A highly efficient system for wave number frequency analysis using an slv in an underwater housing

1999 ◽  
Vol 106 (4) ◽  
pp. 2292-2292
Author(s):  
Walter H. Boober ◽  
Phillip Abbott
Keyword(s):  
Frequency Analysis ◽  
Wave Number ◽  
Efficient System ◽  
Highly Efficient ◽  
Number Frequency

Turbulence functions useful for probes (space–time correlation) and for scattering (wave-number–frequency spectrum) analysis

1968 ◽  
Vol 46 (23) ◽  
pp. 2683-2702 ◽  
Author(s):  
I. P. Shkarofsky
Keyword(s):  
Characteristic Size ◽  
Time Correlation ◽  
Exponential Model ◽  
Time Correlation Function ◽  
Maximum Energy ◽  
Confluent Hypergeometric Function ◽  
Space Time ◽  
Wave Number ◽  
Taylor’S Hypothesis ◽  
Number Frequency

The wave-number–frequency dependent spectral function, S(k, ω), and the space–time correlation function, C(r, t), are considered in a turbulent flowing plasma. The decay mechanisms are associated with either velocity fluctuations about the mean convection velocity or diffusion effects or attachment, or combinations of these, including the Brownian motion model. The ψ(k, ω) function, which is the ratio of S(k, ω) to its frequency-integrated value, depends on the mechanism and exhibits a profile which can be Gaussian, Lorentzian, a Z function, a Hermite polynomial modification of the Gaussian, or a confluent hypergeometric function. Anisotropic forms are also considered.The function C(r, t), obtained by convolving ψ (r, t) with C(r), the space autocorrelation function, is next considered. Adopting a Gaussian or an exponential model (which may be anisotropic) for C(r), we illustrate C(r, t) forms, which can readily be manipulated. Furthermore, letting r = 0, we derive two conditions for the applicability of Taylor's hypothesis. The assumption of frozen flow is not necessary, only that the root-mean-square Lagrangian displacement in a given time, associated with the decay, be much smaller than both the flow distance and the characteristic size of blobs having maximum energy.

Abstract 1356: GENOCTC, a highly efficient system for enrichment of circulating tumor cells and its clinical application

2019 ◽  
Author(s):  
Miso Lee ◽  
Jiyeon Ryu ◽  
Hyeon Jin Kim ◽  
Dohyeong Kim ◽  
Mi Young Kim ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Application ◽  
Efficient System ◽  
Highly Efficient

Highly Efficient System To Deliver Taxanes into Tumor Cells: Docetaxel-Loaded Chitosan Oligomer Colloidal Carriers

2008 ◽  
Vol 9 (8) ◽  
pp. 2186-2193 ◽  
Author(s):  
Maria V. Lozano ◽  
Daniel Torrecilla ◽  
Dolores Torres ◽  
Anxo Vidal ◽  
Fernando Domínguez ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Efficient System ◽  
Highly Efficient ◽  
Chitosan Oligomer

Space-time-wave number-frequency Z(x,t,k,f) analysis of SAW generation on fluid filled cylindrical shells

Ultrasonics ◽  
2004 ◽  
Vol 42 (1-9) ◽  
pp. 383-389 ◽  
Author(s):  
Loı̈c Martinez ◽  
Bruno Morvan ◽  
Jean Louis Izbicki
Keyword(s):  
Cylindrical Shells ◽  
Space Time ◽  
Wave Number ◽  
Number Frequency

scholarly journals Efficient Reverse Genetics Reveals Genetic Determinants of Budding and Fusogenic Differences between Nipah and Hendra Viruses and Enables Real-Time Monitoring of Viral Spread in Small Animal Models of Henipavirus Infection

2014 ◽  
Vol 89 (2) ◽  
pp. 1242-1253 ◽  
Author(s):  
Tatyana Yun ◽  
Arnold Park ◽  
Terence E. Hill ◽  
Olivier Pernet ◽  
Shannon M. Beaty ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Reverse Genetics ◽  
Nipah Virus ◽  
Hendra Virus ◽  
Efficient System ◽  
Replicative Fitness ◽  
Viral Spread ◽  
Highly Efficient ◽  
Systemic Spread

ABSTRACTNipah virus (NiV) and Hendra virus (HeV) are closely related henipaviruses of theParamyxovirinae. Spillover from their fruit bat reservoirs can cause severe disease in humans and livestock. Despite their high sequence similarity, NiV and HeV exhibit apparent differences in receptor and tissue tropism, envelope-mediated fusogenicity, replicative fitness, and other pathophysiologic manifestations. To investigate the molecular basis for these differences, we first established a highly efficient reverse genetics system that increased rescue titers by ≥3 log units, which offset the difficulty of generating multiple recombinants under constraining biosafety level 4 (BSL-4) conditions. We then replaced, singly and in combination, the matrix (M), fusion (F), and attachment glycoprotein (G) genes in mCherry-expressing recombinant NiV (rNiV) with their HeV counterparts. These chimeric but isogenic rNiVs replicated well in primary human endothelial and neuronal cells, indicating efficient heterotypic complementation. The determinants of budding efficiency, fusogenicity, and replicative fitness were dissociable: HeV-M budded more efficiently than NiV-M, accounting for the higher replicative titers of HeV-M-bearing chimeras at early times, while the enhanced fusogenicity of NiV-G-bearing chimeras did not correlate with increased replicative fitness. Furthermore, to facilitate spatiotemporal studies on henipavirus pathogenesis, we generated a firefly luciferase-expressing NiV and monitored virus replication and spread in infected interferon alpha/beta receptor knockout mice via bioluminescence imaging. While intraperitoneal inoculation resulted in neuroinvasion following systemic spread and replication in the respiratory tract, intranasal inoculation resulted in confined spread to regions corresponding to olfactory bulbs and salivary glands before subsequent neuroinvasion. This optimized henipavirus reverse genetics system will facilitate future investigations into the growing numbers of novel henipavirus-like viruses.IMPORTANCENipah virus (NiV) and Hendra virus (HeV) are recently emergent zoonotic and highly lethal pathogens with pandemic potential. Although differences have been observed between NiV and HeV replication and pathogenesis, the molecular basis for these differences has not been examined. In this study, we established a highly efficient system to reverse engineer changes into replication-competent NiV and HeV, which facilitated the generation of reporter-expressing viruses and recombinant NiV-HeV chimeras with substitutions in the genes responsible for viral exit (the M gene, critical for assembly and budding) and viral entry (the G [attachment] and F [fusion] genes). These chimeras revealed differences in the budding and fusogenic properties of the M and G proteins, respectively, which help explain previously observed differences between NiV and HeV. Finally, to facilitate futurein vivostudies, we monitored the replication and spread of a bioluminescent reporter-expressing NiV in susceptible mice; this is the first time suchin vivoimaging has been performed under BSL-4 conditions.

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