Reliability of muscle blood flow and oxygen consumption response from exercise using near-infrared spectroscopy

We applied near-infrared spectroscopy (NIRS) for the simultaneous measurement of forearm blood flow (FBF) and oxygen consumption (VO2) in the human by inducing a 50-mmHg venous occlusion. Eleven healthy subjects were studied both at rest and after hand exercise during vascular occlusion. FBF was also measured by strain-gauge plethysmography. FBF measured by NIRS was 1.9 +/- 0.8 ml.100 ml-1.min-1 at rest and 8.2 +/- 2.9 ml.100 ml-1.min-1 after hand exercise. These values showed a correlation (r = 0.94) with those obtained by the plethysmography. VO2 values were 4.6 +/- 1.3 microM O2 x 100 ml-1.min-1 at rest and 24.9 +/- 11.2 microM O2 x 100 ml-1.min-1 after hand exercise. The scatter of the FBF and VO2 values showed a good correlation between the two variables (r = 0.93). The results demonstrate that NIRS provides the particular advantage of obtaining the contemporary evaluation of blood flow and VO2, allowing correlation of these two variables by a single maneuver without discomfort for the subject.

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Blood flow and oxygen consumption with near-infrared spectroscopy and venous occlusion: spatial maps and the effect of time and pressure of inflation

Journal of Biomedical Optics ◽

10.1117/1.429995 ◽

2000 ◽

Vol 5 (3) ◽

pp. 269 ◽

Cited By ~ 39

Author(s):

Claudia Casavola ◽

Lelia Adelina Paunescu ◽

Sergio Fantini ◽

Enrico Gratton

Keyword(s):

Blood Flow ◽

Oxygen Consumption ◽

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Venous Occlusion ◽

Spatial Maps

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Near infrared spectroscopy-guided exercise training for claudication in peripheral arterial disease

European Journal of Preventive Cardiology ◽

10.1177/2047487318795192 ◽

2018 ◽

Vol 26 (5) ◽

pp. 471-480 ◽

Cited By ~ 4

Author(s):

Jonathan R Murrow ◽

Jared T Brizendine ◽

Bill Djire ◽

Hui-Ju Young ◽

Stephen Rathbun ◽

...

Keyword(s):

Blood Flow ◽

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Muscle Blood Flow ◽

Arterial Disease ◽

Walking Program ◽

Peripheral Arterial ◽

Walking Time ◽

Mitochondrial Capacity

Rationale Supervised treadmill exercise for claudication in peripheral arterial disease is effective but poorly tolerated because of ischemic leg pain. Near infrared spectroscopy allows non-invasive detection of muscle ischemia during exercise, allowing for characterization of tissue perfusion and oxygen utilization during training. Objective We evaluated walking time, muscle blood flow, and muscle mitochondrial capacity in patients with peripheral artery disease after a traditional pain-based walking program and after a muscle oxygen-guided walking program. Method and results Patients with peripheral artery disease trained thrice weekly in 40-minute-long sessions for 12 weeks, randomized to oxygen-guided training ( n = 8, age 72 ± 9.7 years, 25% female) versus traditional pain-based training ( n = 10, age 71.6 ± 8.8 years, 20% female). Oxygen-guided training intensity was determined by maintaining a 15% reduction in skeletal muscle oxygenation by near infrared spectroscopy rather than relying on symptoms of pain to determine exercise effort. Pain free and maximal walking times were measured with a 12-minute Gardner treadmill test. Gastrocnemius mitochondrial capacity and blood flow were measured using near infrared spectroscopy. Baseline pain-free walking time was similar on a Gardner treadmill test (2.5 ± 0.9 vs. 3.6 ± 1.0 min, p = 0.5). After training, oxygen-guided cohorts improved similar to pain-guided cohorts (pain-free walking time 6.7 ± 0.9 vs. 6.9 ± 1.1 min, p < 0.01 for change from baseline and p = 0.97 between cohorts). Mitochondrial capacity improved in both groups but more so in the pain-guided cohort than in the oxygen-guided cohort (38.8 ± 8.3 vs. 14.0 ± 9.3, p = 0.018). Resting muscle blood flow did not improve significantly in either group with training. Conclusions Oxygen-guided exercise training improves claudication comparable to pain-based training regimens. Adaptations in mitochondrial function rather than increases in limb perfusion may account for functional improvement. Increases in mitochondrial oxidative capacity may be proportional to the degree of tissue hypoxia during exercise.

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Measurement of Respiratory Muscle Blood Flow in Humans Using Near Infrared Spectroscopy and Indocyanine Green

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000323639.53977.27 ◽

2008 ◽

Vol 40 (Supplement) ◽

pp. S304

Author(s):

Jordan A. Guenette ◽

Ioannis Vogiatzis ◽

Spyros Zakythinos ◽

Dimitrios Athanasopoulos ◽

Spyretta Golemati ◽

...

Keyword(s):

Blood Flow ◽

Infrared Spectroscopy ◽

Indocyanine Green ◽

Near Infrared Spectroscopy ◽

Respiratory Muscle ◽

Near Infrared ◽

Muscle Blood Flow

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Reproducibility of near-infrared spectroscopy measurements of oxidative function and postexercise recovery kinetics in the medial gastrocnemius muscle

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2013-0347 ◽

2014 ◽

Vol 39 (5) ◽

pp. 521-529 ◽

Cited By ~ 29

Author(s):

William M. Southern ◽

Terence E. Ryan ◽

Mary A. Reynolds ◽

Kevin McCully

Keyword(s):

Blood Flow ◽

Oxygen Consumption ◽

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Near Infrared ◽

Plantar Flexion ◽

Medial Gastrocnemius ◽

Muscle Oxygen ◽

Oxidative Function ◽

Mitochondrial Capacity

The purpose of this study was to assess the reproducibility of resting blood flow, resting oxygen consumption, and mitochondrial capacity in skeletal muscle using near-infrared spectroscopy (NIRS). We also determined the influence of 2 exercise modalities (ergometer and rubber exercise bands) on the NIRS measurements. Fifteen young, healthy participants (5 female, 10 male) were tested on 2 nonconsecutive occasions within an 8-day period. The NIRS device was placed on the medial gastrocnemius. Venous and arterial occlusions were performed to obtain blood flow and oxygen consumption. A series of repeated arterial occlusions was used to measure the recovery kinetics of muscle oxygen consumption after ∼7–10 s of voluntary plantar flexion exercise. Resting blood flow had mean coefficients of variation (CV) of 42% and 38% for bands and ergometer, respectively, and resting metabolism had mean CVs of 17% and 12% for bands and ergometer, respectively. The recovery time constant of oxygen consumption (day 1 bands and ergometer: 23.2 ± 3.7 s, 27.6 ± 6.5 s, respectively; day 2 bands and ergometer: 25.5 ± 5.4 s, 25.0 ± 4.9 s, respectively) had mean CVs of 10% and 11% for bands and ergometer, respectively. We conclude that measurements of oxygen consumption and mitochondrial capacity using NIRS can be obtained with good reproducibility.

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