Proof-of-concept demonstration of a miniaturized three-channel multiresolution imaging system

2014 ◽  
Author(s):  
Gebirie Y. Belay ◽  
Heidi Ottevaere ◽  
Youri Meuret ◽  
Michael Vervaeke ◽  
Jürgen Van Erps ◽  
...  
Keyword(s):  
Imaging System ◽  
Proof Of Concept ◽  
Multiresolution Imaging

scholarly journals Proof of concept of a multimodal intravital molecular imaging system for tumour transpathology investigation

2021 ◽  
Author(s):  
Zhen Liu ◽  
Tao Cheng ◽  
Stephan Düwel ◽  
Ziying Jian ◽  
Geoffrey J. Topping ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Imaging System ◽  
Ex Vivo ◽  
Fiducial Markers ◽  
Proof Of Concept ◽  
Registration Accuracy ◽  
Microscopic Imaging ◽  
Window Chamber

Abstract Background Transpathology highlights the interpretation of the underlying physiology behind molecular imaging. However, it remains challenging due to the discrepancies between in vivo and in vitro measurements and difficulties of precise co-registration between trans-scaled images. This study aims to develop a multimodal intravital molecular imaging (MIMI) system as a tool for in vivo tumour transpathology investigation. Methods The proposed MIMI system integrates high-resolution positron imaging, magnetic resonance imaging (MRI) and microscopic imaging on a dorsal skin window chamber on an athymic nude rat. The window chamber frame was designed to be compatible with multimodal imaging and its fiducial markers were customized for precise physical alignment among modalities. The co-registration accuracy was evaluated based on phantoms with thin catheters. For proof of concept, tumour models of the human colorectal adenocarcinoma cell line HT-29 were imaged. The tissue within the window chamber was sectioned, fixed and haematoxylin–eosin (HE) stained for comparison with multimodal in vivo imaging. Results The final MIMI system had a maximum field of view (FOV) of 18 mm × 18 mm. Using the fiducial markers and the tubing phantom, the co-registration errors are 0.18 ± 0.27 mm between MRI and positron imaging, 0.19 ± 0.22 mm between positron imaging and microscopic imaging and 0.15 ± 0.27 mm between MRI and microscopic imaging. A pilot test demonstrated that the MIMI system provides an integrative visualization of the tumour anatomy, vasculatures and metabolism of the in vivo tumour microenvironment, which was consistent with ex vivo pathology. Conclusions The established multimodal intravital imaging system provided a co-registered in vivo platform for trans-scale and transparent investigation of the underlying pathology behind imaging, which has the potential to enhance the translation of molecular imaging.

Proof-of-concept demonstration of a miniaturized multi-resolution refocusing imaging system using an electrically tunable lens

2014 ◽  
Author(s):  
L. Smeesters ◽  
G. Y. Belay ◽  
H. Ottevaere ◽  
Y. Meuret ◽  
Michael Vervaeke ◽  
...  
Keyword(s):  
Imaging System ◽  
Proof Of Concept ◽  
Tunable Lens

scholarly journals Fast NO<sub>2</sub> retrievals from Odin-OSIRIS limb scatter measurements

2011 ◽  
Vol 4 (5) ◽  
pp. 965-972 ◽  
Author(s):  
A. E. Bourassa ◽  
C. A. McLinden ◽  
C. E. Sioris ◽  
S. Brohede ◽  
A. F. Bathgate ◽  
...  
Keyword(s):  
Infrared Imaging ◽  
Imaging System ◽  
Computational Cost ◽  
Data Sets ◽  
Proof Of Concept ◽  
Spectral Fitting ◽  
Analysis Technique ◽  
Order Of Magnitude ◽  
Very High ◽  
Good Agreement

Abstract. The feasibility of retrieving vertical profiles of NO2 from space-based measurements of limb scattered sunlight has been demonstrated using several different data sets since the 1980's. The NO2 data product routinely retrieved from measurements made by the Optical Spectrograph and InfraRed Imaging System (OSIRIS) instrument onboard the Odin satellite uses a spectral fitting technique over the 437 to 451 nm range, over which there are 36 individual wavelength measurements. In this work we present a proof of concept technique for the retrieval of NO2 using only 4 of the 36 OSIRIS measurements in this wavelength range, which reduces the computational cost by almost an order of magnitude. The method is an adaptation of a triplet analysis technique that is currently used for the OSIRIS retrievals of ozone at Chappuis band wavelengths. The results obtained are shown to be in very good agreement with the spectral fit method, and provide an important alternative for applications where the computational burden is very high. Additionally this provides a baseline for future instrument design in terms of cost effectiveness and reducing spectral range requirements.

scholarly journals Fast NO<sub>2</sub> retrievals from Odin-OSIRIS limb scatter measurements

2010 ◽  
Vol 3 (6) ◽  
pp. 5499-5519
Author(s):  
A. E. Bourassa ◽  
C. A. McLinden ◽  
C. E. Sioris ◽  
S. Brohede ◽  
E. J. Llewellyn ◽  
...  
Keyword(s):  
Infrared Imaging ◽  
Imaging System ◽  
Computational Cost ◽  
Data Sets ◽  
Proof Of Concept ◽  
Spectral Fitting ◽  
Analysis Technique ◽  
Order Of Magnitude ◽  
Very High ◽  
Good Agreement

Abstract. The feasibility of retrieving vertical profiles of NO2 from space-based measurements of limb scattered sunlight has been demonstrated using several different data sets since the 1980's. The NO2 data product routinely retrieved from measurements made by the Optical Spectrograph and InfraRed Imaging System (OSIRIS) instrument onboard the Odin satellite uses a spectral fitting technique over the 437 to 451 nm range, over which there are 36 individual wavelength measurements. In this work we present a proof of concept technique for the retrieval of NO2 using only 4 of the 36 OSIRIS measurements in this wavelength range, which reduces the computational cost by almost an order of magnitude. The method is an adaptation of a triplet analysis technique that is currently used for the OSIRIS retrievals of ozone at Chappuis band wavelengths. The results obtained are shown to be in very good agreement with the spectral fit method, and provide an important alternative for two dimensional tomographic algorithms where the computational burden is very high. Additionally this provides a baseline for future instrument design in terms of cost effectiveness and boosting signal to noise by reducing spectral resolution requirements.

Breast imaging using an LED-based photoacoustic and ultrasound imaging system: a proof-of-concept study

2021 ◽  
Author(s):  
Mithun Kuniyil Ajith Singh ◽  
Maura Dantuma ◽  
Francis Kalloor Joseph ◽  
Srirang Manohar ◽  
Wiendelt Steenbergen
Keyword(s):  
Ultrasound Imaging ◽  
Breast Imaging ◽  
Imaging System ◽  
Proof Of Concept ◽  
Concept Study ◽  
System A

scholarly journals D3D augmented reality imaging system: proof of concept in mammography

2016 ◽  
Vol Volume 9 ◽  
pp. 277-283 ◽  
Author(s):  
David Douglas ◽  
Emanuel Petricoin ◽  
Lance Liotta ◽  
Eugene Wilson
Keyword(s):  
Augmented Reality ◽  
Imaging System ◽  
Proof Of Concept

Demonstration of a multichannel, multiresolution imaging system

2013 ◽  
Vol 52 (24) ◽  
pp. 6081 ◽  
Author(s):  
Gebirie Y. Belay ◽  
Heidi Ottevaere ◽  
Youri Meuret ◽  
Michael Vervaeke ◽  
Jurgen Van Erps ◽  
...  
Keyword(s):  
Imaging System ◽  
Multiresolution Imaging

scholarly journals Low-cost Kinect Version 2 imaging system for breath hold monitoring and gating: Proof of concept study for breast cancer VMAT radiotherapy

2018 ◽  
Vol 19 (3) ◽  
pp. 71-78 ◽  
Author(s):  
David M. Edmunds ◽  
Lone Gothard ◽  
Komel Khabra ◽  
Anna Kirby ◽  
Poonam Madhale ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Imaging System ◽  
Low Cost ◽  
Breath Hold ◽  
Proof Of Concept ◽  
Concept Study

scholarly journals Design of a Multimodal Imaging System and Its First Application to Distinguish Grey and White Matter of Brain Tissue. A Proof-of-Concept-Study

2021 ◽  
Vol 11 (11) ◽  
pp. 4777
Author(s):  
Annabell Heintz ◽  
Sebastian Sold ◽  
Felix Wühler ◽  
Julia Dyckow ◽  
Lucas Schirmer ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
White Matter ◽  
Brain Tissue ◽  
Multimodal Imaging ◽  
Imaging System ◽  
Incident Light ◽  
Transmitted Light ◽  
Polarization Microscopy ◽  
Proof Of Concept ◽  
Light Mode

Multimodal imaging gains increasing popularity for biomedical applications. This article presents the design of a novel multimodal imaging system. The centerpiece is a light microscope operating in the incident and transmitted light mode. Additionally, Raman spectroscopy and VIS/NIR reflectance spectroscopy are adapted. The proof-of-concept is realized to distinguish between grey matter (GM) and white matter (WM) of normal mouse brain tissue. Besides Raman and VIS/NIR spectroscopy, the following optical microscopy techniques are applied in the incident light mode: brightfield, darkfield, and polarization microscopy. To complement the study, brightfield images of a hematoxylin and eosin (H&E) stained cryosection in the transmitted light mode are recorded using the same imaging system. Data acquisition based on polarization microscopy and Raman spectroscopy gives the best results regarding the tissue differentiation of the unstained section. In addition to the discrimination of GM and WM, both modalities are suited to highlight differences in the density of myelinated axons. For Raman spectroscopy, this is achieved by calculating the sum of two intensity peak ratios (I2857 + I2888)/I2930 in the high-wavenumber region. For an optimum combination of the modalities, it is recommended to apply the molecule-specific but time-consuming Raman spectroscopy to smaller regions of interest, which have previously been identified by the microscopic modes.

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