Targeted gene replacement in Drosophila via P element-induced gap repair

Science ◽  
1991 ◽  
Vol 253 (5024) ◽  
pp. 1110-1117 ◽  
Author(s):  
G. Gloor ◽  
N. Nassif ◽  
D. Johnson-Schlitz ◽  
C. Preston ◽  
W. Engels
Keyword(s):  
Gene Replacement ◽  
P Element ◽  
Targeted Gene Replacement

scholarly journals Elimination of Introns at the Drosophila suppressor-of-forked Locus by P-Element-Mediated Gene Conversion Shows That an RNA Lacking a Stop Codon is Dispensable

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 345-351
Author(s):  
Carol J Williams ◽  
Kevin O'Hare
Keyword(s):  
X Chromosome ◽  
Stop Codon ◽  
Alternative Polyadenylation ◽  
Gene Replacement ◽  
P Element ◽  
Wild Type ◽  
Genomic Location ◽  
Chromosomal Breaks ◽  
White Locus ◽  
Cleavage Stimulation Factor

Abstract The suppressor of forked [su(f)] locus affects the phenotype of mutations caused by transposable element insertions at unlinked loci. It encodes a putative 84-kD protein with homology to two proteins involved in mRNA 3′ end processing; the product of the yeast RNA14 gene and the 77-kD subunit of human cleavage stimulation factor. Three su(f) mRNAs are produced by alternative polyadenylation. The 2. 6 and 2.9-kb mRNAs encode the same 84-kD protein while a 1.3-kb RNA, which terminates within the fourth intron, is unusual in having no stop codon. Using P-element-mediated gene replacement we have copied sequences from a transformation construct into the su(f) gene creating a su(f) allele at the normal genomic location that lacks the first five introns. This allele is viable and appears wild type for su(f) function, demonstrating that the 1.3-kb RNA and the sequences contained within the deleted introns are dispensable for su(f) function. Compared with studies on gene replacement at the white locus, chromosomal breaks at su(f) appear to be less efficiently repaired from ectopic sites, perhaps because of the location of su(f) at the euchromatin/heterochromatin boundary on the X chromosome.

scholarly journals A Homeobox Gene Is Essential for Conidiogenesis of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (4) ◽  
pp. 366-375 ◽  
Author(s):  
Wende Liu ◽  
Shiyong Xie ◽  
Xinhua Zhao ◽  
Xin Chen ◽  
Wenhui Zheng ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Homeobox Gene ◽  
Gene Replacement ◽  
Fungal Genome ◽  
Protein Signaling ◽  
Homeodomain Proteins ◽  
Conidial Production ◽  
Targeted Gene Replacement ◽  
Severity Of The Disease

Magnaporthe oryzae starts its infection by the attachment of pyriform conidia on rice tissues, and severity of the disease epidemic is proportional to the quantity of conidia produced in the rice blast lesions. However, the mechanism of conidial production is not well understood. Homeodomain proteins play critical roles in regulating various growth and developmental processes in fungi and other eukaryotes. Through targeted gene replacement, we find that deletion of HTF1, one of seven homeobox genes in the fungal genome, does not affect mycelial growth but causes total defect of conidial production. Further observation revealed that the Δhtf1 mutant produces significantly more conidiophores, which curve slightly near the tip but could not develop sterigmata-like structures. Although the Δhtf1 mutant fails to form conidia, it could still develop melanized appressoria from hyphal tips and infect plants. The expression level of HTF1 is significantly reduced in the Δmgb1 G-β and ΔcpkA deletion mutant, and the ACR1 but not CON7 gene that encodes transcription factor required for normal conidiogenesis is significantly downregulated in the Δhtf1 mutant. These data suggest that the HTF1 gene is essential for conidiogenesis, and may be functionally related to the trimeric G-protein signaling and other transcriptional regulators that are known to be important for conidiation in M. oryzae.

scholarly journals Mice with defects in HB-EGF ectodomain shedding show severe developmental abnormalities

2003 ◽  
Vol 163 (3) ◽  
pp. 469-475 ◽  
Author(s):  
Satoru Yamazaki ◽  
Ryo Iwamoto ◽  
Kazuko Saeki ◽  
Masanori Asakura ◽  
Seiji Takashima ◽  
...  
Keyword(s):  
Heart Valves ◽  
Transmembrane Domain ◽  
Severe Heart Failure ◽  
Gene Replacement ◽  
Soluble Form ◽  
Ectodomain Shedding ◽  
Heparin Binding ◽  
Developmental Abnormalities ◽  
Targeted Gene Replacement

Heparin-binding EGF-like growth factor (HB-EGF) is first synthesized as a membrane-anchored form (proHB-EGF), and its soluble form (sHB-EGF) is released by ectodomain shedding from proHB-EGF. To examine the significance of proHB-EGF processing in vivo, we generated mutant mice by targeted gene replacement, expressing either an uncleavable form (HBuc) or a transmembrane domain–truncated form (HBΔtm) of the molecule. HBuc/uc mice developed severe heart failure and enlarged heart valves, phenotypes similar to those in proHB-EGF null mice. On the other hand, mice carrying HBΔtm exhibited severe hyperplasia in both skin and heart. These results indicate that ectodomain shedding of proHB-EGF is essential for HB-EGF function in vivo, and that this process requires strict control.

scholarly journals Apolipoprotein E genotype affects tissue metallothionein levels: studies in targeted gene replacement mice

2012 ◽  
Vol 7 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Anne-Christin Graeser ◽  
Patricia Huebbe ◽  
Niels Storm ◽  
Wolfgang Höppner ◽  
Frank Döring ◽  
...  
Keyword(s):  
Apolipoprotein E ◽  
Gene Replacement ◽  
Targeted Gene Replacement ◽  
Apolipoprotein E Genotype

Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus

2015 ◽  
Vol 112 (12) ◽  
pp. 2543-2549 ◽  
Author(s):  
Takayuki Arazoe ◽  
Kennosuke Miyoshi ◽  
Tohru Yamato ◽  
Tetsuo Ogawa ◽  
Shuichi Ohsato ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Highly Efficient ◽  
Blast Fungus ◽  
Targeted Gene Replacement

scholarly journals Double targeted gene replacement for creating null mutants.

1991 ◽  
Vol 88 (16) ◽  
pp. 7170-7174 ◽  
Author(s):  
A. Cruz ◽  
C. M. Coburn ◽  
S. M. Beverley
Keyword(s):  
Gene Replacement ◽  
Targeted Gene Replacement ◽  
Null Mutants
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