Hyperbaric oxygen therapy augments the photodynamic action of methylene blue against bacteria in vitro

2007 ◽  
Author(s):  
S. K. Bisland ◽  
F. N. Dadani ◽  
C. Chien ◽  
B. C. Wilson
Keyword(s):  
Methylene Blue ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Photodynamic Action

scholarly journals Research on the Possibility of Treating Streptococcal Infections with Hyperbaric Oxygenation

2020 ◽  
Vol 70 (1) ◽  
pp. 43-46
Author(s):  
Władysław Wolański
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Hyperbaric Oxygenation ◽  
Lethal Effect ◽  
Streptococcal Infections ◽  
Group A

AbstractThe aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.

Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures1

2020 ◽  
pp. 1-13
Author(s):  
P. Engel ◽  
M. Ranieri ◽  
O. Felthaus ◽  
S. Geis ◽  
F. Haubner ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cells ◽  
Wound Healing ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Reactive Oxygen ◽  
Adipose Derived Stem Cells ◽  
Oxygen Species

BACKGROUND: A key moderator of wound healing is oxygen. Wound healing is a dynamic and carefully orchestrated process involving blood cells, cytokines, parenchymal cells (i.e. fibroblasts and mesenchymal stem cells) and extracellular matrix reorganization. Human adipose derived stem cells as well as human fibroblasts produce soluble factors, exhibit diverse effects on inflammation and anti inflammation response and are involved in wound healing processes. Hyperbaric oxygen therapy is an effective adjunct treatment for ischemic disorders such as chronic infection or chronic wounds. In vitro effects of hyperbaric oxygen therapy on human cells were presented in many studies except for those on mono- and co-cultures of human adipose derived stem cells and fibroblasts. OBJECTIVE: The aim of this study was to investigate the effects of hyperbaric oxygen therapy on mono- and co-cultures of human adipose derived stem cells and fibroblasts. METHODS: Mono- and co-cultures from human adipose derived stem cells and fibroblasts were established. These cultures were exposed to hyperbaric oxygen therapy every 24 h for five consecutive days. Measuring experiments were performed on the first, third and fifth day. Therapy effects on the expression of VEGF, IL 6 and reactive oxygen species were investigated. RESULTS: After exposure to hyperbaric oxygen, cell culturess showed a significant increase in the expression of VEGF after 3 and 5 days. All cultures showed significantly reduced formation of reactive oxygen species throughout the experiments. The expression of IL-6 decreased during the experiment in mono-cultures of human adipose derived stem cells and co-cultures. In contrast, mono-cultures of human skin fibroblasts showed an overall significantly increased expression of IL-6. CONCLUSIONS: Hyperbaric oxygen therapy leads to immunmodulatory and proangiogenetic effects in a wound-like enviroment of adipose derived stem cells and fibroblasts.

The Role of β-Catenin Signaling Pathway on Proliferation of Rats Neural Stem Cells After Hyperbaric Oxygen Therapy In Vitro

2010 ◽  
Vol 31 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Xiao-Ying Zhang ◽  
Yu-Jia Yang ◽  
Pei-Ru Xu ◽  
Xiang-Rong Zheng ◽  
Qing-Hong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Signaling Pathway ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy

The effect of hyperbaric oxygen therapy on osteoblast activity in vitro

Bone ◽  
2012 ◽  
Vol 51 (6) ◽  
pp. S11
Author(s):  
H. Al Hadi ◽  
G. Smerdon ◽  
S. Fox
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Osteoblast Activity

scholarly journals Hyperbaric oxygen therapy and in vitro fertilization—a pilot study

2003 ◽  
Vol 80 ◽  
pp. 130-131
Author(s):  
Bradley J.Van Voorhis ◽  
J.E. Greensmith ◽  
Anuja Dokras ◽  
Michelle R. Maifeld ◽  
Amy E.T. Sparks ◽  
...  
Keyword(s):  
Pilot Study ◽  
In Vitro Fertilization ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Vitro Fertilization

Adjuvant Hyperbaric Oxygen Therapy in the Management of Crush Injury and Traumatic Ischemia: An Evidence-Based Approach

2005 ◽  
Vol 71 (2) ◽  
pp. 144-151 ◽  
Author(s):  
Lisardo Garcia-Covarrubias ◽  
Norman E. Mcswain ◽  
Keith Van Meter ◽  
Richard M. Bell
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Case Series ◽  
Crush Injury ◽  
Evidence Based ◽  
Class Iii

Hyperbaric oxygen therapy (HBO) has been recommended as an adjunct treatment in acute traumatic ischemia and crush injury. Several animal models have shown better outcomes when HBO is used in crush injury and compartment syndrome. Animal and in vitro models have suggested that these beneficial effects may be mediated by attenuation of ischemia-reperfusion injury. We did a systematic review of the literature using the Eastern Association for the Surgery of Trauma (EAST) recommendations for evidence-based reviews. An electronic search using Medline, OVID technologies, and the Cochrane database was performed. Only clinical papers published between 1966 and December 2003 with at least five patients that included enough information to evaluate were selected. A group of trauma experts reviewed the selected articles and scored them applying the instrument developed by the EAST practice management guidelines committee. Nine documents fulfilled the inclusion criteria for a total of approximately 150 patients. Most documents were retrospective, uncontrolled, and case series lacking a standardized methodology (class III). There was one prospective controlled randomized trial with some limitations on its design. We determined that eight of nine studies showed a beneficial effect from HBO with only one major complication. We concluded that adjunctive HBO is not likely to be harmful and could be beneficial if administered early. Well designed clinical studies are warranted.

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