EXPRESSION OF LACTOFERRIN IN PICHIA PASTORIS INDUCTION BY GLUCOSE IN A MODIFIED G1 PROMOTER SYSTEM AND ITS ANTIMICROBIAL AND ANTITUMOR ACTIVITIES

Respirology ◽  
2017 ◽  
Vol 22 ◽  
pp. 129-130
Keyword(s):  
Pichia Pastoris ◽  
Antitumor Activities ◽  
Promoter System

scholarly journals A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Vignesh Rajamanickam ◽  
Karl Metzger ◽  
Christian Schmid ◽  
Oliver Spadiut
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Promoter System

Enhancing the expression of recombinant small laccase in Pichia pastoris by a double promoter system and application in antibiotics degradation

2021 ◽  
Author(s):  
Deepti Yadav ◽  
Bibhuti Ranjan ◽  
Nokuthula Mchunu ◽  
Marilize Le Roes-Hill ◽  
Tukayi Kudanga
Keyword(s):  
Pichia Pastoris ◽  
Promoter System ◽  
Double Promoter

Antitumor activities of some Bellis perennis L. fractions

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
F Pehlivan Karakas ◽  
A Ucar Turker ◽  
F Yalçin ◽  
I Çalis
Keyword(s):  
Antitumor Activities

Evaluation of Antioxidant and Antitumor Activities of Some Prepared Nitrogen Heterocycles

2011 ◽  
Vol 3 (7) ◽  
pp. 106-110
Author(s):  
Mohamed Abd El-Moneim ◽  
◽  
Ibrahim M El-Deen ◽  
Wessam Abd El-Fattah
Keyword(s):  
Nitrogen Heterocycles ◽  
Antitumor Activities

Optimization of fermentation conditions for the production of recombinant hpdgf-bb in Pichia pastoris

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Duong Long Duy ◽  
Pham Minh Vu ◽  
Nguyen Tri Nhan ◽  
Tran Linh Thuoc ◽  
Dang Thi Phuong Thao
Keyword(s):  
Pichia Pastoris ◽  
Fermentation Conditions ◽  
Optimization Of Fermentation

Synthesis of Some New Tetracyclic Pyrimidine Derivatives Using Exocyclic α,β-Unsaturated Ketone and Evaluation of Their Antitumor Activities

2016 ◽  
Vol 12 (8) ◽  
pp. 311-317
Author(s):  
Kamelia El-Mahdy
Keyword(s):  
Antitumor Activity ◽  
Spectroscopic Data ◽  
Acid Hydrazide ◽  
Chloroacetic Acid ◽  
Equimolar Amount ◽  
Unsaturated Ketone ◽  
Cyanoacetic Acid ◽  
Antitumor Activities ◽  
Pyrimidine Derivatives ◽  
Cyanoacetic Acid Hydrazide

Thiazolopyrimidine 2 was obtained from the reaction of dihydropyrimidinone with chloroacetic acid and benzaldehyde. Thiazolopyrimidine 2 containing an α,β-unsaturated ketonic function [-CH=CH-CO-] has been used as a component of Michael addition with an equimolar amount of dinucleophiles to give a series of novel tetracyclic pyrimidine derivatives. Treatment of thiazolopyrimidine 2 with uracil, aminotriazole, cyanoacetic acid hydrazide, o-phenylenediamine or diaminopyridine afforded the corresponding pyridopyrimidine, triazolopyrimidine, pyrazolone, benzodiazepine and triazepine derivative, respectively. The detailed synthesis, spectroscopic data, and antitumor activity for synthesized compounds were reported.

Cloning and Expression of Recombinant Human Insulin Gene in Pichia pastoris

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Pichia Pastoris ◽  
Human Insulin ◽  
Expression Vector ◽  
Expression System ◽  
Insulin Gene ◽  
Cloning And Expression ◽  
Pcr Analysis ◽  
Major Band ◽  
Human Insulin Gene ◽  
Pichia Pastoris Expression System

The main goal of the current study was cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand, by using two sets of specific primers contain EcoR1 and Notl restriction sites. The amplified preproinsulin gene fragment was double digested with EcoRI and Not 1 restriction enzymes, then inserted into pPIC9K expression vector. The new pPIC9K-hpi constructive expression vector was transformed by the heat-shock method into the E.coli DH5α competent cells. pPic9k –hpi, which was propagated in the positive transformant E. coli cells, was isolated from cells and then linearised by restriction enzyme SalI, then transformed into Pichia pastoris GS115 using electroporation method. Genomic DNA of His+ transformants cell was extracted and used as a template for PCR analysis. The results showed, that the pPic9k – hpi was successfully integrated into the P. pastoris genome, for selected His+ transformants clones on the anticipated band at 330 bp, which is corresponded to the theoretical molecular size of the human insulin gene. To follow the insulin expression in transformans, Tricine–SDS gel electrophoresis and Western blot analysis were conducted. The results showed a successful expression of recombinant protein was detected by the presence of a single major band with about (5.8 KDa) on the gel. These bands correspond well with the size of human insulin with the theoretical molecular weight (5.8 KDa).

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