First detection of human pathogenic variant of Anaplasma phagocytophilum in field‐collected Haemaphysalis longicornis , Pennsylvania, USA

2021 ◽  
Author(s):  
Keith J. Price ◽  
Bryan N. Ayres ◽  
Sarah E. Maes ◽  
Bryn J. Witmier ◽  
Holly A. Chapman ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Pathogenic Variant ◽  
Haemaphysalis Longicornis

scholarly journals Molecular Detection and Characterization of p44/msp2 Multigene Family of Anaplasma phagocytophilum from Haemaphysalis longicornis in Mie Prefecture, Japan

2019 ◽  
Vol 72 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Hongru Su ◽  
Ayaka Sato ◽  
Eri Onoda ◽  
Hiromi Fujita ◽  
Shigetoshi Sakabe ◽  
...  
Keyword(s):  
Multigene Family ◽  
Anaplasma Phagocytophilum ◽  
Molecular Detection ◽  
Haemaphysalis Longicornis

scholarly journals Incompetence of the Asian Longhorned Tick (Acari: Ixodidae) in Transmitting the Agent of Human Granulocytic Anaplasmosis in the United States

2021 ◽  
Author(s):  
Michael L Levin ◽  
Hannah M Stanley ◽  
Kris Hartzer ◽  
Alyssa N Snellgrove
Keyword(s):  
United States ◽  
Anaplasma Phagocytophilum ◽  
Vector Competence ◽  
The United States ◽  
Haemaphysalis Longicornis ◽  
Human Pathogens ◽  
Human Granulocytic Anaplasmosis ◽  
Granulocytic Anaplasmosis ◽  
Vertebrate Hosts ◽  
Transstadial Transmission

Abstract The Asian longhorned tick, Haemaphysalis longicornis Neumann (Acari: Ixodidae), was recently introduced into the United States and is now established in at least 15 states. Considering its ability for parthenogenetic propagation and propensity for creating high-density populations, there is concern that this tick may become involved in transmission cycles of endemic tick-borne human pathogens. Human granulocytic anaplasmosis (HGA) caused by Anaplasma phagocytophilum is one of the more common tick-borne diseases in the United States, especially in the northeastern and midwestern states. There is considerable geographical overlap between HGA cases and the currently known distribution of H. longicornis, which creates a potential for this tick to encounter A. phagocytophilum while feeding on naturally infected vertebrate hosts. Therefore, we evaluated the ability of H. longicornis to acquire and transmit the agent of HGA under laboratory conditions and compared it to the vector competence of I. scapularis. Haemaphysalis longicornis nymphs acquired the pathogen with the bloodmeal while feeding on infected domestic goats, but transstadial transmission was inefficient and PCR-positive adult ticks were unable to transmit the pathogen to naïve goats. Results of this study indicate that the Asian longhorned tick is not likely to play a significant role in the epidemiology of HGA in the United States.

ABOUT THE NORTHERN BOUNDARY OF DISTRIBUTION AND THE HOST SPECIES OF THE TICKS HAEMAPHYSALIS LONGICORNIS (ACARI: IXODIDAE) IN PRIMORSKY KRAI

2019 ◽  
pp. 177-182 ◽  
Author(s):  
Yu.A. Belov ◽  
◽  
T.V. Moskvina ◽  
E.M. Shchelkanov ◽  
E.G. Burukhina ◽  
...  
Keyword(s):  
Host Species ◽  
Haemaphysalis Longicornis ◽  
Primorsky Krai ◽  
Northern Boundary

An audit and insights from clinical exome sequencing in psychiatry: genotype-phenotype correlation

2020 ◽  
Author(s):  
Jayant Mahadevan ◽  
Reeteka Sud ◽  
Ravi Kumar Nadella ◽  
Vani P ◽  
Anand G Subramaniam ◽  
...  
Keyword(s):  
Exome Sequencing ◽  
Psychiatric Symptoms ◽  
Pathogenic Variant ◽  
Clinical Exome Sequencing ◽  
Mapt Gene ◽  
Nf1 Gene ◽  
Novel Variant ◽  
History Of ◽  
Atypical Presentations ◽  
Minor Physical Anomalies

BACKGROUND:Psychiatric syndromes have polymorphic symptomatology, and are known to be heritable. Psychiatric symptoms (and even syndromes) often occur as part of the clinical presentation in rare Mendelian syndromes. Clinical exome sequencing reports may help with refining diagnosis and influence treatment decisions, in addition to providing a window into the biology of brain and behaviour. We describe a clinical audit of 12 individuals who sought treatment at our hospital, and for whom targeted sequencing was ordered. Three cases are discussed in detail to demonstrate correlations between genotype and phenotype in the clinic.METHODS:Targeted Next-Generation Sequencing (NGS) was done using Clinical Exome Panel (TruSight One, Illumina) covering coding exons and flanking intronic sequences of 4811 genes associated with known inherited diseases. Variants detected were classified according to the American College for Medical Genetics (ACMG) recommendation for standards of interpretation and reporting of sequence variations.RESULTS:Ten out of twelve cases had at least one pathogenic variant. In one of these cases, we detected a known pathogenic variant in MAPT gene in a suspected FTD case, which helped us to confirm the diagnosis. In another case, we detected a novel variant predicted to be deleterious in NF1 gene. Identification of this mutation suggested a change in treatment for the patient, that was of benefit. The same patient also harboured a novel variant in the TRIO gene. This gene may be involved in biological processes that underlie the patient’s psychiatric illness.CONCLUSIONS:The cases discussed here exemplify different scenarios under which targeted exome sequencing can find meaningful application in the clinic: confirming diagnosis (MAPT variant), or modifying treatment (NF1). We suggest that clinical exome sequencing can be a helpful addition to a clinician’s toolkit when there are expediting factors to consider— such as early-onset, strong family history of mental illness, complex/atypical presentations and minor physical anomalies or neurocutaneous markers.

Sign in / Sign up

Export Citation Format

Share Document