Kinetics of photo processes in dye molecules and the resolution ofa two-photon excitation fluorescence microscope with nanosecond laser

2000 ◽  
Author(s):  
Evgeny Kleymionov ◽  
Valery S. Ivanov ◽  
Niko Meltola ◽  
Pekka E. Hanninen ◽  
Erkki J. Soini
Keyword(s):  
Fluorescence Microscope ◽  
Nanosecond Laser ◽  
Dye Molecules ◽  
Two Photon ◽  
Photon Excitation ◽  
Kinetics Of ◽  
Two Photon Excitation

Reaction kinetics of a two-photon excitation microparticle based immunoassay—from modelling to practice

2003 ◽  
Vol 105 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Pekka Hänninen ◽  
Matti Waris ◽  
Mika Kettunen ◽  
Erkki Soini
Keyword(s):  
Reaction Kinetics ◽  
Two Photon ◽  
Photon Excitation ◽  
Kinetics Of ◽  
Two Photon Excitation

Photobleaching Kinetics of Optically Trapped Multilamellar Vesicles Containing Verteporfin Using Two-photon Excitation§

2006 ◽  
Vol 82 (1) ◽  
pp. 152 ◽  
Author(s):  
Kimberley S. Samkoe ◽  
Matthew S. Fecica ◽  
Rebecca L. Goyan ◽  
Jennifer L. Buchholz ◽  
Chantel Campbell ◽  
...  
Keyword(s):  
Multilamellar Vesicles ◽  
Two Photon ◽  
Photon Excitation ◽  
Optically Trapped ◽  
Kinetics Of ◽  
Two Photon Excitation

Investigation of kinetics of mid-infrared ASEs of Xe by two photon excitation

2021 ◽  
pp. 118630
Author(s):  
Junzhi Chu ◽  
Ke Huang ◽  
Shu Hu ◽  
Jinbo Liu ◽  
Ying Chen ◽  
...  
Keyword(s):  
Mid Infrared ◽  
Two Photon ◽  
Photon Excitation ◽  
Kinetics Of ◽  
Two Photon Excitation

Orientation of the AD-1 azo-dye molecules in solid nanostructured films upon the two-photon excitation

Laser Physics ◽  
2008 ◽  
Vol 18 (12) ◽  
pp. 1400-1410
Author(s):  
S. A. Magnitskiy ◽  
N. M. Nagorskiy ◽  
V. M. Kozenkov
Keyword(s):  
Azo Dye ◽  
Dye Molecules ◽  
Nanostructured Films ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two‐photon excitation spectroscopy and excited state decay kinetics of isolated diphenylbutadiene

1985 ◽  
Vol 83 (5) ◽  
pp. 2186-2190 ◽  
Author(s):  
James S. Horwitz ◽  
Bryan E. Kohler ◽  
Thomas A. Spiglanin
Keyword(s):  
Excited State ◽  
Decay Kinetics ◽  
Two Photon ◽  
State Decay ◽  
Photon Excitation ◽  
Kinetics Of ◽  
Two Photon Excitation

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

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