A static and dynamic load test is carried out on the constructed Hanjiang Super-major Railway Bridge in Laohekou to ensure the reliability of the bridge and test the carrying capacity and working condition of the bridge structure. The static load test is divided into three kinds of loading conditions, each of which tests the stress and deflection of the key sections of the bridge. The transverse vibration displacement of the piers and mid-span section is measured in the dynamic load test when the train is crossing the bridge at different speeds, and the vertical dynamic deflection of the mid-span section is also tested. The natural vibration frequency, the vibration types and the damping ratio of the whole bridge are measured in the pulsating experiment. Through the comparative analysis of the experimental results and the theoretical calculation results and standard, it can be concluded that the strength, the vertical and lateral stiffness of the bridge meet the requirements of both the design and specifications, and that the bridge is in a good working condition. The test and the analysis of the experimental results of the bridge can serve as a reference to the bridges of the same type.