The effect of Q‐switched 1064‐nm Nd: YAG laser on skin barrier and collagen synthesis via miR‐663a to regulate TGFβ1/smad3/p38MAPK pathway

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

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In vivo assessment of the effect of a cream containing Avena Rhealba® extract and hyaluronic acid on the restoration of the skin barrier in de-epidermised skin produced with an erbium-YAG laser

European Journal of Dermatology ◽

10.1684/ejd.2014.2406 ◽

2014 ◽

Vol 24 (5) ◽

pp. 583-588 ◽

Cited By ~ 1

Author(s):

Mélanie Sabadotto ◽

Jennifer Theunis ◽

David Black ◽

Valérie Mengeaud ◽

Anne-Marie Schmitt

Keyword(s):

Hyaluronic Acid ◽

Skin Barrier ◽

Yag Laser ◽

In Vivo Assessment

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Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Current Developments in Nutrition ◽

10.1093/cdn/nzaa045_020 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 387-387

Author(s):

Barbara Davis ◽

Dong Ju Son ◽

Jae Chul Jung ◽

Yong Min Choi ◽

Hyeon Yeol Ryu ◽

...

Keyword(s):

Oral Administration ◽

Barrier Function ◽

Collagen Synthesis ◽

Skin Barrier ◽

Human Keratinocytes ◽

Water Evaporation ◽

Skin Barrier Function ◽

Type I ◽

Skin Damage ◽

Hairless Mice

Abstract Objectives The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. Methods The ability for repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. To complement this work, and better understand the mechanism(s) through which this dietary ingredient works, changes in procollagen, hyaluronic acid (HA) and matrix metalloproteinase-1 (MMP-1) levels were assessed in response to UVB treatment in the presence and absence of WEO. Results The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, HA, and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of MMP-1, since its induction by UVB treatment was diminished in treated CCD-986sk cells. Conclusions Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption. Funding Sources Funding for this research was provided by the Life Science Research Institute, Novarex Co., Ltd.

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The effect of Q-switched 1064-nm dymium-doped yttrium aluminum garnet laser on the skin barrier and collagen synthesis through miR-24-3p

Lasers in Medical Science ◽

10.1007/s10103-020-03214-9 ◽

2021 ◽

Author(s):

Zhi Yang ◽

Xiaoxia Duan ◽

Xue Wang ◽

Dongqing Li ◽

Qi Xu ◽

...

Keyword(s):

Western Blotting ◽

Collagen Synthesis ◽

Yttrium Aluminum Garnet ◽

Population Aging ◽

Skin Barrier ◽

Skin Aging ◽

Luciferase Reporter ◽

Protective Effects ◽

Luciferase Reporter Gene ◽

Skin Collagen

AbstractDue to the increase of the world’s population aging, how to restore youthfulness to the skin has attracted much attention. It is well known that collagen synthesis and changes in skin barrier play an important role in the process of skin aging. However, whether Q-switched 1064-nm Nd:YAG laser (1064-QSNYL) determines the involvement of miRNAs in skin collagen synthesis and skin barrier changes remains to be elucidated. Upstream miRNAs of p38 molecular pathway have been predicted by bioinformatic database and the relationship between miRNAs and p38 verified by dual-luciferase reporter gene and Western blotting. RT-qPCR analysis detected the expression of miR-24-3p and mRNA for collagen and skin barrier–related molecules, such as keratin 10 (K10), filaggrin, and Aquaporin 4 (APQ4), in mice back skin and in the keratinocyte cell line HaCaT. Western blotting and immunofluorescence (IF) have been used to detect collagen expression and to localize, as well as quantify K10, filaggrin, and APQ4, respectively. In this study, we show that p38 is the main target gene of miRNA-24-3p, and laser irradiation at 1.5 J/cm2 inhibits miR-24-3p expression. Irradiation treatment upregulates the moisture, elasticity, hydroxyproline, and superoxide dismutase content of mice skin, as well as inhibits trans-epidermal water loss. Irradiation also increases collagen, K10, filaggrin, and APQ4 in both mice skin and HaCaT cells. Interestingly, we found that miR-24-3p overexpression inhibits the effect of irradiation on collagen synthesis and skin barrier. We show for the first time that 1064-QSNYL promotes collagen synthesis and protective effects on skin barrier by downregulating miR-24-3p.

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Changes in corneocyte element concentrations occur in skin inner stratum corneum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100134326 ◽

1990 ◽

Vol 48 (2) ◽

pp. 146-147

Author(s):

R. R. Warner

Keyword(s):

Stratum Corneum ◽

Human Skin ◽

Element Concentration ◽

Skin Barrier ◽

Barrier Properties ◽

Brick Wall ◽

Inorganic Elements ◽

Dry Skin ◽

Skin Biopsies ◽

Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

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