the main ways of the national financial market development to enhance its adaptability are presented. Keywords. Globalization, financial market, financial security, financial market adaptability, institutional environment.

To Fight or to Grow: The Balancing Role of Ethylene in Plant Abiotic Stress Responses

Plants often live in adverse environmental conditions and are exposed to various stresses, such as heat, cold, heavy metals, salt, radiation, poor lighting, nutrient deficiency, drought, or flooding. To adapt to unfavorable environments, plants have evolved specialized molecular mechanisms that serve to balance the trade-off between abiotic stress responses and growth. These mechanisms enable plants to continue to develop and reproduce even under adverse conditions. Ethylene, as a key growth regulator, is leveraged by plants to mitigate the negative effects of some of these stresses on plant development and growth. By cooperating with other hormones, such as jasmonic acid (JA), abscisic acid (ABA), brassinosteroids (BR), auxin, gibberellic acid (GA), salicylic acid (SA), and cytokinin (CK), ethylene triggers defense and survival mechanisms thereby coordinating plant growth and development in response to abiotic stresses. This review describes the crosstalk between ethylene and other plant hormones in tipping the balance between plant growth and abiotic stress responses.