Holographic Device For Analysis Of Objects Inaccessible By Direct Microscopic Technique

1986 ◽  
Author(s):  
J. Surget
Keyword(s):  
Microscopic Technique ◽  
Holographic Device

A Sensitive On-Grid Immunoelectron Microscopic Procedure for Diagnosis of Rotavirus Infection

1980 ◽  
Vol 38 ◽  
pp. 506-507
Author(s):  
M. F. Miller ◽  
A. R. Rubenstein
Keyword(s):  
Electron Microscopy ◽  
Specific Antibody ◽  
Immune Complexes ◽  
Acute Gastroenteritis ◽  
Plant Viruses ◽  
Aggregation Process ◽  
Microscopic Technique ◽  
Fecal Material ◽  
Present Communication ◽  
Number Of Particles

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.

scholarly journals Zn2+-Depletion Enhances Lysosome Fission in Cultured Rat Embryonic Cortical Neurons Revealed by a Modified Epifluorescence Microscopic Technique

2021 ◽  
pp. 1-5
Author(s):  
Hung-Chun Tsao ◽  
Yi-Feng Liao ◽  
Feby Wijaya Pratiwi ◽  
Chung-Yuan Mou ◽  
Yi-Jhen Lin ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Microscopic Technique

Abstract

scholarly journals Correction to: Network analysis of the left anterior descending coronary arteries in swim-trained rats by an in situ video microscopic technique

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Marianna Török ◽  
Petra Merkely ◽  
Anna Monori-Kiss ◽  
Eszter Mária Horváth ◽  
Réka Eszter Sziva ◽  
...  
Keyword(s):  
Network Analysis ◽  
Coronary Arteries ◽  
Microscopic Technique

scholarly journals Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.

1988 ◽  
Vol 36 (8) ◽  
pp. 991-995 ◽  
Author(s):  
L A Bryan ◽  
P J Griebel ◽  
D M Haines ◽  
W C Davis ◽  
J R Allen
Keyword(s):  
Monoclonal Antibody ◽  
Langerhans Cells ◽  
Staining Method ◽  
Class Ii ◽  
Immunocytochemical Staining ◽  
Microscopic Technique ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens ◽  
Class Ii Antigens

We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.

Authenticating and distinguishing the eight species of traditional Tibetan medicine “Meiduoluomi” by microscopic technique

2009 ◽  
Vol 72 (10) ◽  
pp. 727-736 ◽  
Author(s):  
Jing An ◽  
Jie Li ◽  
Jiang-Gang Wang ◽  
Zhi-Feng Zhang ◽  
Chu Chen ◽  
...  
Keyword(s):  
Tibetan Medicine ◽  
Microscopic Technique ◽  
Traditional Tibetan Medicine

Comparison of New Telescopic Video Microlaryngoscopic and Standard Microlaryngoscopic Techniques

1992 ◽  
Vol 101 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Eiji Yanagisawa ◽  
Ken Yanagisawa ◽  
Jay B. Horowitz ◽  
Lawrence J. Mambrino
Keyword(s):  
Video Camera ◽  
New Method ◽  
Depth Of Field ◽  
Microscopic Technique ◽  
New Approach ◽  
Advantages And Disadvantages ◽  
Laryngeal Surgery ◽  
Valuable Method ◽  
Microlaryngeal Surgery ◽  
Television Image

A new approach to microlaryngeal surgery using a specially designed video microlaryngoscope with a rigid endoscopic telescope and an attached video camera was introduced by Kantor et al in 1990. The ability to video document and perform surgery of the larynx by viewing a high-resolution television image was demonstrated. This method was recommended over the standard microscopic technique for increased visibility with greater depth of field, unimpeded instrument access, instant documentation, and superior teaching value. The authors tried this new method and the standard microscopic technique at the same sitting on a series of patients. This paper will compare these two different techniques and discuss their advantages and disadvantages. Although the new method has many advantages, the standard microscopic technique remains as a valuable method in laryngeal surgery.

On the toxicity of methylene blue

1927 ◽  
Vol 23 (11) ◽  
pp. 1091-1097
Author(s):  
A. I. Puchek
Keyword(s):  
Methylene Blue ◽  
Urinary Tract ◽  
19Th Century ◽  
Analgesic Effect ◽  
Nerve Tissue ◽  
Microscopic Technique ◽  
The 19Th Century

Methylene blue was discovered by the chemist Careau at the end of the 19th century. R. Koch, in 1882, first used it in microscopic technique, working with the tuberculosis pathogen he had discovered. Later, in 1890, Ehrlich noticed the affinity of methylene blue for nerve tissue and its analgesic effect. A year later it was suggested by Gutmann, and then by Giems for treatment of malaria. Since that time, blue has been used for the treatment of a wide variety of diseases and, as can be seen from the literature, with some success, for example, in diseases of the urinary tract of a gonorrheic nature, in dysentery, recurrent typhus, in tuberculosis of the throat, in neoplasms, nephritis of an infectious nature, etc.

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