scholarly journals Nucleotide binding by actomyosin as a determinant of relaxation kinetics of rabbit phasic and tonic smooth muscle.

1996 ◽  
Vol 492 (3) ◽  
pp. 669-673 ◽  
Author(s):  
A S Khromov ◽  
A V Somlyo ◽  
A P Somlyo
2012 ◽  
Vol 465 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Hongqiu Guo ◽  
Renjian Huang ◽  
Shingo Semba ◽  
Jolanta Kordowska ◽  
Yang Hoon Huh ◽  
...  

1995 ◽  
Vol 268 (1) ◽  
pp. C14-C20 ◽  
Author(s):  
G. Hoffmann ◽  
Y. Ko ◽  
A. Sachinidis ◽  
B. O. Gobel ◽  
H. Vetter ◽  
...  

The kinetic properties of Na+/H+ exchange were investigated in vascular smooth muscle cells (VSMC) in culture from normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Antiport activity was measured in 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein-loaded cells after nigericin-induced cytosolic acidification. Studies were performed without (control) and with pretreatment of the cells with phorbol 12-myristate 13-acetate (PMA; 200 nM). Na+/H+ exchange markedly differed between the two strains with lower Hill coefficients [1.56 +/- 0.17 (SE) vs. 2.62 +/- 0.36] and higher maximal activity (Vmax) values (55.85 +/- 5.24 vs. 31.11 +/- 2.38 mmol H+.l-1.min-1) in SHR compared with WKY cell lines. PMA markedly altered the antiport kinetics in WKY VSMC with a decrease in the Hill coefficient (1.75 +/- 0.14) without affecting Vmax (31.88 +/- 1.55 mmol H+.l-1.min-1). In VSMC from SHR, PMA had no effect on the kinetic variables investigated. Thus two kinetic abnormalities are present with respect to Na+/H+ antiport activity in VSMC from SHR compared with WKY, i.e., increased Vmax and decreased Hill coefficient. The observation that PMA does not affect the kinetics of the Na+/H+ antiport in VSMC from SHR suggests a marked degree of antiporter prestimulation in this animal model of genetic hypertension.


2002 ◽  
Vol 106 (36) ◽  
pp. 9410-9417 ◽  
Author(s):  
T. D. Le ◽  
U. Olsson ◽  
H. Wennerström ◽  
P. Uhrmeister ◽  
B. Rathke ◽  
...  

2015 ◽  
Vol 17 (38) ◽  
pp. 25114-25122 ◽  
Author(s):  
Feng Wei ◽  
Wei Xiong ◽  
Wenhui Li ◽  
Wangting Lu ◽  
Heather C. Allen ◽  
...  

Polarization and frequency resolved SFG-VS to distinguish the head/tail groups of lipids, to resolve the assembly and relaxation kinetics of monolayers.


2011 ◽  
Vol 670 (1) ◽  
pp. 266-271 ◽  
Author(s):  
Claudia Ertl ◽  
Robert Lukowski ◽  
Katja Sigl ◽  
Jens Schlossmann ◽  
Franz Hofmann ◽  
...  
Keyword(s):  

1998 ◽  
Vol 57 (19) ◽  
pp. 12369-12377 ◽  
Author(s):  
K. El Sayed ◽  
J. A. Kenrow ◽  
C. J. Stanton

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