A study of terrestrial tardigrades of the genus Milnesium Doyère, 1840 collected in Russky Island (Primorsky kray, Vladivostok) was carried out using the methods of integrative taxonomy, including the analysis of morphological and molecular biological data. Three species are recorded from this island, of which M. inceptum Morek, Suzuki, Schill, Georgiev, Yankova, Marley et Michalczyk, 2019 is new for the fauna of Russia. New data were obtained on the distribution and genetic diversity of M. tardigradum Doyère, 1840. The third species, Milnesium sp., is similar to M. tardigradum but differs from latter in the presence of a characteristic thickened cuticle zone at the base of the claws of the fourth pair of legs and is probably a new for science species.