Phase-sensitive flow cytometry: fluorescence lifetime-based sensing technology for analyzing free fluorophore and cells/particles labeled with fluorescent probes

1999 ◽  
Author(s):  
John A. Steinkamp
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Lifetime ◽  
Fluorescent Probes ◽  
Sensing Technology ◽  
Phase Sensitive

Analysis of fluorescence lifetime and quenching of FITC-conjugated antibodies on cells by phase-sensitive flow cytometry

Cytometry ◽  
1996 ◽  
Vol 25 (3) ◽  
pp. 271-279 ◽  
Author(s):  
C. Deka ◽  
B. E. Lehnert ◽  
N. M. Lehnert ◽  
G. M. Jones ◽  
L. A. Sklar ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Lifetime ◽  
Phase Sensitive

scholarly journals Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry

Cytometry ◽  
2003 ◽  
Vol 52A (1) ◽  
pp. 46-55 ◽  
Author(s):  
H. Helen Cui ◽  
Joseph G. Valdez ◽  
John A. Steinkamp ◽  
Harry A. Crissman
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Lifetime ◽  
Dna And Rna ◽  
Phase Sensitive ◽  
Cellular Dna

scholarly journals Monitoring of a Highly Flexible Aircraft Model Wing Using Time-Expanded Phase-Sensitive OTDR

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3766
Author(s):  
Miguel Soriano-Amat ◽  
David Fragas-Sánchez ◽  
Hugo F. Martins ◽  
David Vallespín-Fontcuberta ◽  
Javier Preciado-Garbayo ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Fuel Efficiency ◽  
Optical Fiber Sensor ◽  
High Sensitivity ◽  
Time Domain Reflectometry ◽  
Structural Deformation ◽  
Flexible Wings ◽  
Sensing Technology ◽  
Distributed Optical Fiber Sensor ◽  
Phase Sensitive

In recent years, the use of highly flexible wings in aerial vehicles (e.g., aircraft or drones) has been attracting increasing interest, as they are lightweight, which can improve fuel-efficiency and distinct flight performances. Continuous wing monitoring can provide valuable information to prevent fatal failures and optimize aircraft control. In this paper, we demonstrate the capabilities of a distributed optical fiber sensor based on time-expanded phase-sensitive optical time-domain reflectometry (TE-ΦOTDR) technology for structural health monitoring of highly flexible wings, including static (i.e., bend and torsion), and dynamic (e.g., vibration) structural deformation. This distributed sensing technology provides a remarkable spatial resolution of 2 cm, with detection and processing bandwidths well under the MHz, arising as a novel, highly efficient monitoring methodology for this kind of structure. Conventional optical fibers were embedded in two highly flexible specimens that represented an aircraft wing, and different bending and twisting movements were detected and quantified with high sensitivity and minimal intrusiveness.

scholarly journals Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry

2013 ◽  
Vol 4 (8) ◽  
pp. 1390 ◽  
Author(s):  
Ali Vaziri Gohar ◽  
Ruofan Cao ◽  
Patrick Jenkins ◽  
Wenyan Li ◽  
Jessica P. Houston ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Subcellular Localization ◽  
Fluorescence Lifetime ◽  
Time Resolved ◽  
Egfp Fluorescence

Fluorescence-lifetime-based phase-sensitive sensor array with LED or diode laser excitation for chemical- and biosensing

1999 ◽  
Author(s):  
Emmanuil M. Rabinovich ◽  
Michael J. O'Brien II ◽  
A. A. Ukhanov ◽  
Ravinder K. Jain ◽  
Victor H. Perez-Luna ◽  
...  
Keyword(s):  
Diode Laser ◽  
Fluorescence Lifetime ◽  
Sensor Array ◽  
Laser Excitation ◽  
Sensitive Sensor ◽  
Phase Sensitive
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