A New Viewpoint on Genetic Diversity in Prestice Black-Pied Pig: Did the Breed Suffer from a Bottleneck?

The research was aimed at determination of genetic variability of Prestice Black-Pied (PC) pig breed (Czech national breed and genetic resource) and to evaluate possible presence of recent bottleneck in this closed small pig population. One hundred and eighty of breeding boars were analysed by eleven tetramer Short Tandem Repeats (STR) panel specifically developed for the genotyping of breeding livestock. Despite the fact that appearance of rare alleles, which may be relatively increased after recent bottleneck, was discovered, the heterozygosity excess was not significant. The PC breed has not undergone recent bottleneck and remained at mutation-drift equilibrium.

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Genetic diversity of 15 autosomal short tandem repeats loci using the AmpFLSTR®Identifiler™ kit in a Bhil Tribe Population from Gujarat state, India

Indian Journal of Human Genetics ◽

10.4103/0971-6866.142879 ◽

2014 ◽

Vol 20 (2) ◽

pp. 148 ◽

Cited By ~ 20

Author(s):

RameshR Chaudhari ◽

MS Dahiya

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Gujarat State ◽

Autosomal Short Tandem Repeats ◽

Short Tandem

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Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

10.21203/rs.3.rs-41454/v1 ◽

2020 ◽

Author(s):

Missa Millogo ◽

Serge Theophile Soubeiga ◽

Bapio Valerie Jean Telesphore Bazie ◽

Theodora Mahoukede Zohoncon ◽

Albert Theophane Yonli ◽

...

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

High Performance ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Repeat Analysis ◽

Paternity Index ◽

Genetic Analyzer ◽

Short Tandem

Abstract Background: the establishment of filiation by the current ABO, HLA, MNS, Kells and serum tests, pose a real reliability problem. It is then necessary to combine these methods with or to use high-performance methods such as microsatellite genetic analysis or short tandem repeats. This study aimed to compare the short tandem repeat technique with ABO/Rhesus system in combination with electrophoresis of hemoglobin. Methods: Fourteen (14) contested paternity trios were investigated. Blood samples were collected to determine blood groups using the Beth-Vincent method and the type of hemoglobin by electrophoresis. Blood spots on FTA paper were used for the analysis of 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, Amel) by capillary electrophoresis on the ABI 31310 Genetic Analyzer. The generated sequences were analyzed with GeneMapper® software version 3.2.1. The data were analyzed to determine the paternity index and the probability of paternity. Results: Of the fourteen (14) trios studied, ten (10) cases were probable inclusion, three (03) cases were exclusion and one (01) case was an undetermined paternity outcome with the ABO-Rhesus/ electrophoresis of hemoglobin system. While the analysis of genetic polymorphisms in DNA gave five (05) inclusions versus nine (09) exclusions of paternity. Of the 10 probable inclusion cases given by the ABO-Rhesus/Electrophoresis of hemoglobin system, only 05 cases (50%) were confirmed for inclusion by Short tandem repeat analysis. Conclusion: The analysis of short tandem repeat with sixteen genetic markers is more reliable in determining paternity than ABO-Rhesus/hemoglobin electrophoresis techniques.

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Genetic diversity and haplotype analysis of Guizhou Miao identified with 19 X-chromosomal short tandem repeats

International Journal of Legal Medicine ◽

10.1007/s00414-018-1871-z ◽

2018 ◽

Vol 133 (1) ◽

pp. 99-101 ◽

Cited By ~ 10

Author(s):

Yanyan Han ◽

Guanglin He ◽

Sheng Gong ◽

Junying Chen ◽

Zhigang Jiang ◽

...

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Haplotype Analysis ◽

Tandem Repeats ◽

Short Tandem

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Genetic diversity of 12 X-chromosomal short tandem repeats in Jewish populations

Forensic Science International Genetics Supplement Series ◽

10.1016/j.fsigss.2015.09.130 ◽

2015 ◽

Vol 5 ◽

pp. e327-e329 ◽

Cited By ~ 1

Author(s):

J.F. Ferragut ◽

J.A. Castro ◽

C. Ramon ◽

A. Picornell

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Short Tandem ◽

Jewish Populations

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Disputed paternity presumption in Burkina Faso: determination of the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00221-3 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Missa Millogo ◽

Serge Theophile Soubeiga ◽

Bapio Valerie Jean Telesphore Bazie ◽

Theodora Mahoukede Zohoncon ◽

Abdoul Karim Ouattara ◽

...

Keyword(s):

Burkina Faso ◽

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Str Analysis ◽

Resource Limited ◽

Biological Fathers ◽

Short Tandem

Abstract Background In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. The limitations of these tests in cases of disputed paternity require the use of Short Tandem Repeats (STR) for their certification. This study aimed to determine the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays in Burkina Faso, West Africa. Results Of the fourteen trios studied, the ABO-rhesus/hemoglobin electrophoresis analysis revealed ten probable inclusion cases, three exclusion cases, and one undetermined paternity. DNA STR analysis found five inclusions of paternity out of the ten probable inclusions with ABO-rhesus/hemoglobin electrophoresis assay versus nine exclusions of paternity. Conclusion This study showed that the implementation of the analysis of short tandem repeat is required to resolve increasing disputed filiation cases in Burkina Faso.

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Marginal diversity analysis of conservation of Chinese domestic duck breeds

Scientific Reports ◽

10.1038/s41598-019-49652-6 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Yang Zhang ◽

Laidi Wang ◽

Youqing Bian ◽

Zhaoshan Wang ◽

Qi Xu ◽

...

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Diversity Analysis ◽

Muscovy Duck ◽

Extinction Rate ◽

Domestic Duck ◽

Conservation Priority ◽

Contribution Rate ◽

Short Tandem

Abstract The present study aimed to systematically evaluate the genetic diversity of Chinese domestic duck breeds and ensure the most effective allocation and usage of conservation funds. We first performed an analysis of DNA genetic distance in 21 duck breeds by measuring short tandem repeats. Then, we calculated the extinction probability, contribution rate, and marginal diversity for each breed. The results showed that the extinction rate of the Zhongshan duck, Guangxi duck, and Ji’an duck were the highest at 0.67, 0.59, and 0.59, respectively, and that of the Linwu duck, Jinding duck, and Gaoyou duck were the lowest at 0.15, 0.18, and 0.19, respectively. The current diversity of populations was 7.72 and the expected diversity in five hundred years is 5.14 ± 1.15. The marginal diversity of the Chinese Muscovy duck was the largest (−2.20), accounting for 42.61% of the expected diversity, followed by the Guangxi duck (−0.49, 9.44%), whereas the Jinding duck was the smallest (−0.12; 2.32%). The protection potency of the Chinese Muscovy duck was the largest (0.61), followed by Guangxi duck (0.29), whereas the Jinding duck was the smallest (0.02). This study provides a reference for determining the conservation priority of Chinese domestic duck breeds or genetic resources.

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Genetic diversity of 17 Y-short tandem repeats in Indian population

Forensic Science International Genetics ◽

10.1016/j.fsigen.2010.12.007 ◽

2011 ◽

Vol 5 (4) ◽

pp. 363-367 ◽

Cited By ~ 15

Author(s):

Tania Ghosh ◽

D. Kalpana ◽

Sanjukta Mukerjee ◽

Meeta Mukherjee ◽

Anil Kumar Sharma ◽

...

Keyword(s):

Genetic Diversity ◽

Short Tandem Repeats ◽

Indian Population ◽

Tandem Repeats ◽

Short Tandem

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Efficiency of Tetrameric Short Tandem Repeats in Prestice Black-Pied Pig for Traceability and Parentity Testing

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201664020557 ◽

2016 ◽

Vol 64 (2) ◽

pp. 557-565

Author(s):

Irena Vrtková ◽

Štěpán Vrtek ◽

Lenka Falková

Keyword(s):

Information Content ◽

Polymorphism Information Content ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Genetic Resource ◽

Marker Panel ◽

Str Loci ◽

Related Individuals ◽

Crossbred Pigs ◽

Short Tandem

The main goal of the research was to evaluate suitability of 11 tetrameric STRs (Short Tandem Repeats) marker panel for the Prestice Black-Pied pig (PC) breed as the only genetic resource in the Czech Republic.The analysis was carried out in 522 breeding and slaughter PC individuals. We observed 94 alleles overall across whole 11 STR panel. The observed heterozygosityHOwas 0.677, the polymorphism information content (PIC) was in average 0.664 per locus. The probability of identity of two independent samples (PI) using all 11 STR loci was 4.037·10−11and the probability of identity related individuals (PISibs) was 8.315·10−5. The power of exclusion for loci combinations when both parents are known (P1), when only one of the parent is known (P2) and for two putative parents (P3) were 0.9996, 0.9899 and 0.9999. The efficiency of the 11 tetrameric STRs (Animaltype Pig kit) is higher in PC in comparison to commercial breeds and slaughter crossbred pigs. In genetic resource PC, the 11 STRs panel is usable for forensic purpose such parentity testing and traceability.

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