Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscle

We studied pre- and postnatal changes in total creatine kinase (CK) activity, mitochondrial creatine kinase (Mi-CK) activity and immunochemical reactivity with anti-Mi-CK antibodies in skeletal muscle specimens from 12 infants, 10 of them preterm born, after a pregnancy varying between 28 and 40 weeks. Our results demonstrate that Mi-CK is present in fetal human quadriceps muscle and that the specific activity of Mi-CK increases during prenatal development from week 28 to 40 by a factor about two. Generally, adult levels have not been reached at birth, indicating a further postnatal increase of the activity of the enzyme. The Mi-CK protein content also increases during prenatal development. These results suggest that in human skeletal muscle the expression and accumulation of Mi-CK starts at mid-gestation, later than is known to occur for cytosolic CK.

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The Use of Creatine Kinase Activity as an Index of Skeletal-Muscle Differentiation

Biochemical Society Transactions ◽

10.1042/bst0060509 ◽

1978 ◽

Vol 6 (3) ◽

pp. 509-511 ◽

Cited By ~ 9

Author(s):

GLENN E. MORRIS

Keyword(s):

Skeletal Muscle ◽

Creatine Kinase ◽

Kinase Activity ◽

Muscle Differentiation ◽

Creatine Kinase Activity ◽

Skeletal Muscle Differentiation

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Mitochondrial creatine kinase activity in patients with disturbed energy generation in muscle mitochondria

Journal of Inherited Metabolic Disease ◽

10.1007/bf00735397 ◽

1994 ◽

Vol 17 (1) ◽

pp. 67-73 ◽

Cited By ~ 3

Author(s):

J. Smeitink ◽

W. Ruitenbeek ◽

R. Sengers ◽

R. Wevers ◽

T. v. Lith ◽

...

Keyword(s):

Creatine Kinase ◽

Kinase Activity ◽

Energy Generation ◽

Creatine Kinase Activity ◽

Mitochondrial Creatine Kinase ◽

Muscle Mitochondria

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Effect of the Inhibition of Mitochondrial Creatine Kinase Activity on the Clinical Diagnosis of Suspected Acute Myocardial Infarction

The Open Clinical Chemistry Journal ◽

10.2174/1874241601205010001 ◽

2012 ◽

Vol 5 (1) ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Masato Maekawa

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Creatine Kinase ◽

Clinical Diagnosis ◽

Kinase Activity ◽

Creatine Kinase Activity ◽

Mitochondrial Creatine Kinase ◽

Suspected Acute Myocardial Infarction

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Creatine kinase:aspartate aminotransferase activity ratio as an indicator of the source of an increased creatine kinase activity.

Clinical Chemistry ◽

10.1093/clinchem/34.12.2506 ◽

1988 ◽

Vol 34 (12) ◽

pp. 2506-2510 ◽

Cited By ~ 5

Author(s):

D R Dufour

Keyword(s):

Myocardial Infarction ◽

Skeletal Muscle ◽

Acute Myocardial Infarction ◽

Creatine Kinase ◽

Retrospective Study ◽

Kinase Activity ◽

Activity Ratio ◽

Creatine Kinase Activity ◽

Aminotransferase Activity ◽

Creatine Kinase Isoenzyme

Abstract Although measurements of creatine kinase isoenzyme 2 (CK-MB) are often used to diagnose acute myocardial infarction, their sensitivity and specificity are less than 100%. Because skeletal muscle contains more CK and less aspartate aminotransferase (AST) than cardiac muscle, the CK/AST ratio might provide a useful adjunct in evaluating the source of a supranormal value for CK. I established the following decision levels in a retrospective study of 342 patients: ratios less than 14 (if total CK was 300-1200 U/L), less than 20 (CK 1201-2000 U/L), or less than 25 (CK greater than 2000 U/L) suggested myocardial infarction, with a sensitivity of 95% and a specificity of 65%. In a validation study with 277 additional patients, liver disease and alcohol abuse caused erroneous results, leading to exclusion of 22% of these patients. In the remaining cases, sensitivity was 94%, specificity 90%. The CK/AST ratios changed little with time, suggesting that a single value would be adequate for evaluating patients with increased CK.

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