Development of a novel reverse transcription droplet digital PCR assay for the sensitive detection of Senecavirus A

2018 ◽  
Vol 66 (1) ◽  
pp. 517-525 ◽  
Author(s):  
Zhou Zhang ◽  
Yongning Zhang ◽  
Xiangmei Lin ◽  
Zhenhai Chen ◽  
Shaoqiang Wu
Keyword(s):  
Reverse Transcription ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Pcr Assay

Development of a sensitive and reliable reverse transcription droplet digital PCR assay for the detection of citrus yellow vein clearing virus

2018 ◽  
Vol 164 (3) ◽  
pp. 691-697 ◽  
Author(s):  
Yingjie Liu ◽  
Yingli Wang ◽  
Qin Wang ◽  
Yanhui Zhang ◽  
Wanxia Shen ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Yellow Vein ◽  
Pcr Assay ◽  
Vein Clearing

Abstract 411: Rapid and sensitive detection of EGFR C797S mutations using a blood-based droplet digital PCR assay

2019 ◽  
Author(s):  
Victoria Edwards ◽  
Leisa Jackson ◽  
Jordan Reese ◽  
Hestia S. Mellert ◽  
Gary Pestano
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Pcr Assay

Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3

2019 ◽  
Vol 43 ◽  
pp. 50-57 ◽  
Author(s):  
Yongning Zhang ◽  
Zhou Zhang ◽  
Zhanying Wang ◽  
Zili Wang ◽  
Caixia Wang ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Porcine Circovirus ◽  
Pcr Assay ◽  
Porcine Circovirus 3

Development of a novel droplet digital PCR assay for the sensitive detection of carp edema virus

Aquaculture ◽  
2021 ◽  
pp. 737162
Author(s):  
Na Wang ◽  
Min Zhang ◽  
Zhou Zhang ◽  
Hongli Jing ◽  
Shaoqiang Wu ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Pcr Assay

Abstract 411: Rapid and sensitive detection of EGFR C797S mutations using a blood-based droplet digital PCR assay

2019 ◽  
Author(s):  
Victoria Edwards ◽  
Leisa Jackson ◽  
Jordan Reese ◽  
Hestia S. Mellert ◽  
Gary Pestano
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Pcr Assay

scholarly journals Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Samuel Long ◽  
Brian Berkemeier
Keyword(s):  
Simian Immunodeficiency Virus ◽  
Reverse Transcription ◽  
Rhesus Macaques ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Sensitive Detection ◽  
Similar Reaction ◽  
Tissue Samples ◽  
Low Level ◽  
Immunodeficiency Virus

Abstract Background Simian immunodeficiency virus (SIV)-infected rhesus macaques constitute an excellent model of human HIV infection. Sensitive detection of SIV RNA in cell and tissue samples from infected animals subjected to treatment regimens becomes especially critical in determining which therapeutic attempts are successful, and consequently, which interventions should be prioritized in HIV cure research. Results In this report, we describe the design and testing of a Raindance ddPCR platform-based, sensitive SIV reverse transcription droplet digital PCR (RT-ddPCR) assay by exploring the combinations of various priming conditions and reverse transcriptases, and testing one-step vs. two-step procedures, to eliminate background signal(s) and enable detection and quantification of low level target signals. Conclusions Similar reaction conditions and assay validation procedures can be explored for potential development of additional assays for other applications that require sensitive detection of low-level targets in RNA samples.

scholarly journals Droplet‐digital PCR assay to detect Merkel cell polyomavirus sequences in chorionic villi from spontaneous abortion affected females

2019 ◽  
Vol 235 (3) ◽  
pp. 1888-1894 ◽  
Author(s):  
Andrea Tagliapietra ◽  
John Charles Rotondo ◽  
Ilaria Bononi ◽  
Elisa Mazzoni ◽  
Federica Magagnoli ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Pcr Assay ◽  
Chorionic Villi
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