scholarly journals Seasonal variation in survival of Escherichia coli exposed in situ in membrane diffusion chambers containing filtered and nonfiltered estuarine water.

1983 ◽  
Vol 45 (6) ◽  
pp. 1877-1883 ◽  
Author(s):  
I C Anderson ◽  
M W Rhodes ◽  
H I Kator
Keyword(s):  
Escherichia Coli ◽  
Seasonal Variation ◽  
Estuarine Water ◽  
Membrane Diffusion ◽  
Diffusion Chambers

A survival study of Escherichia coli in a south African river using membrane diffusion chambers

1995 ◽  
Vol 31 (5-6) ◽  
pp. 185-188 ◽  
Author(s):  
C. M. E. de Wet ◽  
S. N. Venter ◽  
N. Rodda ◽  
R. Kfir ◽  
M. C. Steynberg ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South African ◽  
The Other ◽  
Membrane Diffusion ◽  
Diffusion Chambers ◽  
E Coli ◽  
Significant Difference ◽  
Survival Pattern ◽  
The One ◽  
Different Parts

Studies to describe the survival of Escherichia coli were performed at two sites in a river. The one site was dominated by domestic discharge and the other by industrial inputs. E coli suspensions within membrane diffusion chambers were immersed in the river at the selected sites. An identical chamber was submerged in river water in the laboratory as a comparison. Two test runs were performed, one during winter (July) and one during summer (December). Samples to determine the survival of E coli was taken on a scheduled basis. Results obtained showed no significant difference between the survival pattern of E coli as determined during the summer and winter periods or in the different parts of the river. The same survival pattern was observed for the studies performed in the laboratory.

scholarly journals In situ delivery of biobutyrate by probiotic Escherichia coli for cancer therapy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chung-Jen Chiang ◽  
Yan-Hong Hong
Keyword(s):  
Escherichia Coli ◽  
Cancer Therapy ◽  
Rational Design ◽  
Hypoxic Condition ◽  
Human Colorectal Cancer ◽  
Apoptosis Pathway ◽  
Mitochondrial Apoptosis Pathway ◽  
Cycle Arrest ◽  
Human Colorectal Cancer Cell

AbstractButyrate has a bioactive function to reduce carcinogenesis. To achieve targeted cancer therapy, this study developed bacterial cancer therapy (BCT) with butyrate as a payload. By metabolic engineering, Escherichia coli Nissle 1917 (EcN) was reprogrammed to synthesize butyrate (referred to as biobutyrate) and designated EcN-BUT. The adopted strategy includes construction of a synthetic pathway for biobutyrate and the rational design of central metabolism to increase the production of biobutyrate at the expense of acetate. With glucose, EcN-BUT produced primarily biobutyrate under the hypoxic condition. Furthermore, human colorectal cancer cell was administrated with the produced biobutyrate. It caused the cell cycle arrest at the G1 phase and induced the mitochondrial apoptosis pathway independent of p53. In the tumor-bearing mice, the injected EcN-BUT exhibited tumor-specific colonization and significantly reduced the tumor volume by 70%. Overall, this study opens a new avenue for BCT based on biobutyrate.

scholarly journals Characteristics of Diurnal Rainfall over Peatland Area of South Sumatra, Indonesia

2020 ◽  
Vol 5 (4) ◽  
pp. 136
Author(s):  
Puad Maulana Mandailing ◽  
Wijaya Mardiansyah ◽  
Muhammad Irfan ◽  
Arsali Arsali ◽  
Iskhaq Iskandar
Keyword(s):  
Seasonal Variation ◽  
Early Morning ◽  
Temporal Variations ◽  
In Situ Observation ◽  
Peak Time ◽  
The South ◽  
Night Time ◽  
Late Night ◽  
Diurnal Rainfall

The peak time of rainfall occurrence over an area has certain characteristics in which the difference in time and intensity of rainfall varies depending on its location and distance from the sea. This variation can be determined based on the phase and amplitude obtained using harmonic analysis. In this study, combined data from in-situ observation, satellite remote sensing and reanalysis were used to analyze spatial and temporal variations of peak rainfall events over peatland area of the South Sumatra Province. The results show that most of the South Sumatra Province has a diurnal peak of rainfall during afternoon ranging from 16.00 to 19.00 Western Indonesian Time. In addition, the results also indicate that the analysis on the in situ data revealed seasonal variation both in amplitude and time of maximum diurnal rainfall, while the reanalysis data only indicated a weak seasonal variation on the amplitude of the diurnal rainfall. Furthermore, spatial analysis shows that the time of maximum diurnal rainfall has spatial variation. Over the ocean, the time of maximum diurnal rainfall occurs during night time/early morning. Over the lowland or coastal area, the time of maximum diurnal rainfall occurs during afternoon, while over the high altitude (mountain) it occurs during late night.

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