Dexmedetomidine protects cardiac microvascular endothelial cells from the damage of OGD/R through regulation of the PPARδ‐mediated autophagy

2020 ◽  
Author(s):  
Qingbo Shao ◽  
Jing Xia ◽  
Pinwen Wu ◽  
Jiazhou Ying
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Involvement of the FoxO3a pathway in the ischemia/reperfusion injury of cardiac microvascular endothelial cells

2013 ◽  
Vol 95 (2) ◽  
pp. 242-247 ◽  
Author(s):  
Xu-Feng Qi ◽  
Yun-Jian Li ◽  
Zhuo-Ying Chen ◽  
Soo-Ki Kim ◽  
Kyu-Jae Lee ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

scholarly journals Acute stimulation of glucose transport by histamine in cardiac microvascular endothelial cells

1995 ◽  
Vol 1268 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Julia Thomas ◽  
Marieke Linssen ◽  
Ger J. van der Vusse ◽  
Barbara Hirsch ◽  
Peter Rösen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Glucose Transport ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells ◽  
Stimulation Of

Isolation and characterization of human and rat cardiac microvascular endothelial cells

1993 ◽  
Vol 264 (2) ◽  
pp. H639-H652 ◽  
Author(s):  
M. Nishida ◽  
W. W. Carley ◽  
M. E. Gerritsen ◽  
O. Ellingsen ◽  
R. A. Kelly ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelium ◽  
Adult Rat ◽  
Isolation And Characterization ◽  
Ventricular Tissue ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Although reciprocal intercellular signaling may occur between endocardial or microvascular endothelium and cardiac myocytes, suitable in vitro models have not been well characterized. In this report, we describe the isolation and primary culture of cardiac microvascular endothelial cells (CMEC) from both adult rat and human ventricular tissue. Differential uptake of fluorescently labeled acetylated low-density lipoprotein (Ac-LDL) indicated that primary isolates of rat CMEC were quite homogeneous, unlike primary isolates of human ventricular tissue, which required cell sorting based on Ac-LDL uptake to create endothelial cell-enriched primary cultures. The endothelial phenotype of both primary isolates and postsort subcultured CMEC and their microvascular origin were determined by characteristic histochemical staining for a number of endothelial cell-specific markers, by the absence of cells with fibroblast or pericyte-specific cell surface antigens, and by rapid tube formation on purified basement membrane preparations. Importantly, [3H]-thymidine uptake was increased 2.3-fold in subconfluent rat microvascular endothelial cells 3 days after coculture with adult rat ventricular myocytes because of release of an endothelial cell mitogen(s) into the extracellular matrix, resulting in a 68% increase in cell number compared with CMEC in monoculture. Thus biologically relevant cell-to-cell interactions can be modeled with this in vitro system.

Autophagy inhibits high glucose induced cardiac microvascular endothelial cells apoptosis by mTOR signal pathway

APOPTOSIS ◽  
2017 ◽  
Vol 22 (12) ◽  
pp. 1510-1523 ◽  
Author(s):  
Zheng Zhang ◽  
Shenwei Zhang ◽  
Yong Wang ◽  
Ming Yang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Signal Pathway ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Basal Nonselective Cation Permeability in Rat Cardiac Microvascular Endothelial Cells

2002 ◽  
Vol 64 (2) ◽  
pp. 187-197 ◽  
Author(s):  
Francesco Moccia ◽  
Roberto Berra-Romani ◽  
Silvana Baruffi ◽  
Santina Spaggiari ◽  
David J. Adams ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Cation Permeability ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Abstract 269: Exendin-4 Prevents Glucose-induced Secretion of Tissue Transglutaminase-2 from Cardiac Microvascular Endothelial Cells: Potential Role in the Prevention of Matrix Remodeling in Diabetes

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Ali S Shihab ◽  
Vanitra A Richardson ◽  
Betsy B Dokken
Keyword(s):  
Endothelial Cells ◽  
Protein Expression ◽  
High Glucose ◽  
Tissue Transglutaminase ◽  
Vascular Complications ◽  
Transglutaminase 2 ◽  
Conditioned Media ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Diabetes causes endothelial dysfunction, which is the initial trigger for vascular complications in diabetic patients. Hyperglycemia initiates a cascade of events that alters protein expression and secretion by endothelial cells. Tissue transglutaminase-2 (tTG2) is an enzyme that under physiologic conditions is sequestered inside the endothelial cell, but under pathologic conditions causing decreased bioavailability of nitric oxide, tTG2 is secreted, activated, and catalyzes irreversible crosslinking of proteins in the extracellular matrix (ECM). Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor (GLP-1R) agonist, used in the treatment of type 2 diabetes, which has vasculo-protective effects. We hypothesized that hyperglycemic stress would induce secretion of tTG2, and that this effect would be attenuated by Ex-4. Mouse cardiac microvascular endothelial cells (MCECs) were serum-starved and exposed to control (5.5 mM glucose) or hyperglycemic (25 mM glucose) conditions, with or without Ex-4 (10 nM) x 72 hrs. Proteins from conditioned media were isolated, trypsinized, and analyzed using LC-MS/MS (LTQ Orbitrap Velos). Immunoblots from cell homogenate were probed for tTG protein expression. Conditioned media from MCECs exposed to high-glucose but not Ex-4 contained tTG2, which was absent in media from cells exposed to high-glucose and Ex-4, as well as in media from control cells, suggesting that Ex-4 prevented the secretion of tTG2 induced by hyperglycemic stress. Protein expression in cell lysate was not different. These findings may have important implications for the etiology of diabetic vascular complications, and for the role of Ex-4 to prevent the pathologic ECM remodeling associated with diabetic vasculopathy. Further studies are ongoing to determine the mechanisms of glucose-induced secretion of tTG2, as well as the mechanisms by which Ex-4 prevents this effect.

Isolation of Mouse Cardiac Microvascular Endothelial Cells

2004 ◽  
pp. 15-22
Author(s):  
Ralf P. Brandes ◽  
Stefani Beer ◽  
Alexandra Aicher
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Scutellarin protects human cardiac microvascular endothelial cells with hypoxia-reoxygenation injury via JAK2/STAT3 signal pathway

2019 ◽  
Vol 11 (1) ◽  
pp. 103-107
Author(s):  
Chen Chen ◽  
Zhi-ying Weng ◽  
You-lan Wang ◽  
Chang-bo Zheng ◽  
Yang Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Signal Pathway ◽  
Microvascular Endothelial Cells ◽  
Reoxygenation Injury ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells ◽  
Hypoxia Reoxygenation
Sign in / Sign up

Export Citation Format

Share Document