scholarly journals Expression of Reductive Dehalogenase Genes in Dehalococcoides ethenogenes Strain 195 Growing on Tetrachloroethene, Trichloroethene, or 2,3-Dichlorophenol

2007 ◽  
Vol 73 (14) ◽  
pp. 4439-4445 ◽  
Author(s):  
Jennifer M. Fung ◽  
Robert M. Morris ◽  
Lorenz Adrian ◽  
Stephen H. Zinder
Keyword(s):  
Reductive Dechlorination ◽  
Stop Codon ◽  
Gene Transcript ◽  
High Coverage ◽  
Transcript Levels ◽  
Reductive Dehalogenase ◽  
Dehalococcoides Ethenogenes ◽  
Rd Cells ◽  
Reductive Dehalogenase Genes ◽  
In Cells

ABSTRACT Reductive dehalogenase (RD) gene transcript levels in Dehalococcoides ethenogenes strain 195 were investigated using reverse transcriptase quantitative PCR during growth and reductive dechlorination of tetrachloroethene (PCE), trichloroethene (TCE), or 2,3-dichlorophenol (2,3-DCP). Cells grown with PCE or TCE had high transcript levels (greater than that for rpoB) for tceA, which encodes the TCE RD, pceA, which encodes the PCE RD, and DET0162, which contains a predicted stop codon and is considered nonfunctional. In cells grown with 2,3-DCP, tceA mRNA was less than 1% of that for rpoB, indicating that its transcription was regulated. pceA and DET0162 were the only RD genes with high transcript levels in cells grown with 2,3-DCP. Proteomic analysis of PCE-grown cells detected both PceA and TceA with high peptide coverage but not DET0162, and analysis of 2,3-DCP-grown cells detected PceA with high coverage but not TceA, DET0162, or any other potential RD. Cells grown with PCE or 2,3-DCP were tested for the ability to dechlorinate PCE, TCE, or 2,3-DCP with H2 as the electron donor. 2,3-DCP-grown cells were unable to dechlorinate TCE but dechlorinated PCE to TCE without a lag, and PCE-grown cells dechlorinated 2,3-DCP without a lag. These results show that 2,3-DCP-grown cells do not produce TceA and that DET0162 is transcribed but its translation product is not detectable in cells and are consistent with PceA's being bifunctional, also serving as the 2,3-DCP RD. Chlorophenols naturally occur in soils and are good candidates for the original substrates for PceA.

scholarly journals Characterization of Hydrogenase and Reductive Dehalogenase Activities of Dehalococcoides ethenogenes Strain 195

2005 ◽  
Vol 71 (3) ◽  
pp. 1664-1667 ◽  
Author(s):  
Ivonne Nijenhuis ◽  
Stephen H. Zinder
Keyword(s):  
Reductive Dechlorination ◽  
Methyl Viologen ◽  
Hydrogenase Activity ◽  
Atpase Inhibitor ◽  
Benzyl Viologen ◽  
Reductive Dehalogenase ◽  
Cell Extracts ◽  
Dehalococcoides Ethenogenes ◽  
In Cells

ABSTRACT Dehalococcoides ethenogenes strain 195 reductively dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to vinyl chloride and ethene using H2 as an electron donor. PCE- and TCE-reductive dehalogenase (RD) activities were mainly membrane associated, whereas only about 20% of the hydrogenase activity was membrane associated. Experiments with methyl viologen (MV) were consistent with a periplasmic location for the RDs or a component feeding electrons to them. The protonophore uncoupler tetrachlorosalicylanilide did not inhibit reductive dechlorination in cells incubated with H2 and PCE and partially restored activity in cells incubated with the ATPase inhibitor N,N′-dicyclohexylcarbodiimide. Benzyl viologen or diquat (E o′ ≈ −360 mV) supported reductive dechlorination of PCE or TCE at rates comparable to MV (−450 mV) in cell extracts.

scholarly journals Complete Genome Sequence of Dehalococcoides mccartyi Strain FL2, a Trichloroethene-Respiring Anaerobe Isolated from Pristine Freshwater Sediment

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Jun Yan ◽  
Yi Yang ◽  
Xiuying Li ◽  
Frank E. Löffler
Keyword(s):  
Complete Genome Sequence ◽  
Reductive Dechlorination ◽  
Complete Genome ◽  
Hydrogen Oxidation ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Reductive Dehalogenase ◽  
Dehalococcoides Mccartyi ◽  
Reductive Dehalogenase Genes

Dehalococcoides mccartyi strain FL2 couples growth to hydrogen oxidation and reductive dechlorination of trichloroethene and cis- and trans-1,2-dichloroethenes. Strain FL2 has a 1.42-Mb genome with a G+C content of 47.0% and carries 1,465 protein-coding sequences, including 24 reductive dehalogenase genes.

scholarly journals Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols

2011 ◽  
Vol 77 (17) ◽  
pp. 6261-6264 ◽  
Author(s):  
Ariane Bisaillon ◽  
Réjean Beaudet ◽  
François Lépine ◽  
Richard Villemur
Keyword(s):  
Quantitative Analysis ◽  
Transcript Levels ◽  
Content Type ◽  
Reductive Dehalogenase ◽  
Reductive Dehalogenase Genes ◽  
Desulfitobacterium Hafniense

ABSTRACTRelative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2,cprA3,cprA4, andcprA5) inDesulfitobacterium hafniensePCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures,cprA2andcprA3were upregulated, as wascprA5, but concomitantly with the appearance of 2,4-dichlorophenol (DCP). In 3,5-DCP-amended cultures, onlycprA5was upregulated. In pentachlorophenol-amended cultures grown for 12 h,cprA2andcprA3were upregulated but notcprA5. cprA4was not upregulated significantly in cultures containing any tested chlorophenols.

scholarly journals Indications for Acquisition of Reductive Dehalogenase Genes through Horizontal Gene Transfer by Dehalococcoides ethenogenes Strain 195

2005 ◽  
Vol 71 (6) ◽  
pp. 2955-2961 ◽  
Author(s):  
Christophe Regeard ◽  
Julien Maillard ◽  
Christine Dufraigne ◽  
Patrick Deschavanne ◽  
Christof Holliger
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Gc Content ◽  
Hierarchical Classification ◽  
Reductive Dehalogenase ◽  
Dehalococcoides Ethenogenes ◽  
Bioinformatic Tool ◽  
Key Enzyme ◽  
Reductive Dehalogenase Genes

ABSTRACT The genome of Dehalococcoides ethenogenes strain 195, an anaerobic dehalorespiring bacterium, contains 18 copies of putative reductive dehalogenase genes, including the well-characterized tceA gene, whose gene product functions as the key enzyme in the environmentally important dehalorespiration process. The genome of D. ethenogenes was analyzed using a bioinformatic tool based on the frequency of oligonucleotides. The results in the form of a genomic signature revealed several local disruptions of the host signature along the genome sequence. These fractures represent DNA segments of potentially foreign origin, so-called atypical regions, which may have been acquired by an ancestor through horizontal gene transfer. Most interestingly, 15 of the 18 reductive dehalogenase genes, including the tceA gene, were found to be located in these regions, strongly indicating the foreign nature of the dehalorespiration activity. The GC content and the presence of recombinase genes within some of these regions corroborate this hypothesis. A hierarchical classification of the atypical regions containing the reductive dehalogenase genes indicated that these regions were probably acquired by several gene transfer events.

Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by “Dehalococcoides ethenogenes” 195

1999 ◽  
Vol 65 (7) ◽  
pp. 3108-3113 ◽  
Author(s):  
Xavier Maymó-Gatell ◽  
Timothy Anguish ◽  
Stephen H. Zinder
Keyword(s):  
Vinyl Chloride ◽  
Growth Medium ◽  
Electron Acceptor ◽  
Reductive Dechlorination ◽  
Electron Acceptors ◽  
Chlorinated Compounds ◽  
Reductive Dehalogenase ◽  
Dehalococcoides Ethenogenes ◽  
Over Time ◽  
Number Of Cells

ABSTRACT “Dehalococcoides ethenogenes” 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol/vol] inoculum) into growth medium amended with trichloroethene (TCE),cis-dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron acceptor, these chlorinated compounds were consumed at increasing rates over time, which indicated that growth occurred. Moreover, the number of cells increased when TCE, 1,1-DCE, or DCA was present. PCE, TCE, 1,1-DCE, and cis-DCE were converted mainly to vinyl chloride (VC) and then to ETH, while DCA was converted to ca. 99% ETH and 1% VC. cis-DCE was used at lower rates than PCE, TCE, 1,1-DCE, or DCA was used. When PCE-grown cultures were transferred to media containing VC ortrans-DCE, products accumulated slowly, and there was no increase in the rate, which indicated that these two compounds did not support growth. When the intermediates in PCE dechlorination by strain 195 were monitored, TCE was detected first, followed bycis-DCE. After a lag, VC, 1,1-DCE, andtrans-DCE accumulated, which is consistent with the hypothesis that cis-DCE is the precursor of these compounds. Both cis-DCE and 1,1-DCE were eventually consumed, and both of these compounds could be considered intermediates in PCE dechlorination, whereas the small amount oftrans-DCE that was produced persisted. Cultures grown on TCE, 1,1-DCE, or DCA could immediately dechlorinate PCE, which indicated that PCE reductive dehalogenase activity was constitutive when these electron acceptors were used.

scholarly journals Complete Genome Sequence of Sulfurospirillum sp. Strain ACSDCE, an Anaerobic Bacterium That Respires Tetrachloroethene under Acidic pH Conditions

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Yi Yang ◽  
Leitao Huo ◽  
Xiuying Li ◽  
Jun Yan ◽  
Frank E. Löffler
Keyword(s):  
Genome Sequence ◽  
Reductive Dechlorination ◽  
Anaerobic Bacterium ◽  
Acidic Ph ◽  
Protein Coding ◽  
Coding Sequences ◽  
Reductive Dehalogenase ◽  
Ph Values ◽  
Ph Conditions ◽  
Reductive Dehalogenase Genes

ABSTRACT Sulfurospirillum sp. strain ACSDCE couples growth with reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene at pH values as low as 5.5. The genome sequence of strain ACSDCE consists of a circular 2,737,849-bp chromosome and a 39,868-bp plasmid and carries 2,737 protein-coding sequences, including two reductive dehalogenase genes.

scholarly journals Genome Sequence of “Candidatus Dehalogenimonas etheniformans” Strain GP, a Vinyl Chloride-Respiring Anaerobe

2020 ◽  
Vol 9 (50) ◽  
Author(s):  
Yi Yang ◽  
Jun Yan ◽  
Xiuying Li ◽  
Yan Lv ◽  
Yiru Cui ◽  
...  
Keyword(s):  
Vinyl Chloride ◽  
Genome Sequence ◽  
Reductive Dechlorination ◽  
Reductive Dehalogenase ◽  
Reductive Dehalogenase Genes

ABSTRACT “Candidatus Dehalogenimonas etheniformans” strain GP couples growth with the reductive dechlorination of vinyl chloride and several polychlorinated ethenes. The genome sequence comprises a circular 2.07-Mb chromosome with a G+C content of 51.9% and harbors 50 putative reductive dehalogenase genes.

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