Light-Induced Conformational Changes in the Plant Cryptochrome Photolyase Homology Region Resolved by Selective Isotope Labeling and Infrared Spectroscopy

2017 ◽  
Vol 93 (3) ◽  
pp. 881-887 ◽  
Author(s):  
Constanze Sommer ◽  
Marina S. Dietz ◽  
Thomas Patschkowski ◽  
Tilo Mathes ◽  
Tilman Kottke
Keyword(s):  
Infrared Spectroscopy ◽  
Conformational Changes ◽  
Isotope Labeling ◽  
Homology Region ◽  
Selective Isotope Labeling

scholarly journals Conformational Changes Generated in GroEL during ATP Hydrolysis as Seen by Time-resolved Infrared Spectroscopy

1999 ◽  
Vol 274 (9) ◽  
pp. 5508-5513 ◽  
Author(s):  
Frithjof von Germar ◽  
Asier Galán ◽  
Oscar Llorca ◽  
Jose L. Carrascosa ◽  
Jose M. Valpuesta ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Conformational Changes ◽  
Atp Hydrolysis ◽  
Time Resolved ◽  
Time Resolved Infrared Spectroscopy

Limited Proteolysis Combined with Stable Isotope Labeling Reveals Conformational Changes in Protein (Pseudo)kinases upon Binding Small Molecules

2015 ◽  
Vol 14 (10) ◽  
pp. 4179-4193 ◽  
Author(s):  
Michela Di Michele ◽  
Elisabeth Stes ◽  
Elien Vandermarliere ◽  
Rohit Arora ◽  
Juan Astorga-Wells ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Small Molecules ◽  
Conformational Changes ◽  
Isotope Labeling ◽  
Limited Proteolysis ◽  
Stable Isotope Labeling

scholarly journals Selective Isotope Labeling to Facilitate Structural and Dynamics Studies of RNAs by NMR Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 356a
Author(s):  
Lukasz T. Olenginski ◽  
Owen Becette ◽  
Hyeyeon Nam ◽  
Kehinde M. Taiwo ◽  
Theodore K. Dayie
Keyword(s):  
Nmr Spectroscopy ◽  
Isotope Labeling ◽  
Selective Isotope Labeling

One-step amino acid selective isotope labeling of proteins in prototrophic Escherichia coli strains

2012 ◽  
Vol 426 (2) ◽  
pp. 126-128 ◽  
Author(s):  
Christopher O’Grady ◽  
Benjamin L. Rempel ◽  
Akosiererem Sokaribo ◽  
Sergiy Nokhrin ◽  
Oleg Y. Dmitriev
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Isotope Labeling ◽  
Selective Isotope Labeling ◽  
One Step

A novel method for selective isotope labeling of bacterially expressed proteins

1995 ◽  
Vol 5 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Karen M. Lee ◽  
Elliot J. Androphy ◽  
James D. Baleja
Keyword(s):  
Isotope Labeling ◽  
Selective Isotope Labeling ◽  
Novel Method

CORNEAL TOXICITY ASSOCIATED WITH AMMONIA EXPOSURE INVESTIGATED BY FOURIER TRANSFORM INFRARED SPECTROSCOPY

2009 ◽  
Vol 04 (04) ◽  
pp. 331-343 ◽  
Author(s):  
SHERIF SIDDICK MAHMOUD
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Conformational Changes ◽  
Biological Effects ◽  
Exothermic Reaction ◽  
Fourier Transform Infrared ◽  
Gaseous Ammonia ◽  
Tissue Water ◽  
Ammonia Exposure

Chemical injury to the eye is still an important cause of blindness and serious complications. Gaseous ammonia combines with tissue water to form ammonium hydroxide ( NH 4 OH ). This exothermic reaction results in both heat and chemical burns. Although, over the years, the different biological effects of anhydrous ammonia are well known, its ocular effects are less clearly documented. This study reports the corneal structural alterations that may be induced as a result of ammonia exposure (gas or liquid) that was studied by Fourier transform infrared spectroscopy. The resulted IR spectra were analyzed using the band enhancement procedure. The obtained data clearly indicate that there are different structural and conformational changes (includes lipids and proteins) as the method of exposure to the ammonia differ, and due to the increased ammonia occupational exposure, there is an insistent need for the development of ophthalmic medications.

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