scholarly journals Analysis of Vibrio cholerae O1 Isolated in Japan by Pulsed-field Gel Electrophoresis

1998 ◽  
Vol 72 (6) ◽  
pp. 575-584
Author(s):  
Yotaku GYOBU ◽  
Shiho HOSOROGI ◽  
Toshio SHIMADA
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vibrio Cholerae O1

scholarly journals Pulsed-Field Gel Electrophoresis-Based Molecular Comparison of Vibrio cholerae O1 Isolates from Domestic and Imported Cases of Cholera in Japan

2000 ◽  
Vol 38 (1) ◽  
pp. 424-426
Author(s):  
Eiji Arakawa ◽  
Toshiyuki Murase ◽  
Shigeru Matsushita ◽  
Toshio Shimada ◽  
Shiro Yamai ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Imported Cases ◽  
Molecular Comparison ◽  
Pulse Type ◽  
Vibrio Cholerae O1 ◽  
El Tor

ABSTRACT Sixty-seven Vibrio cholerae O1 El Tor isolates (36 domestic and 31 imported) were classified into 19 subtypes by Not I- and Sfi I-digested pulsed-field gel electrophoresis. Twenty-five of 36 domestic and 4 imported isolates were assigned to a Not I-A1– Sfi I-A1 subtype, suggesting that this pulse type is widely distributed in Asia and Japan.

scholarly journals Vibrio cholerae El Tor strains linked to global cholera are homogeneous by pulsed-field gel electrophoresis

2021 ◽  
Author(s):  
Fatema-Tuz Johura ◽  
Sahitya Ranjan Biswas ◽  
Shah M Rashed ◽  
Mohammad Tarequl Islam ◽  
Saiful Islam ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Homogeneous Cluster ◽  
Vibrio Cholerae O1 ◽  
Spatio Temporal ◽  
Pandemic Strains ◽  
El Tor

Vibrio cholerae O1 El Tor, causative agent of the ongoing seventh cholera pandemic, is native to the aquatic environment of the Ganges Delta, Bay of Bengal (GDBB). Recent studies traced pandemic strains to the GDBB and proposed global spread of cholera had occurred via intercontinental transmission. In the research presented here, Not I-digested genomic DNA extracted from V. cholerae O1 clinical and environmental strains isolated in Bangladesh during 2004 – 2014 was analyzed by pulsed-field gel electrophoresis (PFGE). Results of cluster analysis showed 94.67% of the V. cholerae isolates belonged to clade A and included the majority of clinical isolates of spatio-temporal origin and representing different cholera endemic foci. The rest of the strains were estuarine, all environmental isolates from Mathbaria, Bangladesh, and occurred as singletons, clustered in clades B and C, or in the small clades D and E. Cluster analysis of the Bangladeshi strains and including 157 El Tor strains from thirteen countries in Asia, Africa, and the Americas revealed 85% of the total set of isolates belonged to clade A, indicating all were related, yet did not form an homogeneous cluster. Overall, 15% of the global strains comprised multiple small clades or segregated as singletons. Three sub-clades could be discerned within the major clade A, reflecting distinct lineages of V. cholerae El Tor associated with cholera in Asia, Africa, and the Americas. The presence in Asia and the Americas of non-pandemic V. cholerae El Tor populations differing by PFGE and from strains associated with cholera globally suggests different ecotypes are resident in distant geographies.

Vibrio cholerae O139 Bengal isolated from India, Bangladesh and Thailand are clonal as determined by pulsed-field gel electrophoresis

1994 ◽  
Vol 29 (1) ◽  
pp. 109-110 ◽  
Author(s):  
Hisao Kurazono ◽  
Jun Okuda ◽  
Yoshifumi Takeda ◽  
G. Balakrish Nair ◽  
M.J. Albert ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vibrio Cholerae O139

scholarly journals Phenotypic and Genotypic Characteristics and Epidemiological Significance of ctx+ Strains of Vibrio cholerae Isolated from Seafood in Malaysia

2004 ◽  
Vol 70 (4) ◽  
pp. 1964-1972 ◽  
Author(s):  
Chien-Hsien Chen ◽  
Toshio Shimada ◽  
Nasreldin Elhadi ◽  
Son Radu ◽  
Mitsuaki Nishibuchi
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Pcr Ribotyping ◽  
Electrophoresis Method ◽  
Toxigenic Strains ◽  
Arbitrarily Primed Pcr ◽  
Genotypic Characteristics

ABSTRACT Of 97 strains of Vibrio cholerae isolated from various seafoods in Malaysia in 1998 and 1999, 20 strains carried the ctx gene and produced cholera toxin. Fourteen, one, and five of these toxigenic strains belonged to the O139, O1 Ogawa, and rough serotypes, respectively. The rough strains had the rfb gene of the O1 serotype. The toxigenic strains varied in their biochemical characteristics, the amount of cholera toxin produced, their antibiograms, and the presence or absence of the pTLC plasmid sequence. DNA fingerprinting analysis by arbitrarily primed PCR, ribotyping, and a pulsed-field gel electrophoresis method classified the toxigenic strains into 3, 7, and 10 types, respectively. The relatedness of these toxigenic strains to clinical strains isolated in other countries and from international travelers was examined by using a dendrogram constructed from the pulsed-field gel electrophoresis profiles. The results of the examination of the antibiogram and the possession of the toxin-linked cryptic plasmid were consistent with the dendrogram-based relatedness: the O139 strains isolated from Malaysian seafoods could be separated into two groups that appear to have been introduced from the Bengal area independently. The rough strains of Malaysian seafood origin formed one group and belonged to a cluster unique to the Thailand-Malaysia-Laos region, and this group may have persisted in this area for a long period. The single O1 Ogawa strain detected in Malaysian seafood appears to have an origin and route of introduction different from those of the O139 and the rough strains.

scholarly journals Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

2000 ◽  
Vol 38 (11) ◽  
pp. 4249-4253 ◽  
Author(s):  
Pallavi Garg ◽  
Ranjan K. Nandy ◽  
Papiya Chaudhury ◽  
Nandini Roy Chowdhury ◽  
Keya De ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Vibrio Cholerae ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Pulsed Field Gel Electrophoresis ◽  
Vibrio Cholerae O1 ◽  
El Tor ◽  
Restriction Fragment Length

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular characterization by ribotyping, restriction fragment length polymorphism, and pulsed-field gel electrophoresis indicated that recent Inaba strains are remarkably different from the earlier Inaba strains but are very similar to the prevailing V. choleraeO1 Ogawa El Tor biotype strains. The antibiograms of the Inaba strains were also similar to those of the recent V. cholerae Ogawa strains. These V. cholerae O1 Inaba strains appear to have evolved from the currently prevailing Ogawa strains and are likely to dominate in the coming years.

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