scholarly journals Calm on the surface, dynamic on the inside. Molecular homeostasis of Anabaena sp. PCC 7120 nitrogen metabolism

2021 ◽  
Author(s):  
Giorgio Perin ◽  
Tyler Fletcher ◽  
Virag Sagi‐Kiss ◽  
David C. A. Gaboriau ◽  
Mathew R. Carey ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Anabaena Sp ◽  
Pcc 7120 ◽  
Surface Dynamic

scholarly journals The small Ca2+-binding protein CSE links Ca2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. PCC 7120

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Julia Walter ◽  
Francisco Leganés ◽  
Eva-Mari Aro ◽  
Peter J. Gollan
Keyword(s):  
Nitrogen Metabolism ◽  
Binding Protein ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals PII Is Important in Regulation of Nitrogen Metabolism but Not Required for Heterocyst Formation in the Cyanobacterium Anabaena sp. PCC 7120

2007 ◽  
Vol 282 (46) ◽  
pp. 33641-33648 ◽  
Author(s):  
Ying Zhang ◽  
Hai Pu ◽  
Qingsong Wang ◽  
Shu Cheng ◽  
Weixing Zhao ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrate Uptake ◽  
Nitrogenase Activity ◽  
Tyrosine Nitration ◽  
Wild Type ◽  
Cyanobacterium Anabaena ◽  
In The Wild ◽  
Anabaena Sp ◽  
Key Residues ◽  
Pcc 7120

PII is an important signal protein for regulation of nitrogen metabolism in bacteria and plants. We constructed a mutant of glnB, encoding PII, in a heterocystous cyanobacterium, Anabaena sp. PCC 7120, with a cre-loxP system. The mutant (MP2α) grew more slowly than the wild type under all nitrogen regimens. It excreted a large amount of ammonium when grown on nitrate due to altered activities of glutamine synthetase and nitrate reductase. MP2α had a low nitrogenase activity but was able to form heterocysts under diazotrophic conditions, suggesting that PII is not required for heterocyst differentiation. Analysis of the PII with mass spectroscopy found tyrosine nitration at Tyr-51 under diazotrophic conditions while no phosphorylation at Ser-49 was detected. The strains 51F and 49A, which have PII with mutations of Y51F and S49A, respectively, were constructed to analyze the functions of the two key residues on the T-loop. Like MP2α, they had low nitrogenase activity and grew slowly under diazotrophic conditions. 49A was also impaired in nitrate uptake and formed heterocysts in the presence of nitrate. The up-regulation of ntcA after nitrogen step-down, which was present in the wild type, was not observed in 51F and 49A. While our results showed that the Ser-49 residue is important to the function of PII in Anabaena sp. PCC 7120, evidence from the PII pattern of the wild type and 49A in non-denaturing gel electrophoresis suggested that Ser-49 is not modified. The possible physiological roles of tyrosine nitration of PII are discussed.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

The developmental regulator PatD modulates assembly of the cell‐division protein FtsZ in the cyanobacterium Anabaena sp. PCC 7120

2021 ◽  
Author(s):  
Li Wang ◽  
Tian‐Cai Niu ◽  
Ana Valladares ◽  
Gui‐Ming Lin ◽  
Ju‐Yuan Zhang ◽  
...  
Keyword(s):  
Cell Division ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Cell Division Protein ◽  
Pcc 7120

scholarly journals 2‐oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120

FEBS Letters ◽  
2019 ◽  
Vol 594 (2) ◽  
pp. 278-289
Author(s):  
Jorge Guío ◽  
Cristina Sarasa‐Buisan ◽  
Adrián Velázquez‐Campoy ◽  
María Teresa Bes ◽  
María F. Fillat ◽  
...  
Keyword(s):  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

2011 ◽  
Vol 19 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Guoce YU ◽  
Dingji SHI ◽  
Zhaoling CAI ◽  
Wei CONG ◽  
Fan OUYANG
Keyword(s):  
Mixotrophic Culture ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Cloning, sequencing and transcriptional studies of the genes for cytochrome c-553 and plastocyanin from Anabaena sp. PCC 7120

Microbiology ◽  
1994 ◽  
Vol 140 (5) ◽  
pp. 1151-1159 ◽  
Author(s):  
M. Ghassemian ◽  
B. Wong ◽  
F. Ferreira ◽  
J. L. Markley ◽  
N. A. Straus
Keyword(s):  
Cytochrome C ◽  
Anabaena Sp ◽  
Pcc 7120
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