scholarly journals Metabolic profiling of wheat rachis node infection by Fusarium graminearum - decoding deoxynivalenol-dependent susceptibility

2018 ◽  
Vol 221 (1) ◽  
pp. 459-469 ◽  
Author(s):  
Jakob Bönnighausen ◽  
Nicolas Schauer ◽  
Wilhelm Schäfer ◽  
Jörg Bormann
Keyword(s):  
Fusarium Graminearum ◽  
Metabolic Profiling ◽  
Rachis Node

scholarly journals Developing Kernel and Rachis Node Induce the Trichothecene Pathway of Fusarium graminearum During Wheat Head Infection

2009 ◽  
Vol 22 (8) ◽  
pp. 899-908 ◽  
Author(s):  
Peter Ilgen ◽  
Birgit Hadeler ◽  
Frank J. Maier ◽  
Wilhelm Schäfer
Keyword(s):  
Fusarium Graminearum ◽  
Green Fluorescence Protein ◽  
Head Blight ◽  
Plant Infection ◽  
Trichodiene Synthase ◽  
Gene Coding ◽  
In The Wild ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Dsred Gene ◽  
Rachis Node

The fungal pathogen Fusarium graminearum is the most common agent of Fusarium head blight (FHB) in small grain cereals and cob rot of maize. The threat posed by this fungus is due to a decrease in yield and, additionally, mycotoxin contamination of the harvested cereals. Among the mycotoxins, trichothecenes influence virulence of F. graminearum in a highly complex manner that is strongly host- as well as chemotype-specific. The factors inducing mycotoxin production during plant infection are still unknown. To evaluate the induction of the trichothecene pathway, the green fluorescence protein (GFP) gene was fused to the promoter of the TRI5 gene coding for the trichodiene synthase and integrated into the genome by homologous integration. The resulting mutant contains a fully functional TRI5 gene ensuring virulence on wheat and exhibits GFP driven by the endogenous TRI5 promoter. We are now able to monitor the induction of trichothecenes under real-time conditions. To localize the fungus in the plant tissue, the dsRed gene was integrated under constitutive control of the glycerol-3-phosphate dehydrogenase (gpdA) promoter. We are now able to show that, first, induction of GFP as well as trichothecene production in the reporter strain reflects TRI5 induction and trichothecene production in the wild type; second, expression of TRI5 is inducible during growth in culture; and, third, trichothecene production is not uniformly induced during the onset of infection but is tissue specific during fungal infection of wheat.

Metabolic Profiling

2014 ◽  
Keyword(s):  
Metabolic Profiling

Differentiation of Thymus vulgaris L. sample lots by NMR based metabolic profiling

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
V Pieri ◽  
S Sturm ◽  
C Seger ◽  
H Stuppner
Keyword(s):  
Metabolic Profiling ◽  
Thymus Vulgaris

Metabolic Profiling of Maca (Lepidium meyenii) Cultivars using NMR and Multivariate Data Analysis

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
J Zhao ◽  
B Avula ◽  
C Clément ◽  
M Kreuzera ◽  
IA Khan
Keyword(s):  
Data Analysis ◽  
Metabolic Profiling ◽  
Multivariate Data Analysis ◽  
Multivariate Data ◽  
Lepidium Meyenii

Metabolic profiling and antioxidant activity of Sideritis species growing in southeast europe

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
G Papaefstathiou ◽  
N Aligiannis ◽  
N Fokialakis ◽  
L Skaltsounis
Keyword(s):  
Antioxidant Activity ◽  
Metabolic Profiling ◽  
Southeast Europe

Metabolic profiling of the Traditional Chinese formula Fang Feng Tong Sheng San by GC-MS

2017 ◽  
Author(s):  
A Brantner ◽  
Y Zhao ◽  
M Alajlani ◽  
H Zhou ◽  
B Baolin
Keyword(s):  
Metabolic Profiling

Detoxification of zearalenone produced by Fusarium graminearum by purified tannase from Citrobacter freundii

2015 ◽  
Vol 2 (1) ◽  
pp. 001-008
Author(s):  
Nsayef Muslim Sahira ◽  
◽  
N. Mahammed Alaa ◽  
M.S. AL_Kadmy Israa ◽  
Nsayef Muslim Sraa
Keyword(s):  
Fusarium Graminearum ◽  
Citrobacter Freundii
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