scholarly journals BdWRKY38 is required for the incompatible interaction of Brachypodium distachyon with the necrotrophic fungus Rhizoctonia solani

2020 ◽  
Vol 104 (4) ◽  
pp. 995-1008
Author(s):  
Yusuke Kouzai ◽  
Minami Shimizu ◽  
Komaki Inoue ◽  
Yukiko Uehara‐Yamaguchi ◽  
Kotaro Takahagi ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Brachypodium Distachyon ◽  
Incompatible Interaction

scholarly journals Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sobhy S. H. Abdelsalam ◽  
Yusuke Kouzai ◽  
Megumi Watanabe ◽  
Komaki Inoue ◽  
Hidenori Matsui ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Agar Medium ◽  
Gene Annotation ◽  
Brachypodium Distachyon ◽  
Infection Process ◽  
Effector Proteins ◽  
Polymerase Chain ◽  
Necrotrophic Stage ◽  
Induced Immunity ◽  
Single Time Point

Abstract Rhizoctonia solani is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in rice production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block R. solani AG-1 IA infection in both rice and Brachypodium distachyon. R. solani may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. To identify pathogen genes expressed at the early infection process, here we developed an inoculation method using B. distachyon which enables to sample an increased amount of semi-synchronous infection hyphae. Sixty-one R. solani secretory effector-like protein genes (RsSEPGs) were identified using in silico approach with the publicly available gene annotation of R. solani AG-1 IA genome and our RNA-sequencing results obtained from hyphae grown on agar medium. Expression of RsSEPGs was analyzed at 6, 10, 16, 24, and 32 h after inoculation by a quantitative reverse transcription-polymerase chain reaction and 52 genes could be detected at least on a single time point tested. Their expressions showed phase-specific patterns which were classified into 6 clusters. The 23 RsSEPGs in the cluster 1–3 and 29 RsSEPGs in the cluster 4–6 are expected to be involved in biotrophic and necrotrophic interactions, respectively.

scholarly journals Salicylic acid-dependent immunity contributes to resistance against Rhizoctonia solani , a necrotrophic fungal agent of sheath blight, in rice and Brachypodium distachyon

2017 ◽  
Vol 217 (2) ◽  
pp. 771-783 ◽  
Author(s):  
Yusuke Kouzai ◽  
Mamiko Kimura ◽  
Megumi Watanabe ◽  
Kazuki Kusunoki ◽  
Daiki Osaka ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Rhizoctonia Solani ◽  
Brachypodium Distachyon ◽  
Sheath Blight

Brachypodium distachyon genotypes vary in resistance to Rhizoctonia solani AG8

2016 ◽  
Vol 43 (2) ◽  
pp. 189 ◽  
Author(s):  
Katharina Schneebeli ◽  
Ulrike Mathesius ◽  
Alexander B. Zwart ◽  
Jennifer N. Bragg ◽  
John P. Vogel ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Root Rot ◽  
Quantitative Resistance ◽  
Brachypodium Distachyon ◽  
Genetic Resistance ◽  
Sole Source ◽  
Wheat Root ◽  
Anastomosis Group ◽  
Seedling Vigour ◽  
Rhizoctonia Root Rot

Brachypodium distachyon (L.)P.Beauv. (Bd) has previously been developed as a pathosystem model for the wheat root rot pathogen Rhizoctonia solani Kühn anastomosis group 8 (AG8). Here we explore variation in resistance to R. solani AG8 in Bd, to determine whether genomic tools could be used to find Bd genes involved in the grass defence response, with the aim of using this information for the improvement of Rhizoctonia root rot resistance in wheat. We looked for variation in resistance to R. solani AG8 in a diverse Bd natural accession collection and in Bd T-DNA insertion lines selected based on putative mechanisms reported for tagged genes. All lines were susceptible to the pathogen. Repeatable and significant variation in resistance was measured in both groups, with greater variation in resistance found across the natural accessions than in the T-DNA lines. The widest and most repeatable variation in resistance was between lines Koz-3 and BdTR 13a. The ratio of R. solani AG8-inoculated to uninoculated root length for line Koz-3 was 33% greater than the same ratio for line BdTR 13a. The increased resistance of Koz-3 was associated with nodal root initiation in response to the pathogen. A negative correlation between seedling vigour and resistance was observed, but found not to be the sole source of variation in resistance to R. solani AG8. The only T-DNA line with significantly greater resistance to R. solani AG8 than the reference line had an insertion in a putative galactosyltransferase gene; however, this result needs further confirmation. Genetic resistance to Rhizoctonia root rot is not available in wheat cultivars and only a few instances of quantitative resistance to the pathogen have been described within close relatives of wheat. Brachypodium distachyon offers potential for further investigation to find genes associated with quantitative resistance and mechanisms of tolerance to R. solani AG8.

Mesure de la colonisation saprophytique en compétition de Rhizoctonia solani Kühn dans les sols et substrats

Agronomie ◽  
1981 ◽  
Vol 1 (6) ◽  
pp. 513-517 ◽  
Author(s):  
Pierre CAMPOROTA ◽  
Reine CAMPOROTA
Keyword(s):  
Rhizoctonia Solani

Sclerotia of the plant pathogen Rhizoctonia solani Kühn as a new source of bioactive compounds

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
KA Aliferis ◽  
S Jabaji
Keyword(s):  
Rhizoctonia Solani ◽  
Bioactive Compounds ◽  
Plant Pathogen

Molecular characterization of novel isolates of Rhizoctonia solani, Trichoderma atroviride and Fusarium spp. isolated from different plants and cutting woods in Iraq

2019 ◽  
Vol 52 (3) ◽  
Author(s):  
Aqeel N. Al-Abedy ◽  
R.G. Al-Janabi ◽  
Z.A. Al-Tmeme ◽  
Alaa T. Salim ◽  
Muhammad Ashfaq
Keyword(s):  
Rhizoctonia Solani ◽  
Molecular Characterization ◽  
Trichoderma Atroviride ◽  
Fusarium Spp

Detection of Rhizoctonia solani AG-2-2 IV, the Causal Agent of Large Patch of Zoysiagrass, Using Plasmid DNA as a Probe.

1998 ◽  
Vol 64 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Susumu TAKAMATSU ◽  
Manami NAKANO ◽  
Hideyuki YOKOTA ◽  
Hitoshi KUNOH
Keyword(s):  
Rhizoctonia Solani ◽  
Plasmid Dna ◽  
Causal Agent ◽  
Large Patch
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