scholarly journals Nature and Role of Bacterial Contaminants in Mass Cultures of Thermophilic Chlorella pyrenoidosa

1965 ◽  
Vol 13 (3) ◽  
pp. 473-477 ◽  
Author(s):  
Richard J. Blasco
1966 ◽  
Vol 165 (1001) ◽  
pp. 473-485 ◽  

The photometabolism of glucose by Chlorella pyrenoidosa was studied by following the fate of exogenously supplied glucose-1- 14 C, glucose-2- 14 C and glucose-6- 14 C. The sucrose and insoluble polyglucan formed were extracted and hydrolysed. The constituent glucose units were degraded to determine the distribution of radioactivity between the six carbon atoms of the glucose chain. Formation of glycollic acid and of glycine was stimulated by a gas stream of 100% oxygen and by adding isonicotinyl hydrazide ( INH ). Although increases of glycollic acid and glycine were observed as a result of these treatments and at the expense of both sucrose and polyglucan the distribution of 14 C between the carbon atoms of the glucose units was scarcely affected. The results are discussed with particular reference to the metabolism of glycollic acid in Chlorella .


Author(s):  
Dussart Claude ◽  
Boulliat Caroline ◽  
Camal Isabelle ◽  
Bourgeois Denis ◽  
Carrouel Florence

This study aims to evaluate the potential role of pillboxes used for the preparation and delivery of individual daily medical treatments in the drug circuit of the Military Instruction Hospital (France) as reservoirs of bacterial contaminants. Samples were obtained from 32 pillboxes after decontamination (T1), after preparation in the pharmacy (T2), after use in two different medical units (T3), and again after usual mechanical washing (T4). Qualitative (identification and antibiotic susceptibility) and quantitative (contamination rate and number of colony forming units—CFUs) bacteriological tests were performed. Susceptible and resistant strains of environmental saprophytes were identified. The pillbox contamination rate was relatively low at T1 (13%). It was significantly increased at T2 (63%, p = 0.001 vs. T1), again at T3 (88%, p < 0.05 vs. T2, p < 0.001 vs. T1), and finally decreased dramatically at T4 (31%, p < 0.001 vs. T3, p > 0.05 vs. T1). The number of CFUs was significantly increased at T2 compared with that of T1 (36.7 ± 13.4 and 5.36 ± 3.64, respectively, p < 0.001) and again at T3 (84.4 ± 19.4, p < 0.001 vs. T1 and T2) and was significantly reduced at T4 (7.0 ± 2.0 vs. T3, p < 0.001) to a level that was not significantly different from that at T1. So, the use of pillboxes to deliver individual medications to patients in the hospital is a potential risk factor for bacterial cross-contamination.


2017 ◽  
Vol 6 (1) ◽  
pp. 774-780 ◽  
Author(s):  
Anwesha Khanra ◽  
Sujata Sangam ◽  
Adeeba Shakeel ◽  
Deepa Suhag ◽  
Subhradeep Mistry ◽  
...  

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Arghya Bhattacharya ◽  
Megha Mathur ◽  
Pushpendar Kumar ◽  
Anushree Malik

The investigations of two light reactions in photosynthesis driven by different pigment systems started with Emerson’s discovery that in the green alga Chlorella pyrenoidosa the rate of photosynthesis in light beams of 700 and 650 applied simultaneously, was higher than the sum of the rates in each beam separately (Emerson, Chalmers & Cederstrand 1957). This effect has been called ‘Emerson effect’ or ‘enhancement effect’. Since the quantum efficiency of light of 700 mp, compared with that of 650 mpwas rather low, the increase in rate in the two beams was attributed to an enhancement of the photosynthetic efficiency of light of 700 nip, by light of 650 mp. In this paper we will present evidence that the rate of photosynthesis at both wavelengths is enhanced. Action spectra for the Emerson effect were determined by measuring the extra increase in photosynthesis obtained by adding light of various wavelengths to constant illumination of 700 mp. The action spectrum for the Emerson effect in Chlorella showed maxima at 650 and 480 mp, which indicated that light absorbed by chlorophyll b was particularly effective in the enhancement. In the red alga Porphyridium cruentum photosynthesis brought about by light absorbed in the region around 680 mp was ‘enhanced’ by light absorbed by the phycobilins phycocyanin and phycoerythrin (Brody & Emerson 1959). It was concluded that efficient photosynthesis was only possible, if at least one of the accessory pigments was excited (Emerson et al. 1957; Brody & Emerson 1959).


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