scholarly journals Inhibition of Aspergillus niger Phosphate Solubilization by Fluoride Released from Rock Phosphate

2013 ◽  
Vol 79 (16) ◽  
pp. 4906-4913 ◽  
Author(s):  
G. d. O. Mendes ◽  
N. B. Vassilev ◽  
V. H. A. Bonduki ◽  
I. R. da Silva ◽  
J. I. Ribeiro ◽  
...  
1995 ◽  
Vol 44 (3-4) ◽  
pp. 546-549 ◽  
Author(s):  
N. Vassilev ◽  
M. T. Baca ◽  
M. Vassileva ◽  
I. Franco ◽  
R. Azcon

1995 ◽  
Vol 44 (3-4) ◽  
pp. 546-549 ◽  
Author(s):  
N. Vassilev ◽  
M. T. Baca ◽  
M. Vassileva ◽  
I. Franco ◽  
R. Azcon

2007 ◽  
Vol 26 (3) ◽  
pp. 332-336 ◽  
Author(s):  
Nikolay Vassilev ◽  
Maria Vassileva ◽  
Vicente Bravo ◽  
Mercedes Fernández-Serrano ◽  
Iana Nikolaeva

2014 ◽  
Vol 80 (10) ◽  
pp. 3081-3085 ◽  
Author(s):  
Gilberto de Oliveira Mendes ◽  
David Lopez Zafra ◽  
Nikolay Bojkov Vassilev ◽  
Ivo Ribeiro Silva ◽  
José Ivo Ribeiro ◽  
...  

ABSTRACTDuring fungal rock phosphate (RP) solubilization, a significant quantity of fluoride (F−) is released together with phosphorus (P), strongly inhibiting the process. In the present study, the effect of two F−adsorbents [activated alumina (Al2O3) and biochar] on RP solubilization byAspergillus nigerwas examined. Al2O3adsorbed part of the F−released but also adsorbed soluble P, which makes it inappropriate for microbial RP solubilization systems. In contrast, biochar adsorbed only F−while enhancing phosphate solubilization 3-fold, leading to the accumulation of up to 160 mg of P per liter. By comparing the values of F−measured in solution at the end of incubation and those from a predictive model, it was estimated that up to 19 mg of F−per liter can be removed from solution by biochar when added at 3 g liter−1to the culture medium. Thus, biochar acted as an F−sink during RP solubilization and led to an F−concentration in solution that was less inhibitory to the process. In the presence of biochar,A. nigerproduced larger amounts of citric, gluconic, and oxalic acids, whether RP was present or not. Our results show that biochar enhances RP solubilization through two interrelated processes: partial removal of the released F−and increased organic acid production. Given the importance of organic acids for P solubilization and that most of the RPs contain high concentrations of F−, the proposed solubilization system offers an important technological improvement for the microbial production of soluble P fertilizers from RP.


2005 ◽  
Vol 7 (2) ◽  
pp. 77-80
Author(s):  
Yudi Sastro ◽  
Donny Widianto ◽  
Irfan D Prijambada

The study is intended to examine the survival of Aspergillus niger and its phosphate solubilizing ability when pelletedwith rock phosphate. An A. niger YD 17 obtainedfrom the Laboratory of Microbiology. Faculty of Agriculture, Gadjah MadaUniversity was used. The pellet was made by mixing rock phosphate (80%) with organic matter (J 3. 9% waste of tapioca. 6% rice bran, and 1% starch) and spore of A. niger. The experimental design was the Complete Randomized Design 5x4 with 3 replicatiOns. The first factor was sources of rock phosphate (Christmas Island. Jordan, China, Ciamis, and Madura). The secondfactor was the number ofinoculums i.e. control without inoculums, 107 • J(t and J(t cfu.g·'. The colony of A. niger that formed at medium of potatoes dextrose agar (PDA) and the amount of soluble phosphorus in the Pikovskaya liquid medium were parameters. Experimental results indicated that sources of rock phosphate and the number of inoculums itif1uence the A. niger survivability and its phosphorus solubilizing ability. Rock phosphate from Ciamis gave the best support for fungus survival and rock phosphate from Christmas Island was the best substrate for phosphate solubilization. The highest soluble phosphate was achieved by ul inoculums.


2014 ◽  
Vol 65 (2) ◽  
pp. 745-751 ◽  
Author(s):  
Samantha Caixeta de Oliveira ◽  
Gilberto de Oliveira Mendes ◽  
Ubiana Cássia da Silva ◽  
Ivo Ribeiro da Silva ◽  
José Ivo Ribeiro Júnior ◽  
...  

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.


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