scholarly journals Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA

2021 ◽  
Vol 7 (42) ◽  
Author(s):  
Itxaso Anso ◽  
Luis G. M. Basso ◽  
Lei Wang ◽  
Alberto Marina ◽  
Edgar D. Páez-Pérez ◽  
...  

2010 ◽  
Vol 75 (5) ◽  
pp. 1272-1284 ◽  
Author(s):  
Marc Erhardt ◽  
Takanori Hirano ◽  
Yichu Su ◽  
Koushik Paul ◽  
Daniel H. Wee ◽  
...  
Keyword(s):  


Biochemistry ◽  
1991 ◽  
Vol 30 (29) ◽  
pp. 7283-7297 ◽  
Author(s):  
Otto G. Berg ◽  
Bao Zhu Yu ◽  
Joe Rogers ◽  
Mahendra Kumar Jain


2021 ◽  
Author(s):  
Shenghua Ma ◽  
Wei Zong ◽  
Xiaojun Han

Schematic diagram of interfacial catalysis reactions by MRGO–Pd Pickering emulsion.



2021 ◽  
pp. 138441
Author(s):  
Alexander G. Volkov ◽  
Asya Bookal ◽  
Jewel S. Hairston ◽  
Jade Roberts ◽  
Gamaliel Taengwa ◽  
...  


2020 ◽  
Vol 49 (19) ◽  
pp. 6183-6190 ◽  
Author(s):  
Ganguri Sarada ◽  
Ahreum Kim ◽  
Dongwon Kim ◽  
Ok-Sang Jung

This pliable double cage is an efficient, tolerant, and reproducible porous matrix for diverse anion exchange, which is as a ruler for recognition of ubiquitous polyatomic anions.



2012 ◽  
Vol 194 (18) ◽  
pp. 4797-4797 ◽  
Author(s):  
Shin-Ichi Aizawa
Keyword(s):  


2010 ◽  
Vol 46 (6) ◽  
pp. 949-951 ◽  
Author(s):  
Hongwei Ma ◽  
Jian’an He ◽  
Zhiqiang Zhu ◽  
Bei’er Lv ◽  
Di Li ◽  
...  


2007 ◽  
Vol 282 (28) ◽  
pp. 20705-20714 ◽  
Author(s):  
Marcelo E. Guerin ◽  
Jana Kordulakova ◽  
Francis Schaeffer ◽  
Zuzana Svetlikova ◽  
Alejandro Buschiazzo ◽  
...  


2000 ◽  
Vol 113 (8) ◽  
pp. 1405-1414 ◽  
Author(s):  
P.F. van der Ven ◽  
J.W. Bartsch ◽  
M. Gautel ◽  
H. Jockusch ◽  
D.O. Furst

Titin, also called connectin, is a giant muscle protein that spans the distance from the sarcomeric Z-disc to the M-band. Titin is thought to direct the assembly of sarcomeres and to maintain sarcomeric integrity by interacting with numerous sarcomeric proteins and providing a mechanical linkage. Since severe defects of such an important molecule are likely to result in embryonic lethality, a cell culture model should offer the best practicable tool to probe the cellular functions of titin. The myofibroblast cell line BHK-21/C13 was described to assemble myofibrils in culture. We have now characterized the sub-line BHK-21-Bi, which bears a small deletion within the titin gene. RNA analysis revealed that in this mutant cell line only a small internal portion of the titin mRNA is deleted. However, western blots, immunofluorescence microscopy and immunoprecipitation experiments showed that only the N-terminal, approx. 100 kDa central Z-disc portion of the 3 MDa titin protein is expressed, due to the homozygous deletion in the gene. Most importantly, in BHK-21-Bi cells the formation of thick myosin filaments and the assembly of myofibrils are impaired, although sarcomeric proteins are expressed. Lack of thick filament formation and of ordered actin-myosin arrays was confirmed by electron microscopy. Myogenisation induced by transfection with MyoD yielded myofibrils only in myotubes formed from wild type and not from mutant cells, ruling out that a principal failure in myogenic commitment of the BHK-21-Bi cells might cause the observed effects. These experiments provide the first direct evidence for the crucial role of titin in both thick filament formation as a molecular ruler and in the coordination of myofibrillogenesis.



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