Direct Modification of Multiple Gene Homoeologs in Brassica oleracea and Brassica napus Using Doubled Haploid Inducer‐Mediated Genome Editing System

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

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Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.01280 ◽

2010 ◽

Vol 36 (8) ◽

pp. 1280-1285 ◽

Cited By ~ 1

Author(s):

Jun LI ◽

Li-Xia LUO ◽

Zhuan WANG ◽

Jun LI ◽

Kun-Rong CHEN ◽

...

Keyword(s):

Brassica Napus ◽

Brassica Oleracea ◽

Brassica Rapa

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Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

Canadian Journal of Plant Science ◽

10.4141/cjps95-072 ◽

1995 ◽

Vol 75 (2) ◽

pp. 437-439 ◽

Cited By ~ 12

Author(s):

G. R. Stringam ◽

V. K. Bansal ◽

M. R. Thiagarajah ◽

D. F. Degenhardt ◽

J. P. Tewari

Keyword(s):

Brassica Napus ◽

Doubled Haploid ◽

Leptosphaeria Maculans ◽

Resistance Breeding ◽

Breeding Method ◽

Brassica Napus L ◽

Breeding Lines ◽

University Of Alberta ◽

Blackleg Resistance ◽

The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

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Detection and Effects of a Homeologous Reciprocal Transposition in Brassica napus

Genetics ◽

10.1093/genetics/165.3.1569 ◽

2003 ◽

Vol 165 (3) ◽

pp. 1569-1577

Author(s):

Thomas C Osborn ◽

David V Butrulle ◽

Andrew G Sharpe ◽

Kathryn J Pickering ◽

Isobel A P Parkin ◽

...

Keyword(s):

Brassica Napus ◽

Genetic Mapping ◽

Doubled Haploid ◽

Selective Advantage ◽

F1 Hybrids ◽

Rflp Markers ◽

Cytological Analysis ◽

Linkage Groups ◽

Homeologous Chromosomes ◽

Seed Yields

Abstract A reciprocal chromosomal transposition was identified in several annual oilseed Brassica napus genotypes used as parents in crosses to biennial genotypes for genetic mapping studies. The transposition involved an exchange of interstitial homeologous regions on linkage groups N7 and N16, and its detection was made possible by the use of segregating populations of doubled haploid lines and codominant RFLP markers. RFLP probes detected pairs of homeologous loci on N7 and N16 for which the annual and biennial parents had identical alleles in regions expected to be homeologous. The existence of an interstitial reciprocal transposition was confirmed by cytological analysis of synaptonemal complexes of annual × biennial F1 hybrids. Although it included approximately one-third of the physical length of the N7 and N16 chromosomes, few recombination events within the region were recovered in the progenies of the hybrids. Significantly higher seed yields were associated with the parental configurations of the rearrangement in segregating progenies. These progenies contained complete complements of homeologous chromosomes from the diploid progenitors of B. napus, and thus their higher seed yields provide evidence for the selective advantage of allopolyploidy through the fixation of intergenomic heterozygosity.

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Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

Israel Journal of Plant Sciences ◽

10.1163/22238980-bja10020 ◽

2021 ◽

pp. 1-10

Author(s):

Sourbh Kumar ◽

Uttam Chandel ◽

Satish Kumar Guleria

Keyword(s):

Doubled Haploid ◽

Seed Set ◽

Chromosome Doubling ◽

Pollen Viability ◽

Haploid Inducer ◽

Haploid Production ◽

Maize Genotypes ◽

Different Doses

Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

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R1-nj expression in parental inbreds as a predictor of amenability of maize hybrids to R1-nj-based doubled haploid development

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.4.5 ◽

2020 ◽

Vol 79 (04) ◽

Author(s):

R. K. Khulbe ◽

A. Pattanayak ◽

Vivek Panday

Keyword(s):

Doubled Haploid ◽

Current Method ◽

Distinct Pattern ◽

Complex Nature ◽

Maize Breeding ◽

Early Maturity ◽

Haploid Inducer ◽

Source Populations ◽

Phenotype Expression

The current method of doubled haploid (DH) development in maize involves in vivo production of haploids using R1-njbased haploid inducer lines that upon use as male render a small fraction of seed in the pollinated female ears haploid. Identification of haploid seed relies on R1-nj marker expression in the endosperm and embryo, and the degree of its expression determines efficiency of DH development process. In the present study, R1-nj expression in the endosperm was characterized in crosses of CIMMYT’s R1-nj-based haploid inducer TAILP1 with a set comprising 18 early maturity hybrids and their 23 parental inbreds. Kernel colour inhibition was observed only in a small proportion of the hybrids and inbreds. Comparison of R1-nj expression in the hybrids and their parental inbreds revealed a distinct pattern, which may be useful in identifying source populations and/or determining parental constituents for synthesizing source populations with predicted amenability to doubled haploid development using R1-nj-based haploid inducers. However, deviation from the pattern was noted in hybrids involving inbreds with higher degree of colour inhibition, which suggests complex nature of R1-nj phenotype expression and necessitates further investigation involving larger sets of germplasm for dissecting the role of maternal and paternal genetic factors in determining R1-nj phenotype expression. The hybrids found exhibiting complete kernel anthocyanin expression in present study can be used directly as source populations for DH development using R1-nj based haploid inducers. Besides, since the inbreds used in the study have originated from and/or are accessible to CGIAR/NARS maize breeding programmes, the information on their kernel anthocyanin expression can be helpful in selection of source populations or generating new source populations amenable for DH development using R1-nj based haploid inducers.

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