First Isolation of blaVIM-2 in an Environmental Isolate of Pseudomonas pseudoalcaligenes

ABSTRACT Biphenyl dioxygenase (Bph Dox) catalyzes the initial oxygenation of biphenyl and related compounds. Bph Dox is a multicomponent enzyme in which a large subunit (encoded by the bphA1 gene) is significantly responsible for substrate specificity. By using the process of DNA shuffling of bphA1 of Pseudomonas pseudoalcaligenes KF707 and Burkholderia cepaciaLB400, a number of evolved Bph Dox enzymes were created. Among them, anEscherichia coli clone expressing chimeric Bph Dox exhibited extremely enhanced benzene-, toluene-, and alkylbenzene-degrading abilities. In this evolved BphA1, four amino acids (H255Q, V258I, G268A, and F277Y) were changed from the KF707 enzyme to those of the LB400 enzyme. Subsequent site-directed mutagenesis allowed us to determine the amino acids responsible for the degradation of monocyclic aromatic hydrocarbons.

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Draft Genome Sequence of the Plant Growth-Promoting Bacterium Pseudomonas pseudoalcaligenes KB-10

Microbiology Resource Announcements ◽

10.1128/mra.00311-21 ◽

2021 ◽

Vol 10 (20) ◽

Author(s):

M. M. A. Khan ◽

Jin Duan ◽

Bernard R. Glick ◽

Patrick M. Finnegan ◽

Saleh A. Kabli ◽

...

Keyword(s):

Plant Growth ◽

Genome Sequence ◽

Draft Genome ◽

Gc Content ◽

Draft Genome Sequence ◽

Circular Chromosome ◽

Pseudomonas Pseudoalcaligenes ◽

Content Type ◽

Plant Growth Promoting ◽

Growth Promoting

ABSTRACT Pseudomonas pseudoalcaligenes KB-10 can enhance salinity tolerance in coriander plants. We report a draft genome sequence of P. pseudoalcaligenes KB-10, comprising a 5,241,174-bp circular chromosome containing 4,921 genes, with a GC content of 62.97%.

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The membrane-bound respiratory chain of Pseudomonas pseudoalcaligenes KF707 cells grown in the presence or absence of potassium tellurite This work is dedicated to my friend and colleague Franco Tatò, prematurely deceased on 7 July 2001.

Microbiology ◽

10.1099/00221287-148-6-1699 ◽

2002 ◽

Vol 148 (6) ◽

pp. 1699-1708 ◽

Cited By ~ 38

Author(s):

Giovanna Di Tomaso ◽

Stefano Fedi ◽

Monica Carnevali ◽

Marco Manegatti ◽

Carlo Taddei ◽

...

Keyword(s):

Respiratory Chain ◽

Pseudomonas Pseudoalcaligenes ◽

Potassium Tellurite ◽

Membrane Bound

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PredicTF: a tool to predict bacterial transcription factors in complex microbial communities

10.1101/2021.01.28.428666 ◽

2021 ◽

Author(s):

Lummy Maria Oliveira Monteiro ◽

Joao Saraiva ◽

Rodolfo Brizola Toscan ◽

Peter F Stadler ◽

Rafael Silva-Rocha ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Microbial Communities ◽

Open Source ◽

Genetic Information ◽

Environmental Isolate ◽

Ammonium Oxidation ◽

Cellular Gene ◽

Bacterial Transcription

AbstractTranscription Factors (TFs) are proteins that control the flow of genetic information by regulating cellular gene expression. Here we describe PredicTF, a first platform supporting the prediction and classification of novel bacterial TF in complex microbial communities. We evaluated PredicTF using a two-step approach. First, we tested PredictTF’s ability to predict TFs for the genome of an environmental isolate. In the second evaluation step, PredicTF was used to predict TFs in a metagenome and 11 metatranscriptomes recovered from a community performing anaerobic ammonium oxidation (anammox) in a bioreactor. PredicTF is open source pipeline available at https://github.com/mdsufz/PredicTF.

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