scholarly journals EFFECT OF THE AQUEOUS EXTRACT OF THE MINT (Mentha sp.) AND Lantana camara FOR Meloidogyne javanica IN TOMATO PLANTS IN A GREENHOUSE CONDITION

Nucleus ◽  
2016 ◽  
Vol 13 (1) ◽  
pp. 15-24
Author(s):  
Bianca Oliveira Gonçalves Costa ◽  
Misael Umberto de Oliveira ◽  
Kenji Cláudio Augusto Senô
Keyword(s):  
Aqueous Extract ◽  
Meloidogyne Javanica ◽  
Lantana Camara ◽  
Greenhouse Condition ◽  
Tomato Plants

scholarly journals Utilization of Cladophora glomerata extract nanoparticles as eco-nematicide and enhancing the defense responses of tomato plants infected by Meloidogyne javanica

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rehab Y. Ghareeb ◽  
Hanan Alfy ◽  
Antwan A. Fahmy ◽  
Hayssam M. Ali ◽  
Nader R. Abdelsalam
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Meloidogyne Javanica ◽  
Phenol Oxidase ◽  
Defense Responses ◽  
Cladophora Glomerata ◽  
Ag Nps ◽  
Root Knot Nematodes ◽  
Tomato Plants ◽  
Egg Hatchability

AbstractTomato (Solanum Lycopersicum L.) is an important vegetable crop that belongs to the family Solanaceae. Root-knot nematodes reflect the highly critical economically damaging genera of phytoparasitic nematodes on tomato plants. In this study, the eco-nematicide activity of freshwater green macroalga Cladophora glomerata aqueous extract and their synthesized silver nanoparticles (Ag-NPs) against root-knot nematodes Meloidogyne javanica was investigated on tomato plants. The formation and chemical structure of Ag-NPs was examined. The aqueous extract from C. glomerata was applied against the root-knot nematodes besides the biosynthesized green silver nanoparticles with 100, 75, 50, and 25% (S, S/2, S/3, S/4) concentrations. To investigate the plant response toward the Green Synthesized Silver Nanoparticles (GSNPs) treatment, expression profiling of Phenylalanine Ammonia-Lyase (PAL), Poly Phenol Oxidase (PPO), and Peroxidase (POX) in tomato were examined using Quantitative Real-Time PCR (Q-PCR). The results indicated that GSNPs from C. glomerata exhibited the highest eco-nematicide activity in the laboratory bioassay on egg hatchability and juveniles (J2S) mortality of M. javanica compared with the chemical commercial nematicide Rugby 60%. Also, results showed a significant reduction in galls number, egg masses, females per root system/plant, and mortality of juveniles. The results of PAL and PPO enzyme expression for the control plants remained relatively stable, while the plant inoculated with nematode M. javanica as well as the activity of genes in scope was increased from 14 to 28 Days after Nematode Inoculation (DANI). These activities were improved in inoculated plants and treated with C. glomerata extract and their green syntheses of Ag-NPs and the other plants treated with Rugby 60% (4 mL/L). The greatest activities of the three enzymes were evident after 14 days after the nematode inoculation. It can be concluded that the green synthesized nanoparticles using C. glomerata could be used as potent nematicides against M. javanica which induces the immune system to defend against nematode infection.

scholarly journals Bioefficacy of local Lantana camara (Verberneae) plant extracts against the 3rd instar larva and adult stages of Anopheles gambiae senso lato (Giles).

2020 ◽  
Vol 3 (12) ◽  
pp. 1120-1129
Author(s):  
Wenceslas Yana ◽  
Enda Corinna Andu ◽  
Katamssadan Haman Tofel ◽  
Abe Henri
Keyword(s):  
Anopheles Gambiae ◽  
Aqueous Extract ◽  
Exposure Time ◽  
Larval Mortality ◽  
Acetone Extract ◽  
Lantana Camara ◽  
Hexane Extract ◽  
Lethal Concentration ◽  
Malaria Vectors ◽  
Malaria Vector Control

Resistance of malaria vectors to synthetic chemicals with high operational cost and environmental pollution has been a great challenge to scientists. Alternative approaches such as the use of natural plant products which are environmentally friendly are put in place to control malaria vectors. This study was focused on testing the effectiveness of three solvent extracts of Lantana camara on the 3rd instar larvae and adults of Anopheles gambiae s. l. These extracts were obtained by maceration. Bioassays test were carried out by WHO’s method for determination of larvicidal and adulticidal efficacy. The results show that, larval mortality increased significantly with the concentration and exposure time. Lethal concentrations 50 (LC50) and 95 (LC95) after 24 hours of larvae exposure time are respectively 0.31 g/mL and 1.53 g/mL  while within 48 h they are 0.27 g/mL and 0.79 g/mL for hexane extract; 1.45 g/mL and 2.0 g/mL (24 h exposure), 0.84 g/mL and 1.55 g/mL (48 h exposure) for acetone extract; 1.96 g/mL and no lethal concentration causing 95% mortality was determined; 0.40 g/mL and 2.20 g/mL (48 h) for aqueous extract. The efficacy of hexane and aqueous extract on the adult knock down and mortality were not significant even with the increasing extract concentrations and exposure time whereas with acetone extract,  the adult LC50 after 24 h was 2.4 g/mL but with 95% mortality lethal concentration was not determined. According to the results, hexane extract showed high larvicidal efficacy of An. gambiae and acetone extract showed significant adult mortality. Those two extracts of L. camara can be used to fight against An. gambiae as alternative malaria vector control to replace conventional insecticides.

Cover crops and Pochonia chlamydosporia for the control of Meloidogyne javanica

Nematology ◽  
2011 ◽  
Vol 13 (8) ◽  
pp. 919-926 ◽  
Author(s):  
Guilherme S. de Podestá ◽  
Rosangela Dallemole -Giaretta ◽  
Silamar Ferraz ◽  
Ernani Luis Agnes ◽  
Leandro Grasside Freitas ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Cover Crops ◽  
Soil Cover ◽  
Meloidogyne Javanica ◽  
Infested Soil ◽  
Tomato Seedling ◽  
Tomato Plants ◽  
Pochonia Chlamydosporia ◽  
Colony Forming Units ◽  
Tillage System

AbstractThe objective of this research was to evaluate the effect of the combination of Pochonia chlamydosporia var. chlamydosporia with summer and winter cover plants on the control of Meloidogyne javanica on tomato plants under glasshouse conditions. Treatment combinations were with four soil covers (pearl millet and Surinam grass in Experiment 1, oil radish and black oat in Experiment 2; plus tomato and fallow controls) and two P. chlamydosporia treatments (with or without the fungus). The antagonist was applied to nematode-infested soil when the cover crops or tomato were planted. Tomato plants were removed and the above-ground parts of the cover crops were cut, dried, and placed on the pots 60 days after planting. One tomato seedling was transplanted in each pot in a no-tillage system and cultivated for 60 days. Surinam grass, pearl millet and black oat reduced galls and eggs of M. javanica by more than 90%, without application of the fungus. However, P. chlamydosporia + Surinam grass significantly reduced by 72% the number of galls compared with cultivation of the grass in soil without the fungus. Pochonia chlamydosporia became established in soil and could be re-isolated at the end of both experiments. Colony forming units (CFU) (g soil)–1 varied from 1.0 × 105 (fallow) to 2.6 × 105 (pearl millet) and from 1.1 × 105 (fallow) to 2.3 × 105 (oil radish) for the experiments with summer soil cover crops and winter soil cover crops, respectively. The cultivation of Surinam grass, pearl millet and black oat reduced M. javanica populations, and the combination with P. chlamydosporia may favour the establishment of the fungus in the soil and enhance the control of the nematode.

scholarly journals Antifungal activity of Ocimum gratissimum L., Lantana camara L. & Pteleopsis suberosa Engl. & Dies used in the treatment of vulvovaginal candidiasis in Benin

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jean Robert Klotoe ◽  
Brice Armand Fanou ◽  
Eric Agbodjento ◽  
Arnaud Houehou ◽  
Lauris Fah ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Lantana Camara ◽  
Vulvovaginal Candidiasis ◽  
Reference Drug ◽  
Clinical Strains ◽  
Ocimum Gratissimum

Abstract Background Vulvovaginal candidiasis is a widespread mycotic infection that affects a large proportion of women of childbearing age. Its management in traditional medicine is based on the use of medicinal plants. This study aimed to evaluate the antifungal activity of Ocimum gratissimum L., Lantana camara L. and Pteleopsis suberosa Engl. & Diels used in the treatment of vulvovaginal candidiasis in Benin. Results The data obtained from the in vitro antifungal test show that the strains tested (ATCC 90028 and two clinical strains: 1MA and 3MA) were more sensitive to aqueous extracts with a better effect for Pteleopsis suberosa. This potential of the tested extracts correlated with their richness in total polyphenols. The extract of the Pteleopsis suberosa was very active on the inhibition of the reference strain ATCC 90028. On the clinical strains (1MA and 3MA) the aqueous extract of Pteleopsis suberosa showed a better MIC on the 1MA strain. In vivo model, inoculation of 100 µL of the concentrated Candida albicans suspension 1.5 × 105 UFC/mL induced the candidiasis of the female Wistar rat. The treatment with the aqueous extract of Pteleopsis suberosa, like fluconazole (reference drug), significantly reduced Candida albicans infection at a dose of 100 mg/kg after 1, 7 and 13 days of treatment. Conclusion This study revealed the potential antifungal of the Ocimum gratissimum, Lantana camara and Pteleopsis suberosa. Pteleopsis suberosa has better antifungal activity in vitro and in vivo. These observations justify the use of their medicinal plant in the traditional treatment of vulvovaginal candidiasis in Benin.

scholarly journals Control of Gray Mold on Tomato plants by Spraying Piper nigrum and Urtica dioica Extracts under Greenhouse Condition

2019 ◽  
Vol 60 (5) ◽  
pp. 961-971
Author(s):  
Hamed N. Ghazal ◽  
Ayyad W Al-Shahwany ◽  
Firas T. Al–Dulaimy
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Urtica Dioica ◽  
Nano Particles ◽  
Piper Nigrum ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Greenhouse Condition ◽  
Tomato Plants ◽  
Inducing Resistance

Field experimented were examined the effects of Piper nigrum and Urtica dioica extracts on the gray mold disease in tomato that caused by Botrytis cinerea. To evaluate the inducing resistance of these extracts, many treatments were sprayed on tomato leaves, including methanolic and aquatic extracts, Silver nano particles biosynthesis (AgNPs) and water as (control). The results indicated that the resistance of tomato plants was increased when tomato plant sprayed first with Methanolic P. nigrum extracts and after 4 hours sprayed with B. cinerea. Also, spraying with methanolic and aquatic AgNPs P. nigrum extract were reduced gray mold disease. These results were showed that P. nigrum AgNPs treatment reduced the gray mold of tomato leaves because of the activities of total phenolic compounds which was infected with Botrytis cinerea.

scholarly journals Formulation of Improved Traditional Drugs against virulent species of Salmonella spp in Benin: assessment of the properties from Uvaria chamae, Lantana camara and Phyllantus amarus in Benin, West Africa

2019 ◽  
Author(s):  
Boris LEGBA ◽  
Victorien DOUGNON ◽  
Carène GBAGUIDI ◽  
Alidah ANIAMBOSSOU ◽  
Esther DEGUENON ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Bacterial Load ◽  
Scientific Data ◽  
Lantana Camara ◽  
Toxicity Tests ◽  
In Vitro Tests ◽  
Post Inoculation ◽  
Salmonella Spp

Abstract Background Uvaria chamae (Annonaceae), Phyllantus amarus (Phyllantaceae) and Lantana camara (Verbenaceae) are empirically alleged to be used as Beninese medicinal plants in the treatment of salmonellosis. This study aimed to produce scientific data on in vitro and in vivo efficacy of Uvaria chamae, Lantana camara and Phyllantus amarus on multiresistant Salmonella spp isolated in Benin.Results After in vitro tests on aqueous and ethanolic extracts of Uvaria chamae, Lantana camara and Phyllantus amarus , only the aqueous extract of Uvaria chamae (leaves) showed the best anti- Salmonella ’s activity. It has been used for the following experiments. The induction of salmonellosis revealed 9.0 10 8 CFU/ml was optimal concentration for triggering and maintaining the symptoms in chicks. This infective concentration has been used for in vivo assessment. 24 hours post inoculation later, the symptoms of salmonellosis (wet cloaca, diarrhea stool and somnolence) were observed in infected groups. After seven days of treatment, the rate of reduction of bacterial load at 100 mg / L, 200 mg / L, 400 mg / L of this extract was 85%, 52.38% and 98% respectively in the chicks groups infected with Salmonella Typhimurium ATCC 14028. About the groups infected with Salmonella spp (virulent strain), the rate of reduction of bacterial load at 100 mg / L, 200 mg / L, 400 mg / L of this extract was 0%, 98.66% and 99.33%. The toxicity tests did not show any significant effect of the Uvaria chamae ’s extract on the biochemical and hematological parameters of the chicks.Conclusion The aqueous extract of Uvaria chamae is active in vitro and in vivo on multiresistant strains of Salmonella spp . This plant is a good candidate for the development of an improved traditional medicine for the management of salmonellosis.

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