Does soil warming affect the interaction between Pasteuria penetrans and Meloidogyne javanica in tomato plants?

2018 ◽  
Vol 67 (8) ◽  
pp. 1777-1783 ◽  
Author(s):  
E. A. Lopes ◽  
J. N. Orr ◽  
V. C. Blok
Keyword(s):  
Meloidogyne Javanica ◽  
Pasteuria Penetrans ◽  
Soil Warming ◽  
Tomato Plants

The relationships of time and temperature to body weight and numbers of endospores in Pasteuria penetrans-infected Meloidogyne javanica females

Nematology ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Daim Ali Darban ◽  
Barbara Pembroke ◽  
Simon Gowen
Keyword(s):  
Body Weight ◽  
Meloidogyne Javanica ◽  
Pasteuria Penetrans ◽  
Tomato Plants ◽  
Rate Of Increase ◽  
Growth Room

Abstract Tomato plants inoculated with Meloidogyne javanica juveniles infected with Pasteuria penetrans were grown in a glasshouse (20-32°C) for 36, 53, 71 and 88 days and in a growth room (26-29°C) for 36, 53, 71 and 80 days. Over these periods the numbers of P.penetrans endospores in infected M. javanica females and the weights of individual infected females increased. In the growth room, most spores (2.03 106) were found after 71 days. However, in the glasshouse the rate of increase was slower and spore numbers were still increasing at the final sampling at 88 days (2.04 106), as was the weight of the nematodes (72 μg). Weights of uninfected females reached a maximum of 36.2 and 43.1 μg after 71 days in the growth room and glasshouse, respectively.

Factors affecting the adhesion of micro-organisms to the surfaces of plant-parasitic nematodes

Parasitology ◽  
1989 ◽  
Vol 98 (1) ◽  
pp. 155-164 ◽  
Author(s):  
A. F. Bird ◽  
Ingrid Bonig ◽  
A. Bacic
Keyword(s):  
Meloidogyne Javanica ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Pasteuria Penetrans ◽  
Con A ◽  
Factors Affecting ◽  
Pas Staining ◽  
Pre Treatment ◽  
Micro Organisms ◽  
Dauer Larvae

SummaryThe influence of various agents on the adhesion of endospores of Pasteuria penetrans to the nematode Meloidogyne javanica was studied. Similarly, but to a lesser degree, we have also studied the adhesion of conidia of the fungus Dilophospora alopecuri and the coryneform bacterium Clavibacter sp. (syn. Corynebacterium rathayi) to the nematode Anguina agrostis (syn. A. funesta). Reduction in the degree of both spore and conidial attachment following their pre-treatment with periodate and the presence of PAS staining material on spores, conidia and bacteria implicated carbohydrate in these interactions. Tests involving both unbound and FITC-bound lectins demonstrated that wheat germ agglutinin (WGA) can inhibit the degree of attachment of P. penetrans to M. javanica and that this inhibition can be overcome by pre-treatment of the lectin with N, N′-diacetyl chitobiose. Endospores of P. penetrans, amphid and buccal secretions of 2nd-stage larvae of M. javanica and the cuticle and excretory pore secretions of 2nd-stage dauer larvae of A. agrostis bound WGA, indicating that accessible N-acetyl-D-glucosamine residues are present on these structures. Endospores of P. penetrans also bound Con A, indicating the presence of accessible α-D-glucose/α-D-mannose residues on their surface.

Cover crops and Pochonia chlamydosporia for the control of Meloidogyne javanica

Nematology ◽  
2011 ◽  
Vol 13 (8) ◽  
pp. 919-926 ◽  
Author(s):  
Guilherme S. de Podestá ◽  
Rosangela Dallemole -Giaretta ◽  
Silamar Ferraz ◽  
Ernani Luis Agnes ◽  
Leandro Grasside Freitas ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Cover Crops ◽  
Soil Cover ◽  
Meloidogyne Javanica ◽  
Infested Soil ◽  
Tomato Seedling ◽  
Tomato Plants ◽  
Pochonia Chlamydosporia ◽  
Colony Forming Units ◽  
Tillage System

AbstractThe objective of this research was to evaluate the effect of the combination of Pochonia chlamydosporia var. chlamydosporia with summer and winter cover plants on the control of Meloidogyne javanica on tomato plants under glasshouse conditions. Treatment combinations were with four soil covers (pearl millet and Surinam grass in Experiment 1, oil radish and black oat in Experiment 2; plus tomato and fallow controls) and two P. chlamydosporia treatments (with or without the fungus). The antagonist was applied to nematode-infested soil when the cover crops or tomato were planted. Tomato plants were removed and the above-ground parts of the cover crops were cut, dried, and placed on the pots 60 days after planting. One tomato seedling was transplanted in each pot in a no-tillage system and cultivated for 60 days. Surinam grass, pearl millet and black oat reduced galls and eggs of M. javanica by more than 90%, without application of the fungus. However, P. chlamydosporia + Surinam grass significantly reduced by 72% the number of galls compared with cultivation of the grass in soil without the fungus. Pochonia chlamydosporia became established in soil and could be re-isolated at the end of both experiments. Colony forming units (CFU) (g soil)–1 varied from 1.0 × 105 (fallow) to 2.6 × 105 (pearl millet) and from 1.1 × 105 (fallow) to 2.3 × 105 (oil radish) for the experiments with summer soil cover crops and winter soil cover crops, respectively. The cultivation of Surinam grass, pearl millet and black oat reduced M. javanica populations, and the combination with P. chlamydosporia may favour the establishment of the fungus in the soil and enhance the control of the nematode.

The isolation of a single spore isolate of Pasteuria penetrans and its pathogenicity on Meloidogyne javanica

Nematology ◽  
2004 ◽  
Vol 6 (4) ◽  
pp. 463-471 ◽  
Author(s):  
James R. Trotter ◽  
Daim Ali Darban ◽  
Simon R. Gowen ◽  
Alistair H. Bishop ◽  
Barbara Pembroke
Keyword(s):  
Dna Sequences ◽  
Meloidogyne Javanica ◽  
Egg Mass ◽  
Parental Population ◽  
Pasteuria Penetrans ◽  
Root Knot Nematode ◽  
Single Spore ◽  
Mass Line ◽  
Single Spore Isolate ◽  
Spore Population

Abstract We have obtained a single spore isolate of Pasteuria penetrans, derived by allowing a single spore to attach to a secondstage juvenile (J2) of the root-knot nematode Meloidogyne javanica. By analysing DNA sequences at three different loci we have obtained evidence that the isolate is, indeed, genetically pure. We compared the ability of the single spore isolate and the parent population from which it was selected to attach to and parasitise both the original population of M. javanica on which it was isolated and a single egg mass line derived from it. There was no difference in the attachment of spores of the single spore isolate to juveniles compared to the parental population, although there were higher numbers of both attaching to J2 of the single egg mass line compared to its parental population. Judging from the numbers of egg masses and Pasteuria -infected females, the single spore isolate was less pathogenic to the parental population of M. javanica than was the parental spore population.

scholarly journals Attachment of Pasteuria penetrans spores on populations of Meloidogyne javanica and M. incognita virulent and avirulent on the Mi gene of resistant tomato

2008 ◽  
Vol 45 (1) ◽  
pp. 54-56 ◽  
Author(s):  
E. Tzortzakakis
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Pasteuria Penetrans ◽  
Mi Gene ◽  
Spore Attachment

AbstractSpore attachment of three Pasteuria penetrans isolates was assessed on juveniles of Meloidogyne incognita and Meloidogyne javanica populations from Greece. The nematode populations differed in their ability to reproduce on tomatoes with the Mi gene, conferring resistance to rootknot nematodes. The numbers of attached spores did not discriminate virulent from avirulent populations within each species. The differences in attachment rates probably reflect the specificity of Pasteuria penetrans to different Meloidogyne populations of the same species, within a country or even the same area.

scholarly journals EFFECT OF THE AQUEOUS EXTRACT OF THE MINT (Mentha sp.) AND Lantana camara FOR Meloidogyne javanica IN TOMATO PLANTS IN A GREENHOUSE CONDITION

Nucleus ◽  
2016 ◽  
Vol 13 (1) ◽  
pp. 15-24
Author(s):  
Bianca Oliveira Gonçalves Costa ◽  
Misael Umberto de Oliveira ◽  
Kenji Cláudio Augusto Senô
Keyword(s):  
Aqueous Extract ◽  
Meloidogyne Javanica ◽  
Lantana Camara ◽  
Greenhouse Condition ◽  
Tomato Plants

scholarly journals CONTROLE DE MELOIDOGYNE JAVANICA COM PASTEURIA PENETRANS

1999 ◽  
Vol 34 (11) ◽  
pp. 2065-2069 ◽  
Author(s):  
RAVI DATT SHARMA ◽  
LÚCIO JOSÉ VIVALDI
Keyword(s):  
Meloidogyne Javanica ◽  
Pasteuria Penetrans

Objetivou-se, com esse trabalho, avaliar a eficiência de Pasteuria penetrans no controle de Meloidogyne javanica em condições de casa de vegetação. Os tratamentos eram compostos de quatro níveis de inóculo de P. penetrans, 0, 10x10(5), 50x10(5) e 100x10(5) endósporos/kg de solo autoclavado. Imediatamente após a inoculação da bactéria P. penetrans no solo autoclavado, 1.000 juvenis de segundo estádio de M. javanica foram inoculados em cada vaso. Quarenta e oito horas após a inoculação do nematóide, uma plântula de soja cv. FT-Cristalina, com três dias de idade, foi transplantada para cada vaso. O experimento foi avaliado em duas etapas: a primeira, 89 dias após o transplantio da soja, e a segunda, 90 dias após um segundo()plantio de soja, em seqüência a um pousio de 30 dias. Na primeira avaliação, o maior peso da matéria fresca da planta foi obtido no tratamento com 100x10(5) endósporos/kg de solo, o que diferiu significativamente (P<0,05) dos demais tratamentos, exceto o da testemunha. O maior aumento no peso da matéria fresca das vagens, obtido no tratamento com 100x10(5) endósporos/kg de solo, diferiu significativamente (P<0,05) dos demais tratamentos. A redução da população final do nematóide nos tratamentos com 10x10(5), 50x10(5) e 100x10(5) endósporos/kg de solo, em relação à testemunha, foi de 50,9%, 89,1% e 81,8%, respectivamente. Na segunda avaliação, o controle do nematóide nos níveis de 10x10(5), 50x10(5) e 100x10(5) endósporos/kg de solo foi de 94,7%, 99,7% e 100%, respectivamente. P. penetrans mostrou-se altamente eficiente no controle de M. javanica, mesmo no nível mais baixo de inóculo (10x10(5) endósporos/kg de solo).

Sign in / Sign up

Export Citation Format

Share Document