scholarly journals The Effect of Rock Phosphate and Level of Inoculums on The Survivability of Aspergillus niger and Its Solubilization Ability When Pelleted With Rock Phosphate

2005 ◽  
Vol 7 (2) ◽  
pp. 77-80
Author(s):  
Yudi Sastro ◽  
Donny Widianto ◽  
Irfan D Prijambada
Keyword(s):  
Organic Matter ◽  
Aspergillus Niger ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Soluble Phosphate ◽  
Christmas Island ◽  
Randomized Design ◽  
Soluble Phosphorus ◽  
Phosphate Solubilizing ◽  
Solubilization Ability

The study is intended to examine the survival of Aspergillus niger and its phosphate solubilizing ability when pelletedwith rock phosphate. An A. niger YD 17 obtainedfrom the Laboratory of Microbiology. Faculty of Agriculture, Gadjah MadaUniversity was used. The pellet was made by mixing rock phosphate (80%) with organic matter (J 3. 9% waste of tapioca. 6% rice bran, and 1% starch) and spore of A. niger. The experimental design was the Complete Randomized Design 5x4 with 3 replicatiOns. The first factor was sources of rock phosphate (Christmas Island. Jordan, China, Ciamis, and Madura). The secondfactor was the number ofinoculums i.e. control without inoculums, 107 • J(t and J(t cfu.g·'. The colony of A. niger that formed at medium of potatoes dextrose agar (PDA) and the amount of soluble phosphorus in the Pikovskaya liquid medium were parameters. Experimental results indicated that sources of rock phosphate and the number of inoculums itif1uence the A. niger survivability and its phosphorus solubilizing ability. Rock phosphate from Ciamis gave the best support for fungus survival and rock phosphate from Christmas Island was the best substrate for phosphate solubilization. The highest soluble phosphate was achieved by ul inoculums.

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium

1995 ◽  
Vol 44 (3-4) ◽  
pp. 546-549 ◽  
Author(s):  
N. Vassilev ◽  
M. T. Baca ◽  
M. Vassileva ◽  
I. Franco ◽  
R. Azcon
Keyword(s):  
Aspergillus Niger ◽  
Sugar Beet ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Rock Phosphate Solubilization

Optimization for rock phosphate solubilization by phosphate-solubilizing fungi isolated from phosphate mines

2008 ◽  
Vol 33 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Chun-Qiao Xiao ◽  
Ru-An Chi ◽  
Xiao-Hui Huang ◽  
Wen-Xue Zhang ◽  
Guan-Zhou Qiu ◽  
...  
Keyword(s):  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Rock Phosphate Solubilization ◽  
Phosphate Mines ◽  
Phosphate Solubilizing

scholarly journals Inorganic Phosphate Solubilization by Rhizosphere Bacterium Paenibacillus sonchi: Gene Expression and Physiological Functions

2020 ◽  
Vol 11 ◽  
Author(s):  
Luciana F. Brito ◽  
Marina Gil López ◽  
Lucas Straube ◽  
Luciane M. P. Passaglia ◽  
Volker F. Wendisch
Keyword(s):  
Gene Expression ◽  
Organic Acids ◽  
Phosphate Solubilization ◽  
Tricarboxylic Acid Cycle ◽  
Initial Step ◽  
High Rate ◽  
Soluble Phosphate ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing ◽  
Phosphate Source

Due to the importance of phosphorus (P) in agriculture, crop inoculation with phosphate-solubilizing bacteria is a relevant subject of study. Paenibacillus sonchi genomovar Riograndensis SBR5 is a promising candidate for crop inoculation, as it can fix nitrogen and excrete ammonium at a remarkably high rate. However, its trait of phosphate solubilization (PS) has not yet been studied in detail. Here, differential gene expression and functional analyses were performed to characterize PS in this bacterium. SBR5 was cultivated with two distinct P sources: NaH2PO4 as soluble phosphate source (SPi) and hydroxyapatite as insoluble phosphate source (IPi). Total RNA of SBR5 cultivated in those two conditions was isolated and sequenced, and bacterial growth and product formation were monitored. In the IPi medium, the expression of 68 genes was upregulated, whereas 100 genes were downregulated. Among those, genes involved in carbon metabolism, including those coding for subunits of 2-oxoglutarate dehydrogenase, were identified. Quantitation of organic acids showed that the production of tricarboxylic acid cycle-derived organic acids was reduced in IPi condition, whereas acetate and gluconate were overproduced. Increased concentrations of proline, trehalose, and glycine betaine revealed active osmoprotection during growth in IPi. The cultivation with hydroxyapatite also caused the reduction in the motility of SBR5 cells as a response to Pi depletion at the beginning of its growth. SBR5 was able to solubilize hydroxyapatite, which suggests that this organism is a promising phosphate-solubilizing bacterium. Our findings are the initial step in the elucidation of the PS process in P. sonchi SBR5 and will be a valuable groundwork for further studies of this organism as a plant growth-promoting rhizobacterium.

Dynamic of Chemical and Biological Properties of Inceptisols Cilembu due to Phosphate Rock Nanoparticle Amendment and Phosphate-Solubilizing Fungi Inoculation

2021 ◽  
Vol 1044 ◽  
pp. 121-131
Author(s):  
Pujawati Suryatmana ◽  
Muhammad Amir Solihin ◽  
Rina Devnita ◽  
Fajri Syahid Nurhakim ◽  
Apong Sandrawati ◽  
...  
Keyword(s):  
Phosphate Rock ◽  
Rock Phosphate ◽  
Biological Properties ◽  
Exchange Capacity ◽  
Limiting Factors ◽  
Available P ◽  
Independent Effect ◽  
Natural Material ◽  
Randomized Design ◽  
Phosphate Solubilizing

Cilembu's Inceptisols have great potential for agriculture. However, land management has several limiting factors: the available phosphate content, cation exchange capacity (CEC), and low population of functional soil organisms. Rock phosphate is a natural material that can increase the availability of P nutrient. Application of rock phosphate as an ameliorant in nanoparticle size and inoculation of phosphate-solubilizing fungi (PSF) is a strategy that can be implemented to address the Inceptisols constraints. However, the dynamic of chemical and biological characteristics of Cilembu Inceptisols due to amendment of phosphate rock nanoparticle (PRNp) and phosphate solubilizing fungi (PSF) inoculation is not yet understood clearly. To know the dynamics of the chemical and biological properties of the soil due to the treatment of these materials is an essential aspect for strategy and planning in its proper application to improve the properties of Inceptisols. The completely randomized design (CRD) was used in this study, with a factorial pattern, consisting of the first factor being the amendment of rock phosphate nanoparticle and the second factor being the PSF inoculation. The observation was carried out in a month interval, with a three-month incubation. The result showed there was no interaction between phosphate rock nanoparticle amendment and PSF inoculation on pH-H2O, available P, CEC, and abundance of PSF population. The independent effect showed that a characteristic pattern of pH and available P values during two months of observation was increasing by PRNp amendment. Meanwhile, inoculation of PSF did not increase soil pH and P-available. The value of CEC and the population of PSF did not increase significantly during the three-month incubation. The dose of phosphate rock nanoparticle 2% (wt/wt) increased the highest available P.

scholarly journals Inhibition of Aspergillus niger Phosphate Solubilization by Fluoride Released from Rock Phosphate

2013 ◽  
Vol 79 (16) ◽  
pp. 4906-4913 ◽  
Author(s):  
G. d. O. Mendes ◽  
N. B. Vassilev ◽  
V. H. A. Bonduki ◽  
I. R. da Silva ◽  
J. I. Ribeiro ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Rock Phosphate ◽  
Phosphate Solubilization

Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium

1995 ◽  
Vol 44 (3-4) ◽  
pp. 546-549 ◽  
Author(s):  
N. Vassilev ◽  
M. T. Baca ◽  
M. Vassileva ◽  
I. Franco ◽  
R. Azcon
Keyword(s):  
Aspergillus Niger ◽  
Sugar Beet ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Rock Phosphate Solubilization

scholarly journals Phosphate-solubilizing fungi isolated from a semiarid area cultivated with melon (Cucumis melo L. cv. gold mine)

2011 ◽  
Vol 25 (4) ◽  
pp. 929-931 ◽  
Author(s):  
Flavia Paiva Coutinho ◽  
Maria Auxiliadora de Queiroz Cavalcanti ◽  
Adriana Mayumi Yano-Melo
Keyword(s):  
Cucumis Melo ◽  
Phosphate Solubilization ◽  
Screening Program ◽  
Gold Mine ◽  
Semiarid Area ◽  
Fungal Isolates ◽  
Phosphate Solubilizing ◽  
Cucumis Melo L ◽  
Solubilization Ability ◽  
Mycelia Sterilia

Considering that little is known about the occurrence of phosphate-solubilizing fungi from areas cultivated with melon, the phosphate solubilization ability of filamentous fungi isolated in these areas was evaluated. Three hundred and eighteen filamentous fungal isolates belonging to 23 genera were evaluated, besides Aphyllophorales and Mycelia sterilia. From those, 52 were able to solubilize P: Aphyllophorales (2), Aspergillus (34), Penicillium (10) and Rhizopus (6). These results will contribute to subsidizing further research regarding the capacity of these fungi to solubilize other sources of phosphate applied to the melon crop, as well as indicate the need for a screening program to select those with higher capacity and potential for solubilization.

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