scholarly journals The eukaryotic protein kinase superfamily of the necrotrophic fungal plant pathogen,Sclerotinia sclerotiorum

2015 ◽  
Vol 17 (4) ◽  
pp. 634-647 ◽  
Author(s):  
Dwayne D. Hegedus ◽  
Kelsey Gerbrandt ◽  
Cathy Coutu
Keyword(s):  
Protein Kinase ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Eukaryotic Protein Kinase ◽  
Eukaryotic Protein ◽  
Fungal Plant Pathogen

The Eukaryotic Protein Kinase Domain

2005 ◽  
pp. 181-209 ◽  
Author(s):  
Arvin C. Dar ◽  
Leanne E. Wybenga-Groot ◽  
Frank Sicheri
Keyword(s):  
Protein Kinase ◽  
Kinase Domain ◽  
Protein Kinase Domain ◽  
Eukaryotic Protein Kinase ◽  
Eukaryotic Protein

scholarly journals Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2 as a Representative Example

2011 ◽  
pp. 359-368 ◽  
Author(s):  
Andrea Piserchio ◽  
Kevin N. Dalby ◽  
Ranajeet Ghose
Keyword(s):  
Protein Kinase ◽  
Eukaryotic Protein Kinase ◽  
Eukaryotic Protein

scholarly journals Screening of Microbial Extracts for Anticancer Compounds Using Streptomyces Kinase Inhibitor Assay

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Prashant Shanbhag ◽  
Sarita Bhave ◽  
Ashwini Vartak ◽  
Asha Kulkarni-Almeida ◽  
Girish Mahajan ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Anticancer Agents ◽  
Kinase Inhibitors ◽  
Kinase Inhibitor ◽  
Protein Kinase Inhibitors ◽  
Kinase Assay ◽  
Diffusion Method ◽  
Eukaryotic Protein Kinase ◽  
Eukaryotic Protein ◽  
Microbial Extracts

Eukaryotic kinases are known to play an important role in signal transduction pathways by phosphorylating their respective substrates. Abnormal phosphorylations by these kinases have resulted in diseases. Hence inhibitors of kinases are of considerable pharmaceutical interest for a wide variety of disease targets, especially cancers. A number of reports have been published which indicate that eukaryotic-like kinases may complement two-component kinase systems in several bacteria. In Streptomyces sp. such kinases have been found to have a role in formation of aerial hyphae, spores, pigmentation & even in antibiotic production in some strains. Eukaryotic kinase inhibitors are seen to inhibit formation of aerial mycelia in Streptomyces without inhibiting vegetative mycelia. This property has been used to design an assay to screen for eukaryotic kinase inhibitors. The assay involves testing of compounds against Streptomyces 85E ATCC 55824 using agar well diffusion method. Inhibitors of kinases give rise to “bald” colonies where aerial mycelia and sporulation inhibition is seen. The assay has been standardized using known eukaryotic protein kinase inhibiting anticancer agents like AG-490, AG-1295, AG-1478, Flavopiridol and Imatinib as positive controls, at a concentration ranging from 10 μg/well to 100 μg/well. Anti-infective compounds which are not reported to inhibit eukaryotic protein kinases were used as negative controls. A number of microbial cultures have been screened for novel eukaryotic protein kinase inhibitors. Further these microbial extracts were tested in various cancer cell lines like Panc1, HCT116, Calu1, ACHN and H460 at a concentration of 10 μg/mL/ well. The anticancer data was seen correlating well with the Streptomyces kinase assay thus validating the assay.

A novel alphahypovirus that infects the fungal plant pathogen Sclerotinia sclerotiorum

2021 ◽  
Author(s):  
Weibo Liang ◽  
Zhongbo Lu ◽  
Jie Duan ◽  
Daohong Jiang ◽  
Jiatao Xie ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Fungal Plant Pathogen

scholarly journals Purification of two Bacillus subtilis proteins which cross-react with antibodies directed against eukaryotic protein kinase C, the His HPr kinase and trigger factor

Microbiology ◽  
1997 ◽  
Vol 143 (4) ◽  
pp. 1151-1161 ◽  
Author(s):  
N. Zouari ◽  
B. Roche ◽  
J. F. M. L. Seegers ◽  
S. J. Seror
Keyword(s):  
Bacillus Subtilis ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Trigger Factor ◽  
Eukaryotic Protein Kinase ◽  
Eukaryotic Protein ◽  
Kinase C

scholarly journals Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 336 ◽  
Author(s):  
Koanna Guyon ◽  
Claudine Balagué ◽  
Dominique Roby ◽  
Sylvain Raffaele
Keyword(s):  
Host Range ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Broad Host Range ◽  
Secretome Analysis ◽  
Fungal Plant Pathogen
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