The human translation of the postinfectious irritable bowel syndrome like rat model with antivinculin production after immunization with cytolethal distending toxin B

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Max Schmulson ◽  
María Fernanda Davalos De la Rosa ◽  
Laura Linares‐Garcia ◽  
Alizon Sujey Morales‐Guzmán ◽  
Rita Balbuena ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Rat Model ◽  
Cytolethal Distending Toxin ◽  
Toxin B ◽  
Irritable Bowel ◽  
Postinfectious Irritable Bowel Syndrome

scholarly journals Study of Antibodies to Cytolethal Distending Toxin B (CdtB) and Antibodies to Vinculin in Patients with Irritable Bowel Syndrome

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 303
Author(s):  
Maysaa El Sayed Zaki ◽  
Dina Elhammady ◽  
Mona Foda Salama ◽  
Mostafa Abdelsalam ◽  
Asmaa Osama Bakr Osman
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gastrointestinal Disorder ◽  
The Other ◽  
University Hospital ◽  
Cytolethal Distending Toxin ◽  
Retrospective Case ◽  
Toxin B ◽  
Control Subjects ◽  
Irritable Bowel ◽  
Post Infectious

Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder, categorized into various subtypes. Post-infection IBS may be attributed to the release of cytolethal distending toxin B (CdtB), which cross-reacts with the adhesion protein vinculin responsible for normal intestinal contractility. Objective: This study aims to identify anti-CdtB and anti-vinculin levels in IBS patients compared to healthy control. Subjects and methods: This retrospective case-control study was conducted on 100 patients with IBS, as determined by a questionnaire based on Rome IV criteria, recruited from the outpatient clinics of the Tropical Medicine at Mansoura University Hospital from January 2019 to January 2020. Results: Anti-vinculin and anti-CdtB levels were significantly elevated in patients with IBS (1.58±0.496ng/ml, 2.47±0.60ng/ml)  when compared to control subjects (1.13±0.249ng/ml, 2.1±0.24 ng/ml), respectively with P=0.001 for both.  Anti-vinculin level was significantly higher in the IBS-D subtype than the other subtypes (P=0.001) while, Anti-CdtB was significantly elevated in IBS-C, IBS-D subgroups compared to control subjects (P=0.001). Conclusion: Findings of the present study support the hypothesis that IBS results from post-infectious disorders initiated by bacterial enteritis. A hypothesis could be applied to all IBS subgroups. On the other hand. These biomarkers might reflect the post-infectious state's severity. These findings need further extensive longitudinal studies in patients with IBS.

scholarly journals Electroacupuncture Relieves Visceral Hypersensitivity by Inactivating Protease-Activated Receptor 2 in a Rat Model of Postinfectious Irritable Bowel Syndrome

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Wanli Xu ◽  
Mengqian Yuan ◽  
Xiaoliang Wu ◽  
Hao Geng ◽  
Lu Chen ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Rat Model ◽  
Analgesic Effect ◽  
Molecular Mechanisms ◽  
Critical Role ◽  
Visceral Hypersensitivity ◽  
Mrna Levels ◽  
Irritable Bowel ◽  
Protease Activated Receptor 2 ◽  
Postinfectious Irritable Bowel Syndrome

Background. The role of protease-activated receptor 2 (PAR2) in the analgesic effect of electroacupuncture (EA) on visceral hypersensitivity (VH) in postinfectious irritable bowel syndrome (PI-IBS) has yet to be elucidated. Aim. In this study, we investigated the molecular mechanisms underlying the analgesic effect of EA in a rat model of PI-IBS. Methods. Visceral hypersensitivity was evaluated by the abdominal withdrawal reflex test before and after administration of the PAR2 agonist, PAR2-AP, and/or EA. The protein expression and mRNA levels of PAR2, CGRP, SP, and TPSP in colon tissues were measured by immunofluorescence, western blot, and RT-PCR. Results. We found that EA could alleviate VH and significantly decrease protein and mRNA levels of PAR2, TPSP, CGRP, and SP in PI-IBS rats. The analgesic effect of EA on VH was slightly reduced in the presence of PAR2-AP. Conclusions. These results suggest that EA alleviates VH symptoms through downregulation of the levels of the TPSP/PAR2/SP/CGRP signaling axis in colon tissues in PI-IBS rats. Together, our data suggests that PAR2 plays a critical role in the analgesic effect of EA on VH in PI-IBS.

scholarly journals Study of Antibodies to Cytolethal Distending Toxin B (CdtB) and Antibodies to Vinculin in Patients with Irritable Bowel Syndrome

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 303
Author(s):  
Maysaa El Sayed Zaki ◽  
Dina Elhammady ◽  
Mona Foda Salama ◽  
Mostafa Abdelsalam ◽  
Asmaa Osama Bakr Osman
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gastrointestinal Disorder ◽  
The Other ◽  
University Hospital ◽  
Cytolethal Distending Toxin ◽  
Retrospective Case ◽  
Toxin B ◽  
Control Subjects ◽  
Irritable Bowel ◽  
Post Infectious

Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder, categorized into various subtypes. Post-infection IBS may be attributed to the release of cytolethal distending toxin B (CdtB), which cross-reacts with the adhesion protein vinculin responsible for normal intestinal contractility. Objective: This study aims to identify anti-CdtB and anti-vinculin levels in IBS patients compared to healthy control. Subjects and methods: This retrospective case-control study was conducted on 100 subjects with IBS, as determined by a questionnaire based on Rome IV criteria, recruited from the outpatient clinics of the Tropical Medicine at Mansoura University Hospital from January 2019 to January 2020. Results: Anti-vinculin and anti-CdtB levels were significantly elevated in patients with IBS (1.58±0.496ng/ml, 2.47±0.60ng/ml)  when compared to control subjects (1.13±0.249ng/ml, 2.1±0.24 ng/ml), respectively with P=0.001 for both.  Anti-vinculin level was significantly higher in the IBS-D subtype than the other subtypes (P=0.001) while, Anti-CdtB was significantly elevated in IBS-C, IBS-D subgroups compared to control subjects (P=0.001). Conclusion: Findings of the present study support the hypothesis that IBS results from post-infectious disorders initiated by bacterial enteritis. A hypothesis could be applied to all IBS subgroups. On the other hand. These biomarkers might reflect the post-infectious state's severity. These findings need further extensive longitudinal studies in patients with IBS.

Assessment of Anti-vinculin and Anti-cytolethal Distending Toxin B Antibodies in Subtypes of Irritable Bowel Syndrome

2017 ◽  
Vol 62 (6) ◽  
pp. 1480-1485 ◽  
Author(s):  
Ali Rezaie ◽  
Sung Chul Park ◽  
Walter Morales ◽  
Emily Marsh ◽  
Anthony Lembo ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cytolethal Distending Toxin ◽  
Toxin B ◽  
Irritable Bowel

scholarly journals Study of Antibodies to Cytolethal Distending Toxin B (CdtB) and Antibodies to Vinculin in Patients with Irritable Bowel Syndrome

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 303
Author(s):  
Maysaa El Sayed Zaki ◽  
Dina Elhammady ◽  
Mona Foda Salama ◽  
Mostafa Abdelsalam ◽  
Asmaa Osama Bakr Osman
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gastrointestinal Disorder ◽  
The Other ◽  
University Hospital ◽  
Cytolethal Distending Toxin ◽  
Retrospective Case ◽  
Toxin B ◽  
Control Subjects ◽  
Irritable Bowel ◽  
Post Infectious

Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder, categorized into various subtypes. Post-infection IBS may be attributed to the release of cytolethal distending toxin B (CdtB), which cross-reacts with the adhesion protein vinculin responsible for normal intestinal contractility. Objective: This study aims to identify anti-CdtB and anti-vinculin levels in IBS patients compared to healthy control. Subjects and methods: This retrospective case-control study was conducted on 100 subjects with IBS, as determined by a questionnaire based on Rome III criteria, recruited from the outpatient clinics of the Tropical Medicine at Mansoura University Hospital from January 2019 to January 2020. Results: Anti-vinculin and anti-CdtB levels were significantly elevated in patients with IBS (1.58±0.496, 2.47±0.60)  when compared to control subjects (1.13±0.249ng/ml, 2.1±0.24 ng/ml), respectively with P=0.001 for both.  Anti-vinculin level was significantly higher in the IBS-D subtype than the other subtypes (P=0.001) while, Anti-CdtB was significantly elevated in IBS-C, IBS-D subgroups compared to control subjects (P=0.001). Conclusion: Findings of the present study support the hypothesis that IBS results from post-infectious disorders initiated by bacterial enteritis. A hypothesis could be applied to all IBS subgroups. On the other hand. These biomarkers might reflect the post-infectious state's severity.

scholarly journals Study of Antibodies to Cytolethal Distending Toxin B (CdtB) and Antibodies to Vinculin in Patients with Irritable Bowel Syndrome

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 303
Author(s):  
Maysaa El Sayed Zaki ◽  
Dina Elhammady ◽  
Mona Foda Salama ◽  
Mostafa Abdelsalam ◽  
Asmaa Osama Bakr Osman
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gastrointestinal Disorder ◽  
The Other ◽  
University Hospital ◽  
Cytolethal Distending Toxin ◽  
Retrospective Case ◽  
Toxin B ◽  
Control Subjects ◽  
Irritable Bowel ◽  
Post Infectious

Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder, categorized into various subtypes. Post-infection IBS may be attributed to the release of cytolethal distending toxin B (CdtB), which cross-reacts with the adhesion protein vinculin responsible for normal intestinal contractility. Objective: This study aims to identify anti-CdtB and anti-vinculin levels in IBS patients compared to healthy control. Subjects and methods: This retrospective case-control study was conducted on 100 subjects with IBS, as determined by a questionnaire based on Rome III criteria, recruited from the outpatient clinics of the Tropical Medicine at Mansoura University Hospital from January 2019 to January 2020. Results: The optical density (OD) results of the anti-vinculin and anti-CdtB levels were significantly elevated in patients with IBS (1.58±0.496 OD, 2.47±0.60 OD)  when compared to control subjects (1.13±0.249 OD, 2.1±0.24 OD), respectively with P=0.001 for both.  Anti-vinculin level was significantly higher in the IBS-D subtype than the other subtypes (P=0.001) while, Anti-CdtB was significantly elevated in IBS-C, IBS-D subgroups compared to control subjects (P=0.001). Conclusion: Findings of the present study support the hypothesis that IBS results from post-infectious disorders initiated by bacterial enteritis. A hypothesis could be applied to all IBS subgroups. On the other hand. These biomarkers might reflect the post-infectious state's severity.

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