Single-Cell Analysis Using Hyperspectral Imaging Modalities

Almost a decade ago, hyperspectral imaging (HSI) was employed by the NASA in satellite imaging applications such as remote sensing technology. This technology has since been extensively used in the exploration of minerals, agricultural purposes, water resources, and urban development needs. Due to recent advancements in optical re-construction and imaging, HSI can now be applied down to micro- and nanometer scales possibly allowing for exquisite control and analysis of single cell to complex biological systems. This short review provides a description of the working principle of the HSI technology and how HSI can be used to assist, substitute, and validate traditional imaging technologies. This is followed by a description of the use of HSI for biological analysis and medical diagnostics with emphasis on single-cell analysis using HSI.

Download Full-text

Intracellular Thermal Sensor for Single Cell Analysis -Short review

Jurnal Teknologi ◽

10.11113/jt.v73.4409 ◽

2015 ◽

Vol 73 (6) ◽

Cited By ~ 1

Author(s):

Salma Abdullah Binslem ◽

Mohd Ridzuan Ahmad ◽

Zubaidah Awang

Keyword(s):

Single Cell ◽

Single Cell Analysis ◽

Near Field ◽

Short Review ◽

Environmental Variable ◽

System Level ◽

Far Field ◽

Cell Analysis ◽

Field Methods ◽

Excessive Heat

Temperature is a key environmental variable that affects almost all natural and engineered systems from the system level down to the molecular level. The first attempt to measure temperature goes back to 1592 when Galileo Galilei tried to develop a thermometer. Since then having accurate temperature measurements has been a challenging research topic. Recently, in single cell analysis, internal temperature and heat generation inside a living cell has proven to have important roles in the survival of cells, controls many cellular activities for instance; cell division and gene expression. Moreover, cancerous cells are identified with excessive heat production. Studies have been done by researchers from different fields in the attempt to develop sensors that can accurately report the temperature inside living cells. This short review presents the most recent developments in nanoscale thermometry for biological applications, highlighting the recent advances in the near field and the far field methods. The far field thermometry cover sensors that depend on the luminescence’s of the material, for example: quantum dots, nanoparticles, and fluorescents based compounds. While, near field thermometry is based on different principles depending on the sensing mechanism used. Some of the examples mentioned are thermocouple thermometry, RNA thermometry, resonant thermometry, photoacoustic thermometry and carbon nanotubes thermometry.

Download Full-text

Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Biosensors ◽

10.3390/bios11110470 ◽

2021 ◽

Vol 11 (11) ◽

pp. 470

Author(s):

Zhao Zhang ◽

Xiaowen Huang ◽

Ke Liu ◽

Tiancong Lan ◽

Zixin Wang ◽

...

Keyword(s):

Single Cell ◽

Electrical Impedance ◽

Single Cell Analysis ◽

Single Cells ◽

Clinical Diagnostics ◽

Cell Analysis ◽

Electrical Parameters ◽

Impedance Sensing ◽

Recent Advances ◽

Sensing Technology

Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.

Download Full-text