scholarly journals Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array

2021 ◽  
Vol 7 (22) ◽  
pp. eabe7738
Author(s):  
Qiang Zheng ◽  
Mingzeng Peng ◽  
Zhuo Liu ◽  
Shuyu Li ◽  
Rongcheng Han ◽  
...  

Dynamic mapping of the cell-generated force of cardiomyocytes will help provide an intrinsic understanding of the heart. However, a real-time, dynamic, and high-resolution mapping of the force distribution across a single living cell remains a challenge. Here, we established a force mapping method based on a “light nano-antenna” array with the use of piezo-phototronic effect. A spatial resolution of 800 nm and a temporal resolution of 333 ms have been demonstrated for force mapping. The dynamic mapping of cell force of live cardiomyocytes was directly derived by locating the antennas’ positions and quantifying the light intensities of the piezo-phototronic light nano-antenna array. This study presents a rapid and ultrahigh-resolution methodology for the fundamental study of cardiomyocyte behavior at the cell or subcellular level. It can provide valuable information about disease detection, drug screening, and tissue engineering for heart-related studies.

EP Europace ◽  
2021 ◽  
Vol 23 (Supplement_3) ◽  
Author(s):  
A Di Cori ◽  
M Rillo ◽  
A Sultan ◽  
H Ramanna ◽  
I Deisenhofer ◽  
...  

Abstract Funding Acknowledgements Type of funding sources: None. Introduction Previous publications suggest that the use of high-density (HD) mapping leads to better substrate visualization and may lead to improved procedural outcomes. A novel dynamic mapping software, utilizes the HD grid mapping catheter (HD Grid) to display beat-to-beat, dynamic regional mapping data (LiveView). Incorporation of real-time dynamic mapping data into routine mapping/ablation workflows may further enhance the clinical benefits of HD mapping during radiofrequency (RF) catheter ablation procedures. Purpose  To examine the clinical utility and common workflows when dynamic mapping data was used during RF ablation procedures among operators with various experience levels. Methods Observational procedural data including procedure time, total RF time, and workflow preference were prospectively collected in catheter ablation cases utilizing LiveView from May to September 2020. Mapping and ablation strategies were determined at the operator’s discretion. Total percentage exceed 100% when multiple usage were reported. Results A total of 428 cases were collected from over 25 operators in 11 European countries. LiveView was used in a variety of cases including atrial fibrillation (paroxysmal and persistent), atrial flutter (typical and atypical), and VT (ischemic, non-ischemic, and idiopathic). Visualization of real-time mapping data from the current location of the HD Grid was commonly used after creation of traditional full-chamber maps (319/428, 74.5%). While operators in over 55% of the cases indicated that the use of dynamic display during mapping helped identify areas that were under ablated (238/428, 55.6%), using LiveView did not affect the lesion delivery strategies in those regions. LiveView was also used as a primary method for confirmation of pulmonary vein isolation (PVI) in 213 cases (49.8%). The most common reported usage of LiveView among the 428 cases analyzed was PVI confirmation/gap identification (75.2%), ablation line gap identification (41.1)%, and identification of breakthrough activation (23.6%) Conclusions This initial analysis demonstrated the diverse clinical utilization of LiveView dynamic display during RF catheter ablation procedures, including atrial and ventricular arrhythmias. Without causing significant changes to normal workflow, dynamic display of regional signals allows for rapid identification of ablation targets. When used during RF delivery, real-time assessment of regional activation patterns helped improve outcomes by rapidly identifying critical ablation location and ensuring successful lesion delivery. A further study that examines the impact of dynamic display on procedure efficacy may be warranted.


2016 ◽  
Vol 4 (4) ◽  
pp. 649-655 ◽  
Author(s):  
Yusuke Kambe ◽  
Katsura Kojima ◽  
Naohide Tomita ◽  
Yasushi Tamada ◽  
Tetsuji Yamaoka

A recombinant tension sensor immobilized to a surface enables the real-time visualization of cell traction force at the focal adhesion level.


CICTP 2020 ◽  
2020 ◽  
Author(s):  
Lina Mao ◽  
Wenquan Li ◽  
Pengsen Hu ◽  
Guiliang Zhou ◽  
Huiting Zhang ◽  
...  

2021 ◽  
Vol 11 (4) ◽  
pp. 1933
Author(s):  
Hiroomi Hikawa ◽  
Yuta Ichikawa ◽  
Hidetaka Ito ◽  
Yutaka Maeda

In this paper, a real-time dynamic hand gesture recognition system with gesture spotting function is proposed. In the proposed system, input video frames are converted to feature vectors, and they are used to form a posture sequence vector that represents the input gesture. Then, gesture identification and gesture spotting are carried out in the self-organizing map (SOM)-Hebb classifier. The gesture spotting function detects the end of the gesture by using the vector distance between the posture sequence vector and the winner neuron’s weight vector. The proposed gesture recognition method was tested by simulation and real-time gesture recognition experiment. Results revealed that the system could recognize nine types of gesture with an accuracy of 96.6%, and it successfully outputted the recognition result at the end of gesture using the spotting result.


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