Genetic variability and molecular evolution of arabis mosaic virus based on the coat protein ( CP ) gene sequence

2021 ◽  
Author(s):  
Beata Komorowska ◽  
Beata Hasiów‐Jaroszewska ◽  
Daria Budzyńska
Keyword(s):  
Molecular Evolution ◽  
Coat Protein ◽  
Genetic Variability ◽  
Mosaic Virus ◽  
Gene Sequence ◽  
Arabis Mosaic Virus ◽  
Cp Gene

Genetic diversity and molecular evolution of arabis mosaic virus based on the CP gene sequence

2016 ◽  
Vol 161 (4) ◽  
pp. 1047-1051 ◽  
Author(s):  
Fangluan Gao ◽  
Wuzhen Lin ◽  
Jianguo Shen ◽  
Furong Liao
Keyword(s):  
Genetic Diversity ◽  
Molecular Evolution ◽  
Mosaic Virus ◽  
Gene Sequence ◽  
Arabis Mosaic Virus ◽  
Cp Gene

scholarly journals Characterization of Two Distinct Pepino Mosaic Virus Isolates from Tomato in Lithuania

2013 ◽  
Vol 19 (1) ◽  
pp. 22-27
Author(s):  
Marija Žižytė ◽  
Donatas Šneideris ◽  
Irena Zitikaitė ◽  
Laima Urbanavičienė ◽  
Juozas Staniulis
Keyword(s):  
Sequence Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Gene Sequence ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
Cp Gene ◽  
Gene Sequence Analysis ◽  
Virus Isolates

Abstract Two isolates of Pepino mosaic virus (PepMV) from tomato plants grown in different commercial greenhouses in Lithuania were characterized by coat protein (CP) gene sequence analysis. Comparison with other PepMV isolates from the GenBank database showed that both Lithuanian PepMV isolates share 78.3% nucleotide identity and belong to two distinct EU and CH2 genotypes of PepMV. This is the first report on characterization of two PepMV genotypes detected in Lithuania.

scholarly journals Identification, Characterization, and Molecular Detection of Alfalfa mosaic virus in Potato

2006 ◽  
Vol 96 (11) ◽  
pp. 1237-1242 ◽  
Author(s):  
H. Xu ◽  
J. Nie
Keyword(s):  
Mosaic Virus ◽  
Gene Sequence ◽  
Alfalfa Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Polymorphism Analysis ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Simple Method ◽  
Cp Gene ◽  
Nucleotide Sequence Alignment

Alfalfa mosaic virus (AMV) was detected in potato fields in several provinces in Canada and characterized by bioassay, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction (RT-PCR). The identity of eight Canadian potato AMV isolates was confirmed by sequence analysis of their coat protein (CP) gene. Sequence and phylogenetic analysis indicated that these eight AMV potato isolates fell into one strain group, whereas a slight difference between Ca175 and the other Canadian AMV isolates was revealed. The Canadian AMV isolates, except Ca175, clustered together among other strains based on alignment of the CP gene sequence. To detect the virus, a pair of primers, AMV-F and AMV-R, specific to the AMV CP gene, was designed based on the nucleotide sequence alignment of known AMV strains. Evaluations showed that RT-PCR using this primer set was specific and sensitive for detecting AMV in potato leaf and tuber samples. AMV RNAs were easily detected in composite samples of 400 to 800 potato leaves or 200 to 400 tubers. Restriction analysis of PCR amplicons with SacI was a simple method for the confirmation of PCR tests. Thus, RT-PCR followed by restriction fragment length polymorphism analysis may be a useful approach for screening potato samples on a large scale for the presence of AMV.

scholarly journals Genetic variability and evolutionary analyses of the coat protein gene of Tomato mosaic virus

Virus Genes ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 435-438 ◽  
Author(s):  
E. A. Rangel ◽  
A. Alfaro-Fernández ◽  
M. I. Font-San-Ambrosio ◽  
M. Luis-Arteaga ◽  
L. Rubio
Keyword(s):  
Coat Protein ◽  
Genetic Variability ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Tomato Mosaic Virus

scholarly journals A Begomovirus Associated with Leaf Curling and Chlorosis of Soybean in Sinaloa, Mexico is Related to Pepper golden mosaic virus

Plant Disease ◽  
2006 ◽  
Vol 90 (1) ◽  
pp. 109-109 ◽  
Author(s):  
J. Méndez-Lozano ◽  
E. Quintero-Zamora ◽  
M. P. Barbosa-Jasso ◽  
N. E. Leyva-López ◽  
J. A. Garzón-Tiznado ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Intergenic Region ◽  
Pcr Amplification ◽  
Nucleotide Identity ◽  
Homologous Region ◽  
Degenerate Primers ◽  
Sequence Comparisons ◽  
Leaf Curling ◽  
Cp Gene

Since June 2001, symptoms of yellowing, leaf curling, crumpling, and stunted growth were observed on soybean (Glycine max Merr.) plants in Sinaloa, Mexico. These symptoms and the presence of whiteflies (Bemisia tabaci Gennadius) in the affected fields suggested a viral etiology. Samples from symptomatic plants were collected from commercial fields and analyzed for the presence of begomoviruses using DNA hybridization, and as a probe, the DNA A of Pepper huasteco virus at low stringency (2). Thirty-five positive samples were subsequently used for polymerase chain reaction (PCR) amplification with the degenerate primers RepMot and CPMot (1). These primers direct the amplification of a DNA A segment comprising the entire intergenic region (IR) and the first 210 bp of the coat protein (CP) gene, which is highly variable in size and nucleotide sequence among begomoviruses. PCR products were obtained for 25 of 35 samples and five of these were cloned into the pGEM-T easy vector (Promega, Madison, WI) and sequenced. The 571-bp DNA sequence (GenBank Accession No. AY905553) was compared with sequences of other begomoviruses in GenBank using the Clustal alignment method (MegAlign, DNASTAR software, London). The sequence was 74 and 70% identical to the Pepper golden mosaic virus (PepGMV; GenBank Accession No. U57457) and Cabbage leaf curl virus (CaLCuV; GenBank Accession No. U65529) sequences, respectively. Interestingly, the partial coat protein gene sequence (210 nt) of this soybean-infecting virus was 98% identical to the CP gene of Tobacco apical stunt virus (TbASV; GenBank Accession No. AF076855). Nonetheless, the known sequence of TbASV intergenic region (GenBank Accession No. AF077744) is very different from the homologous region of the soybean virus (34% of nucleotide identity). Analysis of the soybean virus intergenic region revealed that it harbors almost identical iterons (i.e., Rep-binding sites) to PepGMV, suggesting a close relationship between these two viruses. Soybean-infecting geminiviruses have been previously reported only from Asia; however, the partial sequence of a begomovirus isolated from soybean in Brazil was recently deposited in Genbank (Accession No. AY436328). Sequence comparisons between the Brazilian and Mexican isolates showed these viruses are less related with a nucleotide identity of 46%. Taken together, our data indicate that the virus identified in this study might be either a different strain of PepGMV adapted to leguminous plants or a new begomovirus species. To our knowledge, this is the first report of a begomovirus infecting soybean in Mexico. References: (1) J. T. Ascencio-Ibañez et al. Plant Dis. 86:692, 2002. (2) J. Méndez-Lozano et al. Phytopathology 93:270, 2003.

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