A restorer‐of‐fertility‐like pentatricopeptide repeat protein promotes cytoplasmic male sterility in Arabidopsis thaliana

2020 ◽  
Author(s):  
Stéphanie Durand ◽  
Anthony Ricou ◽  
Matthieu Simon ◽  
Noémie Dehaene ◽  
Françoise Budar ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Pentatricopeptide Repeat ◽  
Repeat Protein ◽  
Pentatricopeptide Repeat Protein ◽  
Restorer Of Fertility

scholarly journals A pentatricopeptide repeat protein restores nap cytoplasmic male sterility in Brassica napus

2017 ◽  
Vol 68 (15) ◽  
pp. 4115-4123 ◽  
Author(s):  
Zhi Liu ◽  
Faming Dong ◽  
Xiang Wang ◽  
Tao Wang ◽  
Rui Su ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Pentatricopeptide Repeat ◽  
Repeat Protein ◽  
Pentatricopeptide Repeat Protein

scholarly journals MicroRNA400-Guided Cleavage of Pentatricopeptide Repeat Protein mRNAs Renders Arabidopsis thaliana More Susceptible to Pathogenic Bacteria and Fungi

2014 ◽  
Vol 55 (9) ◽  
pp. 1660-1668 ◽  
Author(s):  
Young Ju Park ◽  
Hwa Jung Lee ◽  
Kyung Jin Kwak ◽  
Kwanuk Lee ◽  
Suk Whan Hong ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pathogenic Bacteria ◽  
Pentatricopeptide Repeat ◽  
Repeat Protein ◽  
Pentatricopeptide Repeat Protein ◽  
Bacteria And Fungi

scholarly journals Localization of the Rf3 restorer-of-fertility gene for maize S-type cytoplasmic male sterility

2012 ◽  
Author(s):  
◽  
Tiffany Langewisch
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Candidate Gene ◽  
Male Sterility ◽  
Pollen Grains ◽  
Candidate Gene Approach ◽  
Chromosome 2 ◽  
Pentatricopeptide Repeat ◽  
Restorer Of Fertility ◽  
Ppr Proteins ◽  
Pentatricopeptide Repeat Proteins

Maize S-type cytoplasmic male sterility (CMS-S) is a maternally inherited trait that prevents pollen grains from developing to maturity. CMS-S is associated with the high levels of a novel mitochondrial transcript, orf355/orf77. Cleavage of this RNA, mediated by the nuclear restorer Rf3, reverses the sterility. Rf3 was previously mapped on the long arm of chromosome 2. The goals of this research were to fine-map the locus and to identify Rf3 using a candidate gene approach. Genotyping of nearisogenic lines (NILs) mapped Rf3 to a 1.98 Mb region of 2L. Six candidate genes, all predicted to code for mitochondrially targeted pentatricopeptide repeat proteins (PPR), were PCR-amplified, sequenced, and compared from multiple Rf3-containing NILs and non-restoring rf3 inbreds. One PPR-Rf3 candidate gene had two consistent differences between multiple restoring and non-restoring lines. Gene expression in pre-emergent tassels from the fertility-restored and non-restored plants was compared. Within the 3 Mb region surrounding Rf3, 9 genes were differentially expressed between restoring and non-restoring lines, including genes that could code for an ATP-binding protein, an ATPase, and four PPR proteins. Although Rf3 has not yet been identified, this study has revealed five promising candidates.

scholarly journals Genome-wide identification of the restorer-of-fertility-like (RFL) gene family in Brassica napus and expression analysis in Shaan2A cytoplasmic male sterility

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Luyun Ning ◽  
Hao Wang ◽  
Dianrong Li ◽  
Yonghong Li ◽  
Kang Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Gene Family ◽  
Male Sterility ◽  
Expression Analysis ◽  
Gene Clusters ◽  
Hybrid Breeding ◽  
Pentatricopeptide Repeat ◽  
Specific Expression ◽  
Restorer Of Fertility

Abstract Background Cytoplasmic male sterility (CMS) is very important in hybrid breeding. The restorer-of-fertility (Rf) nuclear genes rescue the sterile phenotype. Most of the Rf genes encode pentatricopeptide repeat (PPR) proteins. Results We investigated the restorer-of-fertility-like (RFL) gene family in Brassica napus. A total of 53 BnRFL genes were identified. While most of the BnRFL genes were distributed on 10 of the 19 chromosomes, gene clusters were identified on chromosomes A9 and C8. The number of PPR motifs in the BnRFL proteins varied from 2 to 19, and the majority of BnRFL proteins harbored more than 10 PPR motifs. An interaction network analysis was performed to predict the interacting partners of RFL proteins. Tissue-specific expression and RNA-seq analyses between the restorer line KC01 and the sterile line Shaan2A indicated that BnRFL1, BnRFL5, BnRFL6, BnRFL8, BnRFL11, BnRFL13 and BnRFL42 located in gene clusters on chromosomes A9 and C8 were highly expressed in KC01. Conclusions In the present study, identification and gene expression analysis of RFL gene family in the CMS system were conducted, and seven BnRFL genes were identified as candidates for the restorer genes in Shaan2A CMS. Taken together, this method might provide new insight into the study of Rf genes in other CMS systems.

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