A chemist’s view of inflammation in contusion injured spinal cord in a rat model: noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury

2019 ◽  
Author(s):  
Seth Fillioe ◽  
Kyle K. Bishop ◽  
Sai Han Myo Tun ◽  
Paul Dent ◽  
Bin Deng ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Raman Spectroscopy ◽  
Rat Model ◽  
Injured Spinal Cord ◽  
In Vivo Raman Spectroscopy

scholarly journals A Collagen-Based Scaffold for Promoting Neural Plasticity in a Rat Model of Spinal Cord Injury

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2245
Author(s):  
Jue-Zong Yeh ◽  
Ding-Han Wang ◽  
Juin-Hong Cherng ◽  
Yi-Wen Wang ◽  
Gang-Yi Fan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Rat Model ◽  
Neural Plasticity ◽  
Collagen Scaffold ◽  
Glial Scar ◽  
Beneficial Effects ◽  
Cord Injury

In spinal cord injury (SCI) therapy, glial scarring formed by activated astrocytes is a primary problem that needs to be solved to enhance axonal regeneration. In this study, we developed and used a collagen scaffold for glial scar replacement to create an appropriate environment in an SCI rat model and determined whether neural plasticity can be manipulated using this approach. We used four experimental groups, as follows: SCI-collagen scaffold, SCI control, normal spinal cord-collagen scaffold, and normal control. The collagen scaffold showed excellent in vitro and in vivo biocompatibility. Immunofluorescence staining revealed increased expression of neurofilament and fibronectin and reduced expression of glial fibrillary acidic protein and anti-chondroitin sulfate in the collagen scaffold-treated SCI rats at 1 and 4 weeks post-implantation compared with that in untreated SCI control. This indicates that the collagen scaffold implantation promoted neuronal survival and axonal growth within the injured site and prevented glial scar formation by controlling astrocyte production for their normal functioning. Our study highlights the feasibility of using the collagen scaffold in SCI repair. The collagen scaffold was found to exert beneficial effects on neuronal activity and may help in manipulating synaptic plasticity, implying its great potential for clinical application in SCI.

In vivo Raman spectroscopy of breast tumors prephotodynamic and postphotodynamic therapy

2018 ◽  
Vol 49 (5) ◽  
pp. 786-791 ◽  
Author(s):  
Tanmoy Bhattacharjee ◽  
Leticia C. Fontana ◽  
Leandro Raniero ◽  
Juliana Ferreira-Strixino
Keyword(s):  
Raman Spectroscopy ◽  
Breast Tumors ◽  
In Vivo Raman Spectroscopy

Detection of skin lactate by in vivo Raman spectroscopy

1999 ◽  
pp. 485-486
Author(s):  
Peter J. Caspers ◽  
Gerald W. Lucassen ◽  
Hajo A. Bruining ◽  
Gerwin J. Puppels
Keyword(s):  
Raman Spectroscopy ◽  
In Vivo Raman Spectroscopy

scholarly journals Overexpression of Rictor in the injured spinal cord promotes functional recovery in a rat model of spinal cord injury

2020 ◽  
Vol 34 (5) ◽  
pp. 6984-6998
Author(s):  
Ningning Chen ◽  
Pengxiang Zhou ◽  
Xizhe Liu ◽  
Jiachun Li ◽  
Yong Wan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Rat Model ◽  
Injured Spinal Cord ◽  
Cord Injury

MRI as an imaging tool for in vivo noninvasive morphological and (partially) functional examination of injured spinal cord

2020 ◽  
pp. 49-66
Author(s):  
Fano Molinaro ◽  
Pier Luigi La Zazzera ◽  
Marilena Ferraris ◽  
Giulia Morbidoni ◽  
Domenico Zaca’ ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Injured Spinal Cord ◽  
Imaging Tool ◽  
Functional Examination

In vivo Raman spectroscopy study on the stimulation mechanism of surfactant

2020 ◽  
Vol 26 (6) ◽  
pp. 898-904
Author(s):  
Yifan He ◽  
Wenhai Wu ◽  
Jing Li ◽  
Youting Liu ◽  
Zhaohui Qu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Spectroscopy Study ◽  
In Vivo Raman Spectroscopy

scholarly journals Microstructure and Mechanical Properties of PU/PLDL Sponges Intended for Grafting Injured Spinal Cord

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2693
Author(s):  
Anna Lis-Bartos ◽  
Dariusz Szarek ◽  
Małgorzata Krok-Borkowicz ◽  
Krzysztof Marycz ◽  
Włodzimierz Jarmundowicz ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Spinal Cord ◽  
Young Modulus ◽  
In Vivo Studies ◽  
Polymer Composition ◽  
In Vitro Toxicity ◽  
Injured Spinal Cord ◽  
Degradation Rates

Highly porous, elastic, and degradable polyurethane and polyurethane/polylactide (PU/PLDL) sponges, in various shapes and sizes, with open interconnected pores, and porosity up to 90% have been manufactured. They have been intended for gap filling in the injured spinal cord. The porosity of the sponges depended on the content of polylactide, i.e., it decreased with the increase of polylactide content. The rise of polylactide content caused an increase of Young modulus and rigidity as well as a more complex morphology of the polyurethane/polylactide blends. The mechanical properties, in vitro toxicity, and degradation in artificial cerebrospinal fluid were tested. Sponges underwent continuous degradation with varying degradation rates depending on the polymer composition. In vitro cell studies with fibroblast cultures proved the biocompatibility of the polymers. Based on the obtained results, the designed PU/PLDL sponges appeared to be promising candidates for bridging gaps within injured spinal cord in further in vitro and in vivo studies.

Transcutaneous in vivo Raman spectroscopy of breast tumors and pretumors

2015 ◽  
Vol 46 (11) ◽  
pp. 1053-1061 ◽  
Author(s):  
T. Bhattacharjee ◽  
G. Maru ◽  
A. Ingle ◽  
C. Murali Krishna
Keyword(s):  
Raman Spectroscopy ◽  
Breast Tumors ◽  
In Vivo Raman Spectroscopy

Feature engineering applied to intraoperative in vivo Raman spectroscopy sheds light on molecular processes in brain cancer: a retrospective study of 65 patients

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6517-6532 ◽  
Author(s):  
Émile Lemoine ◽  
Frédérick Dallaire ◽  
Rajeev Yadav ◽  
Rajeev Agarwal ◽  
Samuel Kadoury ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Retrospective Study ◽  
Brain Cancer ◽  
New Method ◽  
Feature Engineering ◽  
Molecular Processes ◽  
System P ◽  
In Vivo Raman Spectroscopy

A new method to improve the statistical interpretability of biological Raman Spectroscopy was applied to spectra acquired in vivo during neurosurgical resection of brain cancer, revealing oncogenic processes captured by the Raman system.

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