Cytological effect of laser-irradiated peanut seeds and induction of mutation

Larvae of the flesh-fly, Sarcophaga bullata, were injected with the synthetic moulting hormone ecdysterone or saline at the beginning of the third and final larval instar. One group was left untreated. The ecdysterone-injected larvae showed an increase in number of secondary lysosomes in the midgut epithelial cells similar to that observed at the onset of metamorphosis, an event which would normally occur about 48 hr later in these larvae.

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Cytological effect of Tilt 250 EC in the successive phases of the Triticale rust disease process caused by Puccinia recondita f. sp. tritici

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1997.019 ◽

2014 ◽

Vol 66 (2) ◽

pp. 153-158

Author(s):

Krystyna Brzezicka-Szymczyk ◽

Władysław Golinowski ◽

Czesław Zamorski

Keyword(s):

Disease Process ◽

Puccinia Recondita ◽

Ergosterol Biosynthesis ◽

Developmental Cycle ◽

Chemical Agent ◽

Rust Disease ◽

Cell Wall Modification ◽

Cytological Effect ◽

Irregular Growth ◽

Cell Collapse

The disease process caused by rust (Puccinia recondita f. sp. tritici) in Triticale (Triticale-Wittmack cv. Bolero) and the effect of the application of the propikonazole - Tilt 250 EC are described. In plants not protected chemically one could observe the whole pathogen developmental cycle ending with the appearance of uredinia with urediniospores. The highest pathogen susceptibility to chemical agent was observed at the time of inoculation, during incubation and at the beginning of the actual disease. By inhibiting the ergosterol biosynthesis in the fungus cells the preparation (propikonazole) inhibited the development of the intra- and extracellular mycelium. The mycelium degeneration manifested itself by the irregular growth of intercellular hyphae, perforation of septa, homogenization of protoplasts and cell collapse. The thickening of the cell wall, modification of the perihaustorial space and protoplast obliteration were observed in the haustoria.

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Cytological Effects of Leaf Extract of Artemisia vulgaris L. on Meristematic Cells of Allium cepa L.

Our Nature ◽

10.3126/on.v10i1.7787 ◽

2013 ◽

Vol 10 (1) ◽

pp. 242-248 ◽

Cited By ~ 3

Author(s):

P.D. Rai ◽

N. Paudel ◽

S.R. Shakya

Keyword(s):

Allium Cepa ◽

Leaf Extract ◽

Chromosomal Abnormalities ◽

Water Extract ◽

Inhibitory Effect ◽

Artemisia Vulgaris ◽

Active Components ◽

Duration Of Treatment ◽

Cytological Effect ◽

Allium Cepa L

Artemisia vulgaris L., a perennial aromatic shrub with bitter taste, is considered as a medicinal plant and water extract of it consists of active components like psilostachyin A, psilostachy C, Exiguaflavanone A, Maackiain, fernenol with both anti-bacterial and medicinal value. It has been used to perserve crops and stored grains both in liquid and powder by farmers. The cytological effect was observed in 5, 7.5, 10 and 20% concentrations of Leaf Extract of Artemisia vulgaris (LEA) on Allium cepa L. in five different time durations. The present study showed the correlation between concentration of LEA and duration of treatment in controlled state. Higher concentrations of LEA as well as longer duration of treatment generate the inhibitory effect on the division of cells. Lower concentration of LEA showed less effect on Mitotic Index value (MI) in comparison with control value, which is attributed to delay in metabolic activities. It also showed the change in phase indices and induced chromosomal abnormalities among the phases.DOI: http://dx.doi.org/10.3126/on.v10i1.7787

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Methylene-blue/Coriphosphine-O/Acridine-Orange, chromosomal DNA and visible light: Interaction and cytological effect in Drosophila melanogaster

Genetica ◽

10.1007/bf00127203 ◽

1981 ◽

Vol 55 (3) ◽

pp. 203-207 ◽

Cited By ~ 3

Author(s):

R. Mezzanotte ◽

L. Ferrucci ◽

A. Marchi

Keyword(s):

Methylene Blue ◽

Drosophila Melanogaster ◽

Visible Light ◽

Acridine Orange ◽

Chromosomal Dna ◽

Cytological Effect ◽

Light Interaction

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OBSERVATIONS ON THE INITIAL CYTOLOGICAL EFFECTS OF BACTERIOPHAGE INFECTION

Canadian Journal of Microbiology ◽

10.1139/m57-053 ◽

1957 ◽

Vol 3 (3) ◽

pp. 493-499 ◽

Cited By ~ 1

Author(s):

J. F. Whitfield ◽

R. G. E. Murray

Keyword(s):

Cell Structure ◽

Ionic Equilibrium ◽

Cytological Effect ◽

Bacteriophage Infection ◽

Phage Dna ◽

Deficient Medium ◽

Phage Multiplication ◽

Host Cell Surface ◽

Major Alteration ◽

Host Chromatin

Chromatin aggregation is the earliest cytological effect of several phage infections (e.g. P1, P2, and T7). This occurs at low multiplicities of infection if the medium contains a sufficient quantity of sodium chloride; on salt-deficient medium this aggregation no longer appears and is replaced by fragmentation. Infections with phages T2 and T5 do not normally result in the aggregation of the host chromatin, but this does occur when the multiplicity of infection is higher than three to six phages per cell and salt is present in the medium. The appearance of nuclear aggregation as the first cytological effect of infection under these circumstances adds an additional cytological stage. In the case of infection with T5 this is followed by chromatin disappearance, which is the normal first stage. Infections with T5, at high multiplicities, on salt-deficient agar cause chromatin fragmentation and no aggregation.Chromatin aggregation was again the major alteration in cell structure when T2 bacteriophage "ghosts" were adsorbed. Since injection of phage DNA and phage multiplication do not occur under these conditions, it is concluded that adsorption alone is sufficient to cause this effect.It is hypothesized that adsorption of phage alters the host cell surface so that the cell can no longer maintain an ionic equilibrium. The shift in internal ion balance is reflected in chromatin aggregation or fragmentation depending on the salt concentration of the medium.

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