scholarly journals Mining RNA‐seq data reveals the massive regulon of GcvB small RNA and its physiological significance in maintaining amino acid homeostasis in Escherichia coli

2021 ◽  
Author(s):  
Masatoshi Miyakoshi ◽  
Haruna Okayama ◽  
Maxence Lejars ◽  
Takeshi Kanda ◽  
Yuki Tanaka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Small Rna ◽  
Physiological Significance ◽  
Rna Seq ◽  
Amino Acid Homeostasis

Involvement of GcvB small RNA in intrinsic resistance to multiple aminoglycoside antibiotics in Escherichia coli

2020 ◽  
Author(s):  
Akira Muto ◽  
Simon Goto ◽  
Daisuke Kurita ◽  
Chisato Ushida ◽  
Hyota Himeno
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Small Rna ◽  
Antibiotic Sensitivity ◽  
Enteric Bacteria ◽  
Aminoglycoside Antibiotics ◽  
Amino Acid Transporters ◽  
Intrinsic Resistance ◽  
Amino Acid Incorporation ◽  
Low Concentrations

Abstract Deleting the gene for small RNA GcvB in Escherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the expression of many genes for amino acid incorporation systems, especially the periplasmic ABC-transporter proteins. Deletions of several amino acid transporter genes in ΔgcvB cells decreased the antibiotic sensitivity to the wild-type level, suggesting that those genes are involved in uptake of aminoglycosides into the cell. Since GcvB is constitutively synthesized in growing cells, repressing synthesis of amino acid transporters, it contributes to the intrinsic resistance to several aminoglycoside antibiotics.

scholarly journals Amino acid substitutions and alteration in cation specificity in the melibiose carrier of Escherichia coli.

1988 ◽  
Vol 263 (28) ◽  
pp. 14276-14280 ◽  
Author(s):  
T Kawakami ◽  
Y Akizawa ◽  
T Ishikawa ◽  
T Shimamoto ◽  
M Tsuda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Substitutions
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