Activation of biosynthetic gene clusters by the global transcriptional regulator TRI6 in Fusarium graminearum

2020 ◽  
Vol 114 (4) ◽  
pp. 664-680
Author(s):  
Kristina Shostak ◽  
Christopher Bonner ◽  
Amanda Sproule ◽  
Indira Thapa ◽  
Samuel W. J. Shields ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Transcriptional Regulator ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

scholarly journals DNA Methylation Is Responsive to the Environment and Regulates the Expression of Biosynthetic Gene Clusters, Metabolite Production, and Virulence in Fusarium graminearum

2021 ◽  
Vol 1 ◽  
Author(s):  
Christopher Bonner ◽  
Amanda Sproule ◽  
Owen Rowland ◽  
David Overy ◽  
Rajagopal Subramaniam
Keyword(s):  
Dna Methylation ◽  
Fusarium Graminearum ◽  
External Environment ◽  
Gene Clusters ◽  
Dna Methyltransferases ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Mutant Strains ◽  
Average Methylation ◽  
Methylation Patterns

Histone modifications play a significant role in the regulation of biosynthetic gene clusters (BGCs) in the phytopathogen Fusarium graminearum, by contrast, epigenetic regulation by DNA methyltransferases (DNMTs) is less documented. In this study, we characterized two DNMTs (FgDIM-2 and FgRID) in F. graminearum, with homologies to “Deficient in methylation” (DIM-2) and “Repeat-induced point (RIP) deficient” (RID) from Neurospora. The loss of DNMTs resulted in not only a decrease in average methylation density in the nutrient-poor, compared to nutrient-rich conditions, but also differences in the genes expressed between the WT and the DNMT mutant strains, implicating the external environment as an important trigger in altering DNA methylation patterns. Consequently, we observed significant changes in the regulation of multiple BGCs and alterations in the pathogenicity of the fungus.

Heterologous expression of intact biosynthetic gene clusters in Fusarium graminearum

2019 ◽  
Vol 132 ◽  
pp. 103248 ◽  
Author(s):  
Mikkel Rank Nielsen ◽  
Rasmus Dam Wollenberg ◽  
Klaus Ringsborg Westphal ◽  
Teis Esben Sondergaard ◽  
Reinhard Wimmer ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Fusarium Graminearum ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

2014 ◽  
Vol 77 (12) ◽  
pp. 2619-2625 ◽  
Author(s):  
Jens Laurids Sørensen ◽  
Teis Esben Sondergaard ◽  
Lorenzo Covarelli ◽  
Patricia Romans Fuertes ◽  
Frederik Teilfeldt Hansen ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

scholarly journals Global biogeographic sampling of bacterial secondary metabolism

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Zachary Charlop-Powers ◽  
Jeremy G Owen ◽  
Boojala Vijay B Reddy ◽  
Melinda A Ternei ◽  
Denise O Guimarães ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Genome Sequencing ◽  
Geographic Distance ◽  
Gene Clusters ◽  
Natural World ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Road Map ◽  
Two Factors ◽  
Natural Products Discovery

Recent bacterial (meta)genome sequencing efforts suggest the existence of an enormous untapped reservoir of natural-product-encoding biosynthetic gene clusters in the environment. Here we use the pyro-sequencing of PCR amplicons derived from both nonribosomal peptide adenylation domains and polyketide ketosynthase domains to compare biosynthetic diversity in soil microbiomes from around the globe. We see large differences in domain populations from all except the most proximal and biome-similar samples, suggesting that most microbiomes will encode largely distinct collections of bacterial secondary metabolites. Our data indicate a correlation between two factors, geographic distance and biome-type, and the biosynthetic diversity found in soil environments. By assigning reads to known gene clusters we identify hotspots of biomedically relevant biosynthetic diversity. These observations not only provide new insights into the natural world, they also provide a road map for guiding future natural products discovery efforts.

Biosynthetic strategies for tetramic acid formation

2021 ◽  
Author(s):  
Xuhua Mo ◽  
Tobias A. M. Gulder
Keyword(s):  
Molecular Mechanism ◽  
Gene Clusters ◽  
Acid Formation ◽  
Biosynthetic Gene ◽  
Tetramic Acid ◽  
Biosynthetic Gene Clusters ◽  
The Individual ◽  
First Time ◽  
Acid Unit

Over 30 biosynthetic gene clusters for natural tetramate have been identified. This highlight reviews the biosynthetic strategies for formation of tetramic acid unit for the first time, discussing the individual molecular mechanism in detail.

Challenges of functional expression of complex polyketide biosynthetic gene clusters

2021 ◽  
Vol 69 ◽  
pp. 103-111
Author(s):  
Yaojie Gao ◽  
Yuchun Zhao ◽  
Xinyi He ◽  
Zixin Deng ◽  
Ming Jiang
Keyword(s):  
Gene Clusters ◽  
Functional Expression ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters
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