scholarly journals Cytological Effects of Leaf Extract of Artemisia vulgaris L. on Meristematic Cells of Allium cepa L.

Our Nature ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 242-248 ◽  
Author(s):  
P.D. Rai ◽  
N. Paudel ◽  
S.R. Shakya
Keyword(s):  
Allium Cepa ◽  
Leaf Extract ◽  
Chromosomal Abnormalities ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Artemisia Vulgaris ◽  
Active Components ◽  
Duration Of Treatment ◽  
Cytological Effect ◽  
Allium Cepa L

Artemisia vulgaris L., a perennial aromatic shrub with bitter taste, is considered as a medicinal plant and water extract of it consists of active components like psilostachyin A, psilostachy C, Exiguaflavanone A, Maackiain, fernenol with both anti-bacterial and medicinal value. It has been used to perserve crops and stored grains both in liquid and powder by farmers. The cytological effect was observed in 5, 7.5, 10 and 20% concentrations of Leaf Extract of Artemisia vulgaris (LEA) on Allium cepa L. in five different time durations. The present study showed the correlation between concentration of LEA and duration of treatment in controlled state. Higher concentrations of LEA as well as longer duration of treatment generate the inhibitory effect on the division of cells. Lower concentration of LEA showed less effect on Mitotic Index value (MI) in comparison with control value, which is attributed to delay in metabolic activities. It also showed the change in phase indices and induced chromosomal abnormalities among the phases.DOI: http://dx.doi.org/10.3126/on.v10i1.7787

scholarly journals Study of Mitotic Activity and Chromosomal Behaviour in Root Meristem of Allium cepa L. Treated with Magnesium Sulphate

2009 ◽  
Vol 15 ◽  
pp. 83-88 ◽  
Author(s):  
Prabhakar Bhatta ◽  
S. R. Sakya
Keyword(s):  
Key Words ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Biological System ◽  
Magnesium Sulphate ◽  
Root Meristem ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Duration Of Treatment ◽  
Chromosomal Behaviour

The mitotic activity and chromosomal behaviour was observed in four different concentrations of magnesium sulphate at four different duration of treatment using Allium cepa as a biological system. Mito-inhibitory effect of the compound was observed in higher concentration. The compound was capable of producing various chromosomal abnormalities indicating the cytostatic and clastogenic properties of magnesium sulphate. Key words: Allium cepa, magnesium sulphate, mitotic index, phase indices.doi: 10.3126/eco.v15i0.1947 ECOPRINT 15: 83-88, 2008

scholarly journals Effects of L-lysine on cytogenetical and physiological parameters in Allium cepa L. under salt stress

2019 ◽  
Vol 48 (3) ◽  
pp. 625-632
Author(s):  
Dilek Çavuşoğlu
Keyword(s):  
Seed Germination ◽  
Allium Cepa ◽  
Seedling Growth ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Nacl Stress ◽  
Physiological Parameters ◽  
Root Tip ◽  
Allium Cepa L

The role of L-lysine (Lys) on some cytogenetic and physiological parameters in Allium cepa L. seeds exposed to salt was evaluated. NaCl stress on the other hand showed a significantly inhibitory effect on the seedling growth and seed germination of Allium cepa. Besides, it significantly reduced the mitotic index in the root tip meristems of seeds and increased micronuclei which are the most effective and simplest indicator of cytological damage and chromosomal abnormalities. However, the effects of salinity on chromosomal aberrations, seedling growth, seed germination and mitotic activity have decreased significantly with Lys application.

scholarly journals Effect of Chemical Mutagens on Chromosomal behavior of Allium cepa L

2018 ◽  
Vol 7 (4) ◽  
pp. 2202
Author(s):  
Jyotirmoy Sharma ◽  
Jinu Devi Rajkumari ◽  
Ingtipi W. ◽  
Rakesh Boro ◽  
Shivani Das
Keyword(s):  
Mitotic Index ◽  
Allium Cepa ◽  
Chromosomal Abnormalities ◽  
Root Tips ◽  
Duration Of Treatment ◽  
Chemical Mutagens ◽  
Increase With Increase ◽  
Allium Cepa L ◽  
Wide Range ◽  
Multinuclear Cells

Effect of chemical mutagens was studied in mitotic cells of Allium cepa L. For this purpose root tips were treated with saturated solution of peradichloro benzene, 0.2% EMS and 0.2 % freshly prepared colchicines for 4hours to 9hours.The studies revealed a wide range of chromosomal abnormalities such as stickiness, laggards, chromosomal bridge, unequal separation, nuclear budding, multinuclear cells etc.The parentage of chromosomal abnormalities in different mitotic stages were calculated on mitotic index, frequency of phases and percentage of abnormalities in mitosis. The chromosomal abnormalities increase with increase duration of treatment. The results showed that in colchicines and EMS more than 50% abnormalities ware observed after 8 hrs of treatment, while in PDB it takes 6 hours treatment. Minimum MI was recorded as 65% in controlled cells. In colchicines and EMS treated cells 0.9.5%, 20% MI ware observed after 8hrs of treatment. Whereas in PDB maximum 23% MI was recorded in cells treated for 6hrs.

scholarly journals Investigation of cytotoxic and mutagenic effects of Malpighia glabra L. (barbados cherry) fruit pulp and vitamin C on plant and animal test systems

2012 ◽  
Vol 32 (2) ◽  
pp. 405-411 ◽  
Author(s):  
Elisângela Düsman ◽  
Márcia Flores da Silva Ferreira ◽  
Alessandra Paim Berti ◽  
Rosinete Gonçalves Mariucci ◽  
Mário Sérgio Mantovani ◽  
...  
Keyword(s):  
Cell Division ◽  
Vitamin C ◽  
Allium Cepa ◽  
Wistar Rats ◽  
Chromosomal Abnormalities ◽  
Bone Marrow Cells ◽  
Recovery Period ◽  
Test System ◽  
Liquid Form ◽  
Allium Cepa L

Fruits are important sources of nutrients in human diet, and Barbados Cherry (Malpighia glabra L.) is of particular interest due to its high content of antioxidants. Diets rich in fruits and vegetables protect individuals against diseases and cancer, but excessive intake of vitamins may act as pro-oxidant and generate changes in DNA. To evaluate the effect of different in natura (BAN) and frozen (BAF) Barbados Cherry pulp concentrations and synthetic vitamin C in liquid form (VC) on the chromosome level and the cell cycle division, root meristeme cells of Allium cepa L. and bone marrow cells of Wistar rats Rattus norvegicus, were used as test system. In Allium cepa L., BAN, at the highest concentration (0.4 mg.mL-1) and BAF, at the lowest concentration (0.2 mg.mL-1), inhibited cell division, and there was recovery of cell division after the recovery period in water only for BAN. In the Wistar rats, all treatments with Barbados Cherry, either acute or subchronic, were not cytotoxic or mutagenic; only the highest concentration of VC increased significantly the rate of chromosomal abnormalities. The data obtained are important to reinforce the use of Barbados Cherry fruit in the diet.

scholarly journals Effects of Pre-Storage Treatment with Ethanol and CO2 on Onion Dormancy

1970 ◽  
Vol 15 ◽  
pp. 55-62 ◽  
Author(s):  
Altaf Qadir ◽  
Fumio Hashinaga ◽  
Md Rezaul Karim
Keyword(s):  
Adverse Effect ◽  
Chemical Analysis ◽  
Key Words ◽  
Allium Cepa ◽  
Inhibitory Effect ◽  
Storage Treatment ◽  
Allium Cepa L

Onion (Allium cepa L.) cvs. ‘Tazan' and ‘Iyomante' were pre-treated either with ethanol (at a dose of 0.34, 0.68 and 0.91g.kg-1) and stored at 0, 10, 20 and 30ºC or with 100% CO2 (for 3.5, 7 and 14 days) and stored at 20ºC. Treatment of onion with ethanol delayed rooting, sprouting and reduced decay in both cultivars. Chemical analysis and organoleptic tests showed that the treatments had no adverse effect on the quality of onion. Pre-storage treatment with 100% CO2 for 3.5 and 7 days enhanced rooting and sprouting, while 14-days treatment exhibited a slight inhibitory effect. Key words: Onion, storage, ethanol, CO2   doi: 10.3329/jbs.v15i0.2203 J. bio-sci. 15: 55-62, 2007

Mitotic Effect of Water Extract of Different Ipomoea Species on Allium cepa L.

2006 ◽  
Vol 9 (6) ◽  
pp. 1116-1120 ◽  
Author(s):  
A. Mondal ◽  
G. Kabir ◽  
N. Yasmin ◽  
A.M.S. Alam ◽  
H.A. Khatun
Keyword(s):  
Allium Cepa ◽  
Water Extract ◽  
Effect Of Water ◽  
Allium Cepa L ◽  
Ipomoea Species

Allelopathic activity of Mexican sunflower (Tithonia diversifolia) in soil

Weed Science ◽  
1998 ◽  
Vol 46 (4) ◽  
pp. 432-437 ◽  
Author(s):  
Suthep Tongma ◽  
Katsuichiro Kobayashi ◽  
Kenji Usui
Keyword(s):  
Root Growth ◽  
Plant Species ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Rice Seedling ◽  
Test Plant ◽  
Active Components ◽  
Allelopathic Activity ◽  
Phytotoxic Activity ◽  
Shoot And Root Growth

Laboratory experiments were undertaken to investigate the allelopathic activity of Mexican sunflower in soil as well as to determine the effect of several soil factors on activity. There was a decrease in shoot and root growth of the test plant species when grown in soil previously planted with Mexican sunflower. However, seed germination was not affected. Soil-water separated from the same soil also inhibited shoot and root growth. A water extract from Mexican sunflower leaves applied to soil also reduced shoot and root growth, but the degree of inhibition varied among test plant species. Incorporation of dry Mexican sunflower leaves into soil at the rate of 1 and 2% (w/w) inhibited the growth of rice seedlings. The phytotoxic activity of incorporated leaf residue diminished 4 wk after treatment of soil. The inhibitory effect of a leaf water extract in nonautoclaved soil was less than in autoclaved soil and the phytotoxic effect to rice seedling grown in autoclaved soil was less than in autoclaved sand. It is suggested that the decrease in allelopathic activity of Mexican sunflower extract and its residue in soil is due to the action of soil microorganisms and the soil adsorption of the active components.

scholarly journals Multi-protective role of Echinacea purpurea L. water extract in Allium cepa L. against mercury(II) chloride

2021 ◽  
Author(s):  
Emine Yalçın ◽  
Oksal Macar ◽  
Tuğçe Kalefetoğlu Macar ◽  
Dilek Çavuşoğlu ◽  
Kültiğin Çavuşoğlu
Keyword(s):  
Allium Cepa ◽  
Water Extract ◽  
Echinacea Purpurea ◽  
Protective Role ◽  
Allium Cepa L
Sign in / Sign up

Export Citation Format

Share Document