Disputed paternity presumption in Burkina Faso: determination of the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays

Abstract Background In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. The limitations of these tests in cases of disputed paternity require the use of Short Tandem Repeats (STR) for their certification. This study aimed to determine the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays in Burkina Faso, West Africa. Results Of the fourteen trios studied, the ABO-rhesus/hemoglobin electrophoresis analysis revealed ten probable inclusion cases, three exclusion cases, and one undetermined paternity. DNA STR analysis found five inclusions of paternity out of the ten probable inclusions with ABO-rhesus/hemoglobin electrophoresis assay versus nine exclusions of paternity. Conclusion This study showed that the implementation of the analysis of short tandem repeat is required to resolve increasing disputed filiation cases in Burkina Faso.

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Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

10.21203/rs.3.rs-41454/v1 ◽

2020 ◽

Author(s):

Missa Millogo ◽

Serge Theophile Soubeiga ◽

Bapio Valerie Jean Telesphore Bazie ◽

Theodora Mahoukede Zohoncon ◽

Albert Theophane Yonli ◽

...

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

High Performance ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Repeat Analysis ◽

Paternity Index ◽

Genetic Analyzer ◽

Short Tandem

Abstract Background: the establishment of filiation by the current ABO, HLA, MNS, Kells and serum tests, pose a real reliability problem. It is then necessary to combine these methods with or to use high-performance methods such as microsatellite genetic analysis or short tandem repeats. This study aimed to compare the short tandem repeat technique with ABO/Rhesus system in combination with electrophoresis of hemoglobin. Methods: Fourteen (14) contested paternity trios were investigated. Blood samples were collected to determine blood groups using the Beth-Vincent method and the type of hemoglobin by electrophoresis. Blood spots on FTA paper were used for the analysis of 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, Amel) by capillary electrophoresis on the ABI 31310 Genetic Analyzer. The generated sequences were analyzed with GeneMapper® software version 3.2.1. The data were analyzed to determine the paternity index and the probability of paternity. Results: Of the fourteen (14) trios studied, ten (10) cases were probable inclusion, three (03) cases were exclusion and one (01) case was an undetermined paternity outcome with the ABO-Rhesus/ electrophoresis of hemoglobin system. While the analysis of genetic polymorphisms in DNA gave five (05) inclusions versus nine (09) exclusions of paternity. Of the 10 probable inclusion cases given by the ABO-Rhesus/Electrophoresis of hemoglobin system, only 05 cases (50%) were confirmed for inclusion by Short tandem repeat analysis. Conclusion: The analysis of short tandem repeat with sixteen genetic markers is more reliable in determining paternity than ABO-Rhesus/hemoglobin electrophoresis techniques.

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5364759 DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats

Biotechnology Advances ◽

10.1016/0734-9750(96)83483-5 ◽

1996 ◽

Vol 14 (1) ◽

pp. 98

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Dna Typing ◽

Short Tandem

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Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2005.027 ◽

2005 ◽

Vol 43 (2) ◽

Author(s):

Giuseppe Calcagno ◽

Giuseppe Labruna ◽

Lucia Sacchetti

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Southern Italy ◽

Population Sample ◽

Lack Of Information ◽

Pcr Multiplex ◽

Y Haplotype ◽

Short Tandem

AbstractGiven the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex reactions: multiplex A for loci

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Global non-random abundance of short tandem repeats in rodents and primates.

10.1101/2021.11.24.469825 ◽

2021 ◽

Author(s):

Masoud Arabfard ◽

Mahmood Salesi ◽

Yazdan Hassani Nourian ◽

Iman Arabipour ◽

Ali Mohammad Ali Maddi ◽

...

Keyword(s):

Hierarchical Clustering ◽

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Whole Genome ◽

Random Coincidence ◽

Olive Baboon ◽

Model Study ◽

Short Tandem

Background: While of predominant abundance across vertebrate genomes and significant biological implications, the relevance of short tandem repeat (STR) abundance to speciation remains largely elusive and attributed to random coincidence for the most part. In a model study, here we collected whole-genome abundance of mono-, di-, and trinucleotide STRs in nine species, encompassing rodents and primates, including rat, mouse, olive baboon, gelada, macaque, gorilla, chimpanzee, bonobo, and human. The obtained unnormalized and normalized data were used to analyze hierarchical clustering of the STR abundances in the selected species. Results: We found massive differential abundances between the rodent and primate orders. In addition, while numerous STRs had random abundance across the nine selected species, the global abundance conformed to three consistent <clusters>, as follows: <rat, mouse>, <gelada, macaque, olive baboon>, <gorilla, chimpanzee, bonobo, human>, which coincided with the phylogenetic distances of the selected species (p< 4E-05). Exceptionally, in the trinucleotide STR compartment, human was significantly distant from all other species. Conclusion: We propose that the global abundance of STRs is non-random in rodents and primates, and probably had a determining impact on the speciation of the two orders. We also propose the STRs and STR lengths which specifically coincided with the phylogeny of the selected species.

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5364759 DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats

Biotechnology Advances ◽

10.1016/0734-9750(96)83416-1 ◽

1996 ◽

Vol 14 (1) ◽

pp. 81

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Dna Typing ◽

Short Tandem

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Primate Speciation Links to Massive and Directional Shrinkage of the Dinucleotide Short Tandem Repeat Compartment.

10.21203/rs.3.rs-114156/v1 ◽

2021 ◽

Author(s):

Masoud Arabfard ◽

Mahmood Salesi ◽

Iman Arabipour ◽

Reza Najafipour ◽

Ahmad Delbari ◽

...

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Great Apes ◽

Primate Species ◽

Evolutionary Trend ◽

Random Evolution ◽

Short Tandem

Abstract The evolutionary trend of short tandem repeats (STRs) at the crossroads of speciation remains largely elusive and attributed to random evolution for the most part. Here we investigated the dinucleotide STR compartment in primate speciation. We selected six species, which shared sequential chronological ancestors, including mouse, macaque, gorilla, chimpanzee, bonobo, and human, and collected three sets of data on the abundance of all classes of dinucleotide STRs (≥6-repeats) for three regions of every chromosome, each region spanning 10 Mb of DNA. In all three datasets, we found consistent directional shrinkage of the dinucleotide STR compartment in all the primate species selected vs. mouse, as follows: mouse>macaque>great apes. The >20-repeat STRs were the most significantly affected as a result of this shrinkage. We propose that massive and directional shrinkage of the dinucleotide STR compartment had a decisive link with primate speciation. This is a prime instane of massive directional STR trend in multiple speciation.

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Evolution of Old World monkeys and great apes links to massive and directional shrinkage of the dinucleotide short tandem repeat compartment

10.21203/rs.3.rs-441796/v1 ◽

2021 ◽

Author(s):

M Arabfard ◽

M Salesi ◽

I Arabipour ◽

M Ohadi

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Great Apes ◽

Old World Monkeys ◽

Evolutionary Trend ◽

Random Evolution ◽

Old World ◽

Short Tandem

Abstract Background: The evolutionary trend of short tandem repeats (STRs) at the crossroads of speciation remains largely elusive and attributed to random evolution for the most part. To explore this trend, we selected nine species, which shared sequential chronological ancestors, including rat, mouse, olive baboon, gelada, macaque, gorilla, chimpanzee, bonobo, and human, and collected three sets of data on the abundance of all classes of dinucleotide STRs (≥6-repeats) for three regions of every chromosome, each region spanning 10 Mb of DNA. Results: In all three datasets, we found directional shrinkage of the dinucleotide STR compartment as follows: rodents>Old World monkeys>great apes (P=0.000). The decremented gradient observed for the dinucleotide STRs was not detected for a number of other classes of STRs, such as mono and trinucleotide STRs. Conclusion: We report the first instance of massive and directional gradient of STRs, which may link with the evolution of Old World monkeys and great apes.

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