scholarly journals Antimalarial Activity of Tigecycline, a Novel Glycylcycline Antibiotic

2009 ◽  
Vol 53 (9) ◽  
pp. 4040-4042 ◽  
Author(s):  
P. Starzengruber ◽  
K. Thriemer ◽  
R. Haque ◽  
W. A. Khan ◽  
H. P. Fuehrer ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Confidence Interval ◽  
Antimalarial Activity ◽  
Drug Susceptibility ◽  
Clinical Isolates ◽  
In Vitro Drug Susceptibility ◽  
Antibacterial Spectrum ◽  
Susceptibility Assay ◽  
Broad Antibacterial Spectrum

ABSTRACT Tigecycline is a novel glycylcycline antibiotic with a broad antibacterial spectrum. Tigecycline was tested with 66 clinical isolates of Plasmodium falciparum from Bangladesh using the histidine-rich protein 2 in vitro drug susceptibility assay. The 50% and 90% inhibitory concentrations of tigecycline were 699 (95% confidence interval, 496 to 986) and 5,905 nM (4,344 to 8,028). Tigecycline shows no activity correlation with traditional antimalarials and has substantial antimalarial activity on its own.

Growth, Drug Susceptibility, and Gene Expression Profiling of Plasmodium falciparum Cultured in Medium Supplemented with Human Serum

2007 ◽  
Vol 12 (8) ◽  
pp. 1109-1114 ◽  
Author(s):  
Kshipra Singh ◽  
Ameeta Agarwal ◽  
Shabana I. Khan ◽  
Larry A. Walker ◽  
Babu L. Tekwani
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Human Serum ◽  
Global Gene Expression ◽  
Drug Susceptibility ◽  
Growth Cycle ◽  
In Vitro Cultivation ◽  
Human Malaria Parasite ◽  
In Vitro Drug Susceptibility

In vitro cultivation of Plasmodium falciparum has been extremely useful in understanding the biology of the human malaria parasite as well as research on the discovery of new antimalarial drugs and vaccines. A chemically defined serum-free medium supplemented with lipid-rich bovine serum albumin (AlbuMAX I) offers the following advantages over human serum-supplemented media for the in vitro culture of P. falciparum: 1) improved growth profile, with more than a 2-fold higher yield of the parasites at any stage of the growth cycle; 2) suitability for in vitro antimalarial screening, as the parasites grown in AlbuMAX and human serum-supplemented media show similar sensitivity to standard and novel antimalarials as well as natural product extracts in the in vitro drug susceptibility assays; and 3) DNA microarray analysis comparing the global gene expression profile of sorbitol-synchronized P. falciparum trophozoites grown in the 2 different media, indicating minimal differences. ( Journal of Biomolecular Screening 2007:1109-1114)

scholarly journals Optimizing the HRP-2 in vitro malaria drug susceptibility assay using a reference clone to improve comparisons of Plasmodium falciparum field isolates

2012 ◽  
Vol 11 (1) ◽  
pp. 325 ◽  
Author(s):  
Wiriya Rutvisuttinunt ◽  
Suwanna Chaorattanakawee ◽  
Stuart D Tyner ◽  
Paktiya Teja-isavadharm ◽  
Youry Se ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Susceptibility ◽  
Field Isolates ◽  
Susceptibility Assay

scholarly journals Nonlinear Mixed-Effects Modelling of In Vitro Drug Susceptibility and Molecular Correlates of Multidrug Resistant Plasmodium falciparum

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69505 ◽  
Author(s):  
Julie A. Simpson ◽  
Kris M. Jamsen ◽  
Tim J. C. Anderson ◽  
Sophie Zaloumis ◽  
Shalini Nair ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Mixed Effects ◽  
Nonlinear Mixed Effects ◽  
In Vitro Drug Susceptibility

scholarly journals Plasmodium falciparum-Based Bioassay for Measurement of Artemisinin Derivatives in Plasma or Serum

2004 ◽  
Vol 48 (3) ◽  
pp. 954-960 ◽  
Author(s):  
Paktiya Teja-Isavadharm ◽  
James O. Peggins ◽  
Thomas G. Brewer ◽  
Nicholas J. White ◽  
H. Kyle Webster ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
High Performance ◽  
Antimalarial Activity ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Response Curves ◽  
Artemisinin Derivatives ◽  
Treatment Regimens ◽  
Limit Of Quantitation

ABSTRACT Artemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily available and reproducible assays to monitor drug levels closely in patients. A sensitive and reproducible bioassay for the measurement of the concentrations of artemisinin derivatives in plasma and serum is described. By modifying the in vitro drug susceptibility test, it was found that antimalarial activity in plasma or serum containing an unknown concentration of drug could be equated to the known concentrations of dihydroartemisinin (DHA) required to inhibit parasite growth. Dose-response curves for a Plasmodium falciparum clone (clone W2) and DHA were used as a standard for each assay. Assays with plasma or serum spiked with DHA proved to be reproducible (coefficient of variation, ≤10.9%), with a lower limit of quantitation equivalent to 2.5 ng of DHA per ml. For plasma spiked with artesunate or artemether, there was good agreement of the results obtained by the bioassay and the concentrations measured by high-performance liquid chromatography (HPLC) with electrochemical detection. The bioassay for measurement of the antimalarial activities of artemisinin derivatives in body fluids requires a smaller volume of plasma or serum and is more sensitive than the presently available HPLC methods, can provide pharmacodynamic parameters for determination of activity against the parasite, and should enhance the design of more appropriate dosage regimens for artemisinin drugs.

scholarly journals Drug Susceptibility and Genetic Evaluation of Plasmodium falciparum Isolates Obtained in Four Distinct Geographical Regions of Kenya

2004 ◽  
Vol 48 (9) ◽  
pp. 3598-3601 ◽  
Author(s):  
Abigael Mbaisi ◽  
Pamela Liyala ◽  
Fredrick Eyase ◽  
Rachel Achilla ◽  
Hosea Akala ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Drug Susceptibility ◽  
Antimalarial Drugs ◽  
Genetic Evaluation ◽  
Geographical Regions ◽  
In Vitro Drug Susceptibility

ABSTRACT The drug resistance profiles of Plasmodium falciparum isolated from four regions in Kenya were analyzed for drug resistance profiles. We observed variability in resistance to a broad range of antimalarial drugs across Kenya as determined from in vitro drug susceptibility screening and genotyping analysis.

scholarly journals In vitro evaluation of erythromycin in chloroquine resistant brazilian P. falciparum freshly isolates: modulating effect and antimalarial activity evidence

1999 ◽  
Vol 41 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Carla M. S. MENEZES ◽  
Karin KIRCHGATTER ◽  
Sílvia M. F. DI SANTI ◽  
Carine SAVALLI ◽  
Fabíola G. MONTEIRO ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antimicrobial Therapy ◽  
Antimalarial Activity ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Chloroquine Resistance ◽  
Reversal Agent ◽  
Resistance Modulation ◽  
Erythromycin A

Erythromycin, a reversal agent in multidrug-resistant cancer, was assayed in chloroquine resistance modulation. The in vitro microtechnique for drug susceptibility was employed using two freshly isolates of Plasmodium falciparum from North of Brazil. The antimalarial effect of the drug was confirmed, with an IC50 estimates near the usual antimicrobial therapy concentration, and a significant statistical modulating action was observed for one isolate.

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