scholarly journals Characterization of Two Distinct Pepino Mosaic Virus Isolates from Tomato in Lithuania

2013 ◽  
Vol 19 (1) ◽  
pp. 22-27
Author(s):  
Marija Žižytė ◽  
Donatas Šneideris ◽  
Irena Zitikaitė ◽  
Laima Urbanavičienė ◽  
Juozas Staniulis
Keyword(s):  
Sequence Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Gene Sequence ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
Cp Gene ◽  
Gene Sequence Analysis ◽  
Virus Isolates

Abstract Two isolates of Pepino mosaic virus (PepMV) from tomato plants grown in different commercial greenhouses in Lithuania were characterized by coat protein (CP) gene sequence analysis. Comparison with other PepMV isolates from the GenBank database showed that both Lithuanian PepMV isolates share 78.3% nucleotide identity and belong to two distinct EU and CH2 genotypes of PepMV. This is the first report on characterization of two PepMV genotypes detected in Lithuania.

Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination

2007 ◽  
Vol 121 (2) ◽  
pp. 131-146 ◽  
Author(s):  
Inge M. Hanssen ◽  
Anneleen Paeleman ◽  
Lieve Wittemans ◽  
Kris Goen ◽  
Bart Lievens ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genetic Characterization ◽  
Genetic Recombination ◽  
Pepino Mosaic Virus ◽  
Greenhouse Tomatoes ◽  
Virus Isolates

Purification and characterization of ?-xylosidase from Streptomyces sp. CH7 and its gene sequence analysis

2004 ◽  
Vol 20 (7) ◽  
pp. 727-733 ◽  
Author(s):  
P. Pinphanichakarn ◽  
T. Tangsakul ◽  
T. Thongnumwon ◽  
Y. Talawanich ◽  
A. Thamchaipenet
Keyword(s):  
Sequence Analysis ◽  
Gene Sequence ◽  
Purification And Characterization ◽  
Streptomyces Sp ◽  
Gene Sequence Analysis ◽  
Its Gene

scholarly journals Characterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran

2013 ◽  
Vol 11 (2) ◽  
pp. x-x ◽  
Author(s):  
Nazanin Arafati ◽  
Shirin Farzadfar ◽  
Reza Pourrahim
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Isolates

Identification and Molecular Characterization of Mung Bean Yellow Mosaic Virus in French Bean through Coat Protein Gene

2020 ◽  
Author(s):  
T.C. Archith ◽  
V. Devappa ◽  
B. Manjunath ◽  
Chirag Reddy
Keyword(s):  
Coat Protein ◽  
Early Detection ◽  
Mosaic Virus ◽  
Molecular Characterization ◽  
Mung Bean ◽  
French Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Cp Gene

Background: French bean (Phaseolus vulgaris L.), is one of the important legume vegetables grown in India. The disease mung bean yellow mosaic virus (MYMV) limits successful production of beans. MYMV has become an epidemic in bean growing regions, particularly in the tropical and subtropical countries. In summer, the disease is more serious and widely distributed and reported in various countries. The current study is aimed at identification of MYMV and molecular characterization of mung bean yellow mosaic virus in French bean. This study will be helpful for early detection of the virus, so that better preventive measures can be taken to control MYMV.Methods: The investigations were conducted during 2016-2017, all the agro climatic zones of Karnataka province were surveyed. The coat protein (CP) gene of MYMV was amplified using gene specific primer with DNA isolated from MYMV infected leaves samples in french bean. Polymerase chain reaction products were Sequenced and analysed using a bioinformatic tool. Result: The CP gene decrypt sequences analysis revealed that the identity and similarity in global alignment for all the obtained sequences ranging from 80.8 to 95.3% with reference to MYMV, whereas for horse gram yellow mosaic virus (HYMV) it ranges from 90.4 to 99.1%. Two distinct yellow mosaic viruses infecting french bean (MYMV and HYMV) were identified and it was observed that there exists considerable genetic variation among these species. Present work showed that the CP region is efficient enough to provide a simple, rapid and reliable method for early detection of MYMV infections in french bean, which would help to develop proper management strategies to control these viruses.

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