Biological Control of Agrobacterium tumefaciens, Colonization, and pAgK84 Transfer with Agrobacterium radiobacter K84 and the Tra- Mutant Strain K1026

The hypothesis has been advanced that the inhibition of phosphorylase by uridine diphosphate glucose (UDPG), together with the fact that the latter compound is the substrate for glycogen synthetase, is the basis of a mechanism for the biological control of glycogen metabolism in Agrobacterium tumefaciens. Experiments were designed to test this hypothesis on the assumption that such a control mechanism would manifest itself by the concentrations of UDPG and glycogen bearing some relationship to each other during various stages of growth and nutrition. Glycogen levels in the cells increased markedly during the lag phase of growth, decreased during the exponential growth phase, and increased again as growth ceased due to the depletion of nitrogen from the medium (nitrogen starvation). The UDPG concentration paralleled these changes, and a high positive correlation between the concentrations of UDPG and glycogen was demonstrated. The addition of ammonium chloride to nitrogen-starved cells caused a prompt resumption of growth, a sharp decrease in the UDPG concentration, and a somewhat smaller decrease in glycogen concentration. Cells placed in buffered salt solution and aerated had a low concentration of UDPG and exhibited a steady decline of the glycogen reserves. Although factors other than those considered here may also be of importance in the control of glycogen metabolism in this organism, the data in general support the hypothesis advanced above.

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Minor structures in β-d-(1→2)-linked d-glucopyranans from Agrobacterium tumefaciens and Agrobacterium radiobacter

Carbohydrate Research ◽

10.1016/s0008-6215(00)85712-2 ◽

1980 ◽

Vol 82 (2) ◽

pp. 366-371 ◽

Cited By ~ 11

Author(s):

Eliana Barreto-Bergter ◽

Celina Raquel Camargo ◽

Lawrence R. Hogge ◽

Philip A.J. Gorin

Keyword(s):

Agrobacterium Tumefaciens ◽

Agrobacterium Radiobacter

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Screening and characterization of an Agrobacterium tumefaciens mutant strain producing high level of coenzyme Q10

Process Biochemistry ◽

10.1016/j.procbio.2014.10.024 ◽

2015 ◽

Vol 50 (1) ◽

pp. 33-39 ◽

Cited By ~ 4

Author(s):

Tae-Su Kim ◽

Ji-Hyun Yoo ◽

Sang-Yong Kim ◽

Cheol-Ho Pan ◽

Vipin C. Kalia ◽

...

Keyword(s):

Agrobacterium Tumefaciens ◽

Mutant Strain ◽

Coenzyme Q10 ◽

High Level

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Auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of Agrobacterium tumefaciens

Planta ◽

10.1007/bf00198948 ◽

1992 ◽

Vol 188 (1) ◽

Author(s):

BruceR. Campell ◽

Ling-Yuan Su ◽

WilliamL. Pengelly

Keyword(s):

Agrobacterium Tumefaciens ◽

Mutant Strain

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Biological Control of Crown Gall on Peach and Cherry Rootstock Colt by Native Agrobacterium radiobacter Isolates~!2009-05-11~!2009-08-25~!2010-02-11~!

The Open Horticulture Journal ◽

10.2174/1874840601003010001 ◽

2010 ◽

Vol 3 (1) ◽

pp. 1-10 ◽

Cited By ~ 6

Author(s):

A. K. Gupta ◽

Kishore Khosla ◽

S. S. Bhardwaj ◽

Aman Thakur ◽

Sapna Devi ◽

...

Keyword(s):

Biological Control ◽

Crown Gall ◽

Agrobacterium Radiobacter

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Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/607329 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Yahong Yuan ◽

Yuting Tian ◽

Tianli Yue

Keyword(s):

Agrobacterium Tumefaciens ◽

Hydrostatic Pressure ◽

Batch Culture ◽

Mutant Strain ◽

Coenzyme Q10 ◽

High Hydrostatic Pressure ◽

Original Strain ◽

Fed Batch ◽

Fed Batch Culture ◽

Coq10 Production

Coenzyme Q10 (CoQ10, ubiquinone), a potent antioxidative dietary supplement, was produced by submerged fermentation usingAgrobacterium tumefaciensinstead of chemical synthesis or solvent extraction.Agrobacterium tumefaciens1.2554 was subjected to mutagenesis using a series of treatments including high hydrostatic pressure (HHP) treatment, UV irradiation, and diethyl sulfate (DES) treatment to obtain mutant strains showing higher CoQ10 production than wild-type strains. A mutant strain PK38 with four genetic markers was isolated: the specific CoQ10 content of the mutant strain increased by 52.83% compared with the original strain. Effects of carbon and nitrogen sources on CoQ10 production with PK38 were studied. Sucrose at concentration of 30 g/l was tested as the best carbon source, and yeast extract at concentration of 30 g/l supplemented with 10 g/l of ammonium sulfate was identified to be the most favorable for CoQ10 production using PK38. Fed-batch culture strategy was then used for increasing production of CoQ10 in 5-l fermentor. Using the exponential feeding fed-batch culture of sucrose, cell growth and CoQ10 formation were significantly improved. With this strategy, the final cell biomass, CoQ10 production, and specific CoQ10 production increased by 126.11, 173.12, and 22.76%, respectively, compared to those of batch culture.

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Antagonistic potential of native agrocin-producing non-pathogenic Agrobacterium tumefaciens strain UHFBA-218 to control crown gall in peach

Phytoprotection ◽

10.7202/1040509ar ◽

2017 ◽

Vol 97 (1) ◽

pp. 1-11

Author(s):

Aditi Sharma ◽

A. K. Gupta ◽

K. Khosla ◽

Rishi Mahajan ◽

Bharti ◽

...

Keyword(s):

Biological Control ◽

Agrobacterium Tumefaciens ◽

Crown Gall ◽

Room Temperature ◽

Biological Control Agent ◽

Control Agent ◽

Stone Fruits ◽

Copper Oxychloride ◽

Antagonistic Potential ◽

Different Doses

A non-pathogenic agrocin-producing native isolate ofAgrobacterium tumefaciensstrain UHFBA-218 was tested as a biological control agent against the peach crown gall. This strain was compatible with all the recommended pesticides used in stone fruits in the integrated pest management (IPM) module, except for copper oxychloride, which was detrimental to its growth. Upon artificial co-inoculation of 4-wk-old plants of tomato var. Solan Gola withA. tumefaciensstrain UHFBA-218 and tumorigenicA. tumefaciensstrain Peach 2E-10, out of the 27 isolates recovered, six were transconjugants showing selective acquisition of tumorigenic factors as made evident by amplification withiptandvirD2primers, whereas the rest of the isolates did not acquire any of these tumorigenic factors. A white stone powder-based formulation of this isolate (103.3 × 108cfu g-1) retained appreciable viability for up to 6 months at room temperature. When peach roots and seeds were soaked in cell suspensions of different doses of a white stone powder-based bioformulation of UHFBA-218 before planting in the field, the number of plants with tumours was reduced, with the lowest incidence of crown gall being observed in the 0.1% UHFBA-218 root dip treatment, i.e. 1.48% and 0.80% during the years 2013 and 2014, respectively. No incidence of crown gall was observed in the three seed dip treatments, i.e. 30-min dip in UHFBA-218 followed by 1 h of shade drying, stratified seeds dipped for 30 min in 0.1% suspensions of strains UHFBA-218 or K84 followed by 1 h of shade drying before sowing, as compared with 14.76% incidence in untreated plants.

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Proposal that Agrobacterium radiobacter has priority over Agrobacterium tumefaciens. Request for an Opinion

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64030-0 ◽

2006 ◽

Vol 56 (2) ◽

pp. 491-493 ◽

Cited By ~ 21

Author(s):

J. M. Young ◽

S. R. Pennycook ◽

D. R. W. Watson

Keyword(s):

Agrobacterium Tumefaciens ◽

Type Species ◽

Agrobacterium Radiobacter ◽

Subjective Synonym

It is proposed that Agrobacterium radiobacter has priority as the earlier heterotypic (subjective) synonym when it is united with Agrobacterium tumefaciens. The nomenclatural status of A. tumefaciens as a later heterotypic synonym of the united species is not lost and it remains the type species of the genus. Request for an Opinion.

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